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Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial 肌醇与二甲双胍对多囊卵巢综合征不孕妇女临床特征、内分泌和代谢特征的影响:随机对照试验
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390300
N.S.V. Le, Minh Tam Le, T. N. Cao
To compare the effectiveness of inositol and metformin on the clinical characteristics, and endocrine and metabolic profiles of infertile polycystic ovarian syndrome (PCOS) women from Vietnam. From June 2018 to August 2022, a randomized trial was undertaken at the Hue Center for Endocrinology and Reproduction on infertile women aged 18 to 40 years with polycystic ovarian syndrome. The clinical, endocrine, and metabolic features of these individuals were assessed before and after 3 months of treatment with 2 g of inositol or 1700 mg of metformin per day. Natural pregnancy rates, adverse effects, and tolerance of inositol were recorded. The study included 171 infertile PCOS women who were eligible to participate and took part in the baseline assessment, of whom 132 women participated in data analysis after 3 months. After metformin treatment, 42.1% of women with oligomenorrhea experienced regular menstruation. Metformin significantly lowered body mass index (BMI), waist circumference and testosterone levels, but had no effect on other clinical characteristics, endocrine profiles, or metabolic profiles. 29.2% Of women reported experiencing side effects. 21% Of them attained pregnancy, which resulted in 17.1% of live births. In the inositol group, the rate of regular cycle increased by 18.2% and the total testosterone concentration significantly decreased. In overweight/obese women with PCOS, inositol significantly decreased weight, BMI, waist and hip circumferences (P<0.05). 100% Of women tolerated inositol and continued treatment. 18.9% Of them became pregnant, leading to 17% of live births. Metformin and inositol can improve weight and waist circumference in overweight/obese infertile women with PCOS. Metformin is associated with a higher rate of regular menstruation, whereas inositol is associated with a lower rate of adverse effects. The spontaneous conception, clinical pregnancy, and live birth rates between two groups are comparable.
目的:比较肌醇和二甲双胍对越南多囊卵巢综合征(PCOS)不孕妇女的临床特征、内分泌和代谢特征的有效性。 2018年6月至2022年8月,顺化内分泌与生殖中心对18至40岁患有多囊卵巢综合征的不孕妇女进行了随机试验。在每天服用 2 克肌醇或 1700 毫克二甲双胍治疗 3 个月前后,对这些人的临床、内分泌和代谢特征进行了评估。同时还记录了自然怀孕率、不良反应以及对肌醇的耐受性。 该研究包括171名符合条件并参加了基线评估的多囊卵巢综合征不孕妇女,其中132名妇女参加了3个月后的数据分析。接受二甲双胍治疗后,42.1%的少经妇女月经正常。二甲双胍能明显降低体重指数(BMI)、腰围和睾酮水平,但对其他临床特征、内分泌特征或代谢特征没有影响。29.2%的妇女表示出现了副作用。其中 21% 的妇女怀孕,活产率为 17.1%。在肌醇组中,正常周期的比率增加了 18.2%,总睾酮浓度显著下降。在患有多囊卵巢综合症的超重/肥胖妇女中,肌醇能明显降低体重、体重指数、腰围和臀围(P<0.05)。100%的妇女能够耐受肌醇并继续接受治疗。其中 18.9% 的妇女怀孕,活产率为 17%。 二甲双胍和肌醇可以改善患有多囊卵巢综合症的超重/肥胖不孕妇女的体重和腰围。二甲双胍与较高的月经规律率有关,而肌醇与较低的不良反应率有关。两组患者的自然受孕率、临床妊娠率和活产率相当。
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引用次数: 0
Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study 新鲜卵母细胞与玻璃化卵母细胞的胚胎、遗传和临床结果:回顾性队列研究
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390301
Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh
To compare embryonic development, ploidy status and clinical outcomes between fresh and frozen-thawed oocytes. This retrospective cohort study evaluated 83 fertilization cycles including both fresh and frozen oocytes from 79 patients at the HP Fertility Center of Hai Phong International Hospital of Obstetrics and Pediatrics in Vietnam. The patient underwent several ovarian stimulation cycles to accumulate a certain number of oocytes that would be vitrified. In the last oocyte retrieval, all patient's oocytes including both frozen and fresh would be fertilized. The outcomes included the rates of oocyte survival, cleavage embryo, blastocyst, ploidy status, pregnancy, biochemical pregnancy and clinical pregnancy. The oocyte survival rate after thawing was 96.5%. No statistically significant difference was found when comparing fresh and frozen oocytes regarding fertilization rate (78.1% vs. 75.5%, P=0.461), usable cleavage embryo rate (86.9% vs. 87.2%, P=0.916) but usable blastocyst rate was found higher statistically in the frozen oocyte group (44.4% vs. 54.0%, P=0.049). The percentages of euploid, aneuploid and mosaic embryos between the fresh group and the vitrified group had no significant differences (33.8% vs. 31.6%, P=0.682; 51.0% vs. 54.2%, P=0.569; 15.2% vs. 12.4%, P=0.787; respectively). The rates of pregnancy, biochemical pregnancy and clinical pregnancy had no statistical difference (68.8% vs. 64.8%, P=0.764; 12.5% vs. 3.6%, P=0.258; 37.5% vs. 46.4%, P=0.565). 17 Mature oocytes are the minimum to have at least one euploid embryo. Oocyte vitrification does not affect embryonic, genetic and clinical results. The number of mature oocytes should be considered for fertilization in some cases.
比较新鲜卵母细胞和冷冻解冻卵母细胞的胚胎发育、倍性状态和临床结果。 这项回顾性队列研究评估了越南海防国际产科和儿科医院 HP 生育中心 79 名患者的 83 个受精周期,包括新鲜和冷冻卵母细胞。患者接受了多个卵巢刺激周期,以积累一定数量的卵母细胞进行玻璃化处理。在最后一次取卵过程中,患者的所有卵母细胞(包括冷冻和新鲜卵母细胞)都将受精。结果包括卵母细胞存活率、卵裂胚胎率、囊胚率、倍性状态、妊娠率、生化妊娠率和临床妊娠率。 解冻后的卵母细胞存活率为 96.5%。新鲜卵母细胞和冷冻卵母细胞在受精率(78.1% 对 75.5%,P=0.461)和可用卵裂胚胎率(86.9% 对 87.2%,P=0.916)方面的差异无统计学意义,但冷冻卵母细胞组的可用囊胚率较高(44.4% 对 54.0%,P=0.049)。新鲜胚胎组和玻璃化胚胎组的非整倍体胚胎、非整倍体胚胎和马赛克胚胎的百分比没有显著差异(分别为 33.8% vs. 31.6%,P=0.682;51.0% vs. 54.2%,P=0.569;15.2% vs. 12.4%,P=0.787)。妊娠率、生化妊娠率和临床妊娠率无统计学差异(68.8% vs. 64.8%,P=0.764;12.5% vs. 3.6%,P=0.258;37.5% vs. 46.4%,P=0.565)。17 成熟卵母细胞至少要有一个单倍体胚胎。 卵母细胞玻璃化不会影响胚胎、遗传和临床结果。在某些情况下,受精时应考虑成熟卵母细胞的数量。
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引用次数: 0
Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro 低温保存的绵羊精原干细胞在体外保持干性和集落形成能力
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390302
R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra
To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells (SSCs) in vitro. Sheep testicular cells were isolated and putative SSCs were enriched by the laminin-based differential plating method. Putative SSCs were co-cultured with the Sertoli cell feeder prepared by the Datura Stramonium Agglutinin (DSA-lectin)-based method. The cultured putative SSCs were cryopreserved in Dulbecco's Modified Eagle Medium-10% fetal bovine serum mixture (DMEM-10% FBS) media containing 10% dimethyl sulfoxide (DMSO) alone or 10% DMSO plus 200 mM trehalose. Cryopreserved putative SSCs were evaluated for their proliferation potential using in vitro culture and stemness by immunocytochemistry. Finally, the transfection ability of cryopreserved putative SSCs was analyzed. We isolated 91% viable testicular cells from sheep testes. The majority of the laminin enriched cells expressed the SSC related marker, ITGA6. Co-culture of sheep putative SSCs with Sertoli cell feeder resulted in the generation of stable colonies, and the expression of SSC marker was maintained after several passages. A significantly higher number of viable putative SSCs was recovered from SSCs cryopreserved in media containing 10% DMSO and 200 mM trehalose compared to 10% DMSO alone (P<0.01). Cryopreserved putative SSCs formed colonies and showed SSC marker expression similar to the non-cryopreserved putative SSCs. The appearance of green fluorescent colonies over the Sertoli cell feeder indicated that cryopreserved sheep SSCs were successfully transfected. Cryopreserved putative SSCs can retain their stemness, colony forming ability, and transfection efficiency in vitro. Our research may help in the effective preservation of germplasm and the generation of transgenic ovine species.
评估低温保存对体外绵羊精原干细胞(SSCs)的干性和增殖潜力的影响。 分离绵羊睾丸细胞,并通过基于层粘连素的差分平板法富集推定的精原干细胞。将推定的SSCs与基于曼陀罗凝集素(DSA-lectin)方法制备的Sertoli细胞供养体共同培养。培养出的推定 SSCs 被冷冻保存在含 10%二甲基亚砜(DMSO)或 10%二甲基亚砜加 200 mM 曲哈糖的杜氏改良鹰培养基-10%胎牛血清混合液(DMEM-10% FBS)培养基中。利用体外培养和免疫细胞化学方法对冷冻保存的推定 SSCs 的增殖潜力和干性进行评估。最后,分析了冷冻保存的推定 SSCs 的转染能力。 我们从绵羊睾丸中分离出了91%存活的睾丸细胞。大部分富含层粘连蛋白的细胞表达了与SSC相关的标志物ITGA6。绵羊推定SSC细胞与Sertoli细胞饲养剂共培养可产生稳定的集落,SSC标记的表达在多次传代后仍能保持。在含10%二甲基亚砜和200毫摩尔曲哈洛糖的培养基中冷冻保存的SSC与仅含10%二甲基亚砜的培养基相比,能存活的推定SSC数量明显更高(P<0.01)。冷冻保存的假定 SSCs 形成了菌落,并显示出与非冷冻保存的假定 SSCs 相似的 SSC 标记表达。Sertoli细胞饲养器上出现绿色荧光菌落表明冷冻保存的绵羊SSCs转染成功。 低温保存的推定造血干细胞可保持其干性、集落形成能力和体外转染效率。我们的研究可能有助于种质的有效保存和转基因绵羊的产生。
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引用次数: 0
Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands 季节应激对安达曼和尼科巴群岛濒危泰瑞莎山羊精液质量、精液生化和氧化应激属性的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390304
P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar
To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons. A total of 10 Teressa bucks (3-4 years) were selected from breeding farm, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India. Semen samples (n=25 per season) were collected through artificial vagina method and preserved at refrigerated temperature (5°C) for 48 h using Tris citrate glucose based extender. We detected semen quality parameters [volume, mass activity, pH, sperm concentration, total motility, viability, total sperm abnormality, and plasma membrane, acrosomal and nuclear integrities], biochemical profiles [aspartate amino transferase (AST), alanine amino transferase (ALT) and total cholesterol], and oxidative stress markers [total antioxidant capacity (TAC) and malondialdehyde (MDA)] during monsoon and dry seasons. Semen quality parameters significantly differed between seasons (P<0.05) and among storage periods (P<0.05). Volume, pH, mass activity, motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly higher (P<0.05). Sperm concentration, sperm abnormalities, MDA, AST, ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season (P<0.05). Motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly decreased (P<0.05) while sperm abnormality, AST, ALT, total cholesterol and MDA were significantly increased as liquid semen storage period advanced (P<0.05). Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h. Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination.
测量季风和干旱季节对新鲜精液和液体储存精液的精液质量、精液生化和氧化应激属性的季节性影响。 从印度安达曼和尼科巴群岛布莱尔港的ICAR-中央岛屿农业研究所繁殖场选取了10头Teressa公鹿(3-4岁)。通过人工阴道法采集精液样本(每季 25 份),并使用柠檬酸特里斯葡萄糖基延长剂在冷藏温度(5°C)下保存 48 小时。我们检测了季风季节和旱季的精液质量参数[体积、质量活性、pH值、精子浓度、总活力、存活率、精子畸形总数以及质膜、顶体和核完整性]、生化指标[天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)和总胆固醇]以及氧化应激指标[总抗氧化能力(TAC)和丙二醛(MDA)]。 精液质量参数在不同季节(P<0.05)和不同储存期(P<0.05)之间存在明显差异。体积、pH值、质量活性、运动性、存活率、顶体、质膜和核整合度以及TAC都明显较高(P<0.05)。季风季节新鲜精液中的精子浓度、精子畸形率、MDA、AST、ALT 和总胆固醇明显低于旱季(P<0.05)。随着精液贮存期的延长,精子活力、顶体、质膜和核完整性以及TAC明显降低(P<0.05),而精子畸形率、谷草转氨酶、谷丙转氨酶、总胆固醇和MDA则明显升高(P<0.05)。 季风季节对精液质量的影响更大,液态精液储存48小时后仍可使用。
{"title":"Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands","authors":"P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar","doi":"10.4103/2305-0500.390304","DOIUrl":"https://doi.org/10.4103/2305-0500.390304","url":null,"abstract":"\u0000 \u0000 To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons.\u0000 \u0000 \u0000 \u0000 A total of 10 Teressa bucks (3-4 years) were selected from breeding farm, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India. Semen samples (n=25 per season) were collected through artificial vagina method and preserved at refrigerated temperature (5°C) for 48 h using Tris citrate glucose based extender. We detected semen quality parameters [volume, mass activity, pH, sperm concentration, total motility, viability, total sperm abnormality, and plasma membrane, acrosomal and nuclear integrities], biochemical profiles [aspartate amino transferase (AST), alanine amino transferase (ALT) and total cholesterol], and oxidative stress markers [total antioxidant capacity (TAC) and malondialdehyde (MDA)] during monsoon and dry seasons.\u0000 \u0000 \u0000 \u0000 Semen quality parameters significantly differed between seasons (P<0.05) and among storage periods (P<0.05). Volume, pH, mass activity, motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly higher (P<0.05). Sperm concentration, sperm abnormalities, MDA, AST, ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season (P<0.05). Motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly decreased (P<0.05) while sperm abnormality, AST, ALT, total cholesterol and MDA were significantly increased as liquid semen storage period advanced (P<0.05).\u0000 \u0000 \u0000 \u0000 Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h. Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"199 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139013770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of VEGF165 and VEGF165b during ovarian follicular development 卵巢卵泡发育过程中 VEGF165 和 VEGF165b 的表达
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390303
Chinnarat Changsangfa, Bongkoch Turathum, Morakot Sroyraya, Khwanthana Grataitong
To investigate the role of vascular endothelial growth factor (VEGF)165a, VEGF165b, and VEGF receptor (VEGFR) in the development of bovine follicles. We cultured follicular cells that were collected from small, medium, and large sized bovine follicles with estrogen and measured the expression of VEGF, VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence. The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium. The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h. VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h. In addition, VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h. This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner. This reveals an advantage during oocyte maturation in vitro.
研究血管内皮生长因子(VEGF)165a、VEGF165b 和血管内皮生长因子受体(VEGFR)在牛卵泡发育过程中的作用。 我们用雌激素培养了从小号、中号和大号牛卵泡中收集的卵泡细胞,并通过 Western 印迹分析和免疫荧光法测定了 VEGF、VEGFR2 和 VEGF165b 的表达。 在含有雌激素的培养基中培养后,所有大小的卵泡中 VEGF165 的表达量都有所增加,小卵泡和大卵泡中 VEGF165b 的表达量也有所增加。这些证据表明,在牛卵泡发育过程中,VEGF165 和 VEGFR 的表达与雌激素刺激有关,并且是以自分泌或旁分泌的方式表达的。这揭示了体外卵母细胞成熟过程中的优势。
{"title":"Expression of VEGF165 and VEGF165b during ovarian follicular development","authors":"Chinnarat Changsangfa, Bongkoch Turathum, Morakot Sroyraya, Khwanthana Grataitong","doi":"10.4103/2305-0500.390303","DOIUrl":"https://doi.org/10.4103/2305-0500.390303","url":null,"abstract":"\u0000 \u0000 To investigate the role of vascular endothelial growth factor (VEGF)165a, VEGF165b, and VEGF receptor (VEGFR) in the development of bovine follicles.\u0000 \u0000 \u0000 \u0000 We cultured follicular cells that were collected from small, medium, and large sized bovine follicles with estrogen and measured the expression of VEGF, VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence.\u0000 \u0000 \u0000 \u0000 The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium. The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h. VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h. In addition, VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h.\u0000 \u0000 \u0000 \u0000 This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner. This reveals an advantage during oocyte maturation in vitro.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"113 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidative stress in male infertility and therapeutic approach: A mini-review 男性不育症中的氧化应激与治疗方法:微型综述
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390299
Marwa Lahimer, H. Mustapha, Véronique Bach, H. Khorsi-Cauet, Moncef Benkhalifa, Mounir Ajina, Habib Ben Ali
Growing evidence suggests that oxidative stress is a major cause of male infertility. Spermatozoa are highly sensitive cells due to the vulnerability of their membrane fatty acids and the diminished antioxidant defense. Reactive oxygen species (ROS) impact multiple signaling pathways involved in mitochondrial dysfunction, hormonal unbalance and semen quality decline. The origin of ROS is diverse, including generated normal cellular metabolism, environmental exposure, advanced paternal age and inflammation. Research has indicated that antioxidant supplementation can improve oxidative stress and reduce the risk of chronic diseases. Moreover, it is widely known that antioxidant supplementation can enhance sperm quality and increase the pregnancy rate in couples undergoing fertility treatments. The current study highlights the deleterious effect of ROS and its impact on semen function. In addition, it contributes to the development of a clinical approach for the use of antioxidants in the management of male infertility.
越来越多的证据表明,氧化应激是导致男性不育的主要原因。由于精子膜脂肪酸的脆弱性和抗氧化防御能力的减弱,精子是高度敏感的细胞。活性氧(ROS)会影响多种信号通路,导致线粒体功能障碍、荷尔蒙失衡和精液质量下降。ROS 的来源多种多样,包括正常的细胞新陈代谢、环境暴露、高龄父亲和炎症。研究表明,补充抗氧化剂可以改善氧化应激,降低慢性疾病风险。此外,众所周知,补充抗氧化剂可以提高精子质量,增加接受生育治疗的夫妇的受孕率。本研究强调了 ROS 的有害作用及其对精液功能的影响。此外,这项研究还有助于开发一种临床方法,用于治疗男性不育症。
{"title":"Oxidative stress in male infertility and therapeutic approach: A mini-review","authors":"Marwa Lahimer, H. Mustapha, Véronique Bach, H. Khorsi-Cauet, Moncef Benkhalifa, Mounir Ajina, Habib Ben Ali","doi":"10.4103/2305-0500.390299","DOIUrl":"https://doi.org/10.4103/2305-0500.390299","url":null,"abstract":"Growing evidence suggests that oxidative stress is a major cause of male infertility. Spermatozoa are highly sensitive cells due to the vulnerability of their membrane fatty acids and the diminished antioxidant defense. Reactive oxygen species (ROS) impact multiple signaling pathways involved in mitochondrial dysfunction, hormonal unbalance and semen quality decline. The origin of ROS is diverse, including generated normal cellular metabolism, environmental exposure, advanced paternal age and inflammation. Research has indicated that antioxidant supplementation can improve oxidative stress and reduce the risk of chronic diseases. Moreover, it is widely known that antioxidant supplementation can enhance sperm quality and increase the pregnancy rate in couples undergoing fertility treatments. The current study highlights the deleterious effect of ROS and its impact on semen function. In addition, it contributes to the development of a clinical approach for the use of antioxidants in the management of male infertility.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"279 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plantain-based diet modulates atrazine-induced testicular toxicities in rats 以大车前草为基础的饮食调节阿特拉津诱导的大鼠睾丸毒性
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380984
D. Rotimi, O. Adeyemi
Objective: To assess the potential of plantain-based diet in modulating testicular toxicities in rats exposed to atrazine. Methods: The plantain-based diet at 50%, 25% and 12.5% were prepared from the basal diet by substituting the corn starch with plantain fruit pulp flour at different percentages. Wistar rats were fed plantain-based diet in varying concentrations ranging from 12.5% to 50% of the basal diet for 21 days before or after atrazine treatment in a two-phase experiment: preventive and therapeutic phases. The therapeutic model (n=35) had seven groups with 5 rats each, including the control, atrazine, atrazine recovery, atrazine plus plantain-based diet 50%, 25%, 12.5%, and atrazine plus quercetin groups. The preventive model (n=30) had six groups of 5 rats, consisting of the control, atrazine, 50%, 25%, 12.5% plantain-based diet plus atrazine, and quercetin plus atrazine groups. Gonadal hormones (testosterone, luteinizing hormone and follicle-stimulating hormone), sperm parameters (sperm motility, viability, morphology and concentration), and testicular function indices (protein, cholesterol, glycogen, acid phosphatase, alkaline phosphatase and lactate dehydrogenase) were measured. Results: The gonadal hormones, sperm characteristics, and testicular function indices of the rat testis decreased significantly in the atrazine group alongside degeneration of the histoarchitecture. However, plantain-based diet restored the gonadal hormone concentrations, semen parameters, and testicular function indices in both the preventive and therapeutic models. Conclusions: Treatment with plantain-based diet protects against rat testicular toxicity caused by atrazine via the modulation of gonadal hormones, sperm quality, testicular function index as well as histoarchitecture of rat testes.
目的:评价车前草为基础的饮食对阿特拉津暴露大鼠睾丸毒性的调节作用。方法:在基础饲粮的基础上,以不同比例的车前草果肉粉代替玉米淀粉,分别配制50%、25%和12.5%车前草基础饲粮。在阿特拉津治疗前后的21天内,Wistar大鼠被喂食以车前草为基础的饲料,其浓度在基础饲料的12.5%至50%之间,分为两阶段:预防和治疗阶段。治疗模型(n=35)分为7组,每组5只,包括对照组、阿特拉津组、阿特拉津恢复组、阿特拉津加车前草饲粮50%组、25%组、12.5%组和阿特拉津加槲皮素组。预防模型(n=30)设6组,每组5只,分为对照组、阿特拉津组、50%、25%、12.5%车前草饲粮加阿特拉津组和槲皮素加阿特拉津组。测定性腺激素(睾酮、黄体生成素和促卵泡激素)、精子参数(精子活力、活力、形态和浓度)和睾丸功能指标(蛋白质、胆固醇、糖原、酸性磷酸酶、碱性磷酸酶和乳酸脱氢酶)。结果:阿特拉津组大鼠睾丸性腺激素、精子特征和睾丸功能指标明显降低,组织结构变性。然而,以大车前草为基础的饮食在预防和治疗模型中均能恢复性激素浓度、精液参数和睾丸功能指数。结论:车前草饲粮可通过调节性激素、精子质量、睾丸功能指数和睾丸组织结构来预防阿特拉津所致大鼠睾丸毒性。
{"title":"Plantain-based diet modulates atrazine-induced testicular toxicities in rats","authors":"D. Rotimi, O. Adeyemi","doi":"10.4103/2305-0500.380984","DOIUrl":"https://doi.org/10.4103/2305-0500.380984","url":null,"abstract":"Objective: To assess the potential of plantain-based diet in modulating testicular toxicities in rats exposed to atrazine. Methods: The plantain-based diet at 50%, 25% and 12.5% were prepared from the basal diet by substituting the corn starch with plantain fruit pulp flour at different percentages. Wistar rats were fed plantain-based diet in varying concentrations ranging from 12.5% to 50% of the basal diet for 21 days before or after atrazine treatment in a two-phase experiment: preventive and therapeutic phases. The therapeutic model (n=35) had seven groups with 5 rats each, including the control, atrazine, atrazine recovery, atrazine plus plantain-based diet 50%, 25%, 12.5%, and atrazine plus quercetin groups. The preventive model (n=30) had six groups of 5 rats, consisting of the control, atrazine, 50%, 25%, 12.5% plantain-based diet plus atrazine, and quercetin plus atrazine groups. Gonadal hormones (testosterone, luteinizing hormone and follicle-stimulating hormone), sperm parameters (sperm motility, viability, morphology and concentration), and testicular function indices (protein, cholesterol, glycogen, acid phosphatase, alkaline phosphatase and lactate dehydrogenase) were measured. Results: The gonadal hormones, sperm characteristics, and testicular function indices of the rat testis decreased significantly in the atrazine group alongside degeneration of the histoarchitecture. However, plantain-based diet restored the gonadal hormone concentrations, semen parameters, and testicular function indices in both the preventive and therapeutic models. Conclusions: Treatment with plantain-based diet protects against rat testicular toxicity caused by atrazine via the modulation of gonadal hormones, sperm quality, testicular function index as well as histoarchitecture of rat testes.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"170 - 181"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47665593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pregnancy outcomes following supplementation of single dose GnRH agonist to sustain the luteal phase in antagonist fresh embryo transfer cycles: A multicentric prospective cohort study 在拮抗剂新鲜胚胎移植周期中补充单剂量GnRH激动剂以维持黄体期后的妊娠结局:一项多中心前瞻性队列研究
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380983
Tatapudi Parvathi Devi, A. Gahlot, Sangita Sharma, M. Choudhary, R. Soni, Meeta Sharma
Objective: To determine whether a single dose of gonadotropin-releasing hormone (GnRH) agonist administered subcutaneously in addition to the regular progesterone supplementation could provide a better luteal support in antagonist protocol fresh embryo transfer cycles. Methods: This prospective, multicentric, cohort study included total 140 women, 70 in each group. Controlled ovarian stimulation was carried out as per fixed GnRH antagonist protocol. The trigger was given with hCG. In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) was performed and day-3 embryos were transferred. Patients were divided into groups 1 and 2 based on computer generated randomization sheet. Six days following oocyte retrieval, group 1 received 0.2 mg decapeptyl subcutaneously in addition to regular progesterone support while group 2 received progesterone only. Luteal support was given for 14 days to both groups; if pregnancy was confirmed luteal support was continued till 12 weeks of gestation. The clinical pregnancy rate was the primary outcome. The implantation rate, miscarriage rate, live birth delivery rate, and multiple pregnancy rates were the secondary outcomes. Results: A total of 140 patients were analysed, 70 in each group. Clinical pregnancy rates (47.1% vs. 35.7%; P=0.17), implantation rates (23.4% vs. 18.1%, P=0.24), live birth delivery rates (41.4% vs. 27.1%, P=0.08), and multiple pregnancy rates (21.2% vs. 16.0%, P=0.74) were higher in group 1 than in group 2. Group 1 had a lower miscarriage rate than group 2 (5.7% vs. 8.6%; P=0.75). However, these differences were not statistically significant between the two groups. Conclusions: Administration of a single dose of GnRH agonist in addition to regular natural micronized vaginal progesterone as luteal support in GnRH antagonist protocol cycles marginally improves implantation rates, clinical pregnancy rates, and live birth delivery rates. However, more studies with higher sample sizes are needed before any conclusive statements about GnRH agonist as luteal phase support can be made.
目的:探讨单剂量促性腺激素释放激素(GnRH)激动剂在常规黄体酮补充的基础上皮下注射是否能在拮抗剂方案的新鲜胚胎移植周期中提供更好的黄体支持。方法:这项前瞻性、多中心、队列研究共纳入140名女性,每组70名。对照卵巢刺激按照固定GnRH拮抗剂方案进行。触发剂是hCG。进行体外受精/胞浆内单精子注射(IVF/ICSI),移植第3天的胚胎。根据计算机生成的随机分组表将患者分为1组和2组。取卵6天后,组1在常规黄体酮支持的基础上皮下注射十肽0.2 mg,组2只注射黄体酮。两组均给予黄体支持14 d;如果确认怀孕,黄体支持持续到妊娠12周。临床妊娠率为主要观察指标。次要指标为着床率、流产率、活产率和多胎妊娠率。结果:共分析140例患者,每组70例。临床妊娠率(47.1% vs. 35.7%;P=0.17)、着床率(23.4% vs. 18.1%, P=0.24)、活产率(41.4% vs. 27.1%, P=0.08)、多胎妊娠率(21.2% vs. 16.0%, P=0.74)均高于组2。1组流产率低于2组(5.7%比8.6%;P = 0.75)。然而,这些差异在两组之间没有统计学意义。结论:在GnRH拮抗剂方案周期中,单剂量GnRH激动剂加常规天然微粉阴道孕酮作为黄体支持剂,可略微提高着床率、临床妊娠率和活产率。然而,在任何关于GnRH激动剂作为黄体期支持剂的结论性声明之前,需要更多样本量更大的研究。
{"title":"Pregnancy outcomes following supplementation of single dose GnRH agonist to sustain the luteal phase in antagonist fresh embryo transfer cycles: A multicentric prospective cohort study","authors":"Tatapudi Parvathi Devi, A. Gahlot, Sangita Sharma, M. Choudhary, R. Soni, Meeta Sharma","doi":"10.4103/2305-0500.380983","DOIUrl":"https://doi.org/10.4103/2305-0500.380983","url":null,"abstract":"Objective: To determine whether a single dose of gonadotropin-releasing hormone (GnRH) agonist administered subcutaneously in addition to the regular progesterone supplementation could provide a better luteal support in antagonist protocol fresh embryo transfer cycles. Methods: This prospective, multicentric, cohort study included total 140 women, 70 in each group. Controlled ovarian stimulation was carried out as per fixed GnRH antagonist protocol. The trigger was given with hCG. In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) was performed and day-3 embryos were transferred. Patients were divided into groups 1 and 2 based on computer generated randomization sheet. Six days following oocyte retrieval, group 1 received 0.2 mg decapeptyl subcutaneously in addition to regular progesterone support while group 2 received progesterone only. Luteal support was given for 14 days to both groups; if pregnancy was confirmed luteal support was continued till 12 weeks of gestation. The clinical pregnancy rate was the primary outcome. The implantation rate, miscarriage rate, live birth delivery rate, and multiple pregnancy rates were the secondary outcomes. Results: A total of 140 patients were analysed, 70 in each group. Clinical pregnancy rates (47.1% vs. 35.7%; P=0.17), implantation rates (23.4% vs. 18.1%, P=0.24), live birth delivery rates (41.4% vs. 27.1%, P=0.08), and multiple pregnancy rates (21.2% vs. 16.0%, P=0.74) were higher in group 1 than in group 2. Group 1 had a lower miscarriage rate than group 2 (5.7% vs. 8.6%; P=0.75). However, these differences were not statistically significant between the two groups. Conclusions: Administration of a single dose of GnRH agonist in addition to regular natural micronized vaginal progesterone as luteal support in GnRH antagonist protocol cycles marginally improves implantation rates, clinical pregnancy rates, and live birth delivery rates. However, more studies with higher sample sizes are needed before any conclusive statements about GnRH agonist as luteal phase support can be made.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"162 - 169"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48233969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of the ovine ovarian reserve during the prenatal and neonatal periods 产前和新生儿期绵羊卵巢储备的测定
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380986
Isam B. Sharum
Objective: To determine the ovine ovarian histomorphology and follicular staging at various age periods in Awassi breed. Methods: Ovaries were collected from prenatal fetuses [gestational age (95±5) days], neonatal (day 0), and prepubertal ewe lambs (two and four months of age); each age group included six animals. Ovaries (n=12, each group) were dissected and processed for hematoxylin and eosin staining. Stained sections (n=24, each group) were imaged and utilized for histomorphology assessment, follicle measurement, and classification. Results: Prenatal ovaries were mainly enriched with primordial follicles accompanied by a lower proportion of primary follicles. In addition to primordial and primary follicles, neonatal ovaries demonstrated a proportion of centrally located multilayered and antral follicles. In comparison with neonatal ovaries, the proportion of multilayered and antral follicles was significantly higher in the ovaries of two-month-old lambs; conversely, the proportion of peripherally situated primordial follicles dramatically declined compared to that of earlier age of lamb. Although there was no statistical variation in the sizes of primordial follicles across groups, the mean diameter of the primary follicle in the prenatal ovaries was substantially smaller than in postnatal ovaries. Compared to the neonatal ovaries, the size of the multilayered and antral follicles in the prepubertal ovaries was substantially larger. Conclusions: The earliest follicular developmental stages were established prenatally whereas the advanced growth stages started in the neonatal period and greatly increased in the prepubertal period.
目的:了解Awassi不同年龄段绵羊卵巢组织形态及卵泡分期。方法:从产前胎儿[胎龄(95±5)天]、新生儿(0天)和青春期前母羊羔羊(2个月龄和4个月龄)采集卵巢;每个年龄组包括6只动物。解剖卵巢(每组n=12)并进行苏木精和伊红染色。对染色切片(每组n=24)进行成像,并用于组织形态学评估、毛囊测量和分类。结果:产前卵巢主要富含原始卵泡,初级卵泡比例较低。除了原始卵泡和初级卵泡外,新生儿卵巢还显示出一定比例的位于中心的多层和窦状卵泡。与新生儿卵巢相比,两个月大的羔羊卵巢中多层卵泡和窦状卵泡的比例明显更高;相反,与早期羔羊相比,位于外围的原始卵泡的比例显著下降。尽管各组原始卵泡的大小没有统计学差异,但产前卵巢中初级卵泡的平均直径明显小于产后卵巢。与新生儿卵巢相比,青春期前卵巢中多层和窦状卵泡的大小要大得多。结论:最早的卵泡发育阶段是在产前建立的,而晚期生长阶段始于新生儿期,并在青春期前大大增加。
{"title":"Determination of the ovine ovarian reserve during the prenatal and neonatal periods","authors":"Isam B. Sharum","doi":"10.4103/2305-0500.380986","DOIUrl":"https://doi.org/10.4103/2305-0500.380986","url":null,"abstract":"Objective: To determine the ovine ovarian histomorphology and follicular staging at various age periods in Awassi breed. Methods: Ovaries were collected from prenatal fetuses [gestational age (95±5) days], neonatal (day 0), and prepubertal ewe lambs (two and four months of age); each age group included six animals. Ovaries (n=12, each group) were dissected and processed for hematoxylin and eosin staining. Stained sections (n=24, each group) were imaged and utilized for histomorphology assessment, follicle measurement, and classification. Results: Prenatal ovaries were mainly enriched with primordial follicles accompanied by a lower proportion of primary follicles. In addition to primordial and primary follicles, neonatal ovaries demonstrated a proportion of centrally located multilayered and antral follicles. In comparison with neonatal ovaries, the proportion of multilayered and antral follicles was significantly higher in the ovaries of two-month-old lambs; conversely, the proportion of peripherally situated primordial follicles dramatically declined compared to that of earlier age of lamb. Although there was no statistical variation in the sizes of primordial follicles across groups, the mean diameter of the primary follicle in the prenatal ovaries was substantially smaller than in postnatal ovaries. Compared to the neonatal ovaries, the size of the multilayered and antral follicles in the prepubertal ovaries was substantially larger. Conclusions: The earliest follicular developmental stages were established prenatally whereas the advanced growth stages started in the neonatal period and greatly increased in the prepubertal period.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"194 - 200"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48731045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The barriers of joining in vitro fertilization programs among infertile couples in developing countries: A scoping review 发展中国家不孕夫妇参与体外受精项目的障碍:范围界定综述
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380981
A. Dewi, A. Wicaksana, Muhammad Lutfi, Agung Dewanto
Objective: To determine the barriers of joining in-vitro fertilization (IVF) programs among infertile couples in developing countries. Methods: This study assessed infertile couples and the barriers or associated factors resulting in delayed decision-making of joining IVF program by searching databases PubMed, ScienceDirect, EBSCO, ClinicalKey, and Cochrane Library from inception until December 31, 2021. Additional search strategies were snowballing literature search and citation tracking. Results: Eleven articles were included in the scoping review. The cost was the greatest barrier of joining IVF program. Limited access and lack of assisted reproductive technology centers, few qualified infertility trained staff, insufficient government support, low priority in government policy, along with sociocultural factors, such as religion and false beliefs or myths were also majorly considered to be associated obstacles. Conclusions: The main barrier associated with IVF program among infertile couples in developing countries is the high cost of the IVF services.
目的:确定发展中国家不孕夫妇参加体外受精(IVF)计划的障碍。方法:本研究通过检索PubMed、ScienceDirect、EBSCO、ClinicalKey和Cochrane Library数据库,从研究开始到2021年12月31日,评估不孕夫妇以及导致延迟加入IVF计划决策的障碍或相关因素。其他的搜索策略是滚雪球式的文献搜索和引文跟踪。结果:11篇文章被纳入范围审查。费用是加入体外受精计划的最大障碍。辅助生殖技术中心有限和缺乏,合格的不孕症培训人员很少,政府支持不足,政府政策不优先,以及宗教和错误信仰或神话等社会文化因素,也被认为是主要的相关障碍。结论:发展中国家不孕夫妇进行体外受精的主要障碍是体外受精服务的高成本。
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引用次数: 0
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Asian Pacific Journal of Reproduction
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