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Efficacy of intraovarian autologous platelet-rich plasma administration in women with low ovarian reserve: A systematic review and meta-analysis 卵巢内注射自体富血小板血浆对卵巢储备功能低下妇女的疗效:系统回顾和荟萃分析
Q1 Veterinary Pub Date : 2024-03-01 DOI: 10.4103/apjr.apjr_141_23
Yasmin Sabina Sa’diah, Agung Dewanto, L. A. Chandra
To conduct a systematic examination and meta-analysis of the most reliable data from experimental studies evaluating the efficacy of autologous platelet-rich plasma (PRP) on low ovarian reserve. A comprehensive search was performed utilizing pertinent search terms across electronic databases, including PubMed, Cochrane, and Google Scholar. We included studies that assigned infertile women with low ovarian reserve in experimental studies. Ovarian reserve parameters were measured before and after PRP injection into ovaries. The data of each study was retrieved and subsequently compiled. Of 301 articles collected and reviewed, six studies were finally included in the meta-analysis. Following PRP injection, infertile women showed a non-significant increase in anti-Mullerian hormone (AMH) level (MD=0.10; 95% CI -0.04, 0.23), a significant increase in antral follicular count (AFC) (MD=1.88; 95% CI 0.47, 3.29), and a non-significant reduction in follicle-stimulating hormone (FSH) level (MD=-0.22; 95% CI -8.32, 7.87). Autologous PRP may increase AFC, but not AMH. Although it is found beneficial in enhancing ovarian reserve (AFC), further research with strong evidence is still required.
对评估自体富血小板血浆(PRP)对卵巢储备功能低下疗效的实验研究中最可靠的数据进行系统检查和荟萃分析。 我们利用相关检索词在PubMed、Cochrane和谷歌学术等电子数据库中进行了全面检索。我们纳入了将卵巢储备功能低下的不孕妇女分配到实验研究中的研究。在向卵巢注射 PRP 之前和之后测量了卵巢储备参数。我们检索了每项研究的数据,随后进行了汇编。 在收集和审查的 301 篇文章中,最终有 6 项研究被纳入荟萃分析。注射 PRP 后,不孕妇女的抗苗勒氏管激素(AMH)水平出现了非显著性升高(MD=0.10;95% CI -0.04,0.23),前卵泡计数(AFC)显著升高(MD=1.88;95% CI 0.47,3.29),而促卵泡激素(FSH)水平则出现了非显著性降低(MD=-0.22;95% CI -8.32,7.87)。 自体 PRP 可增加 AFC,但不会增加 AMH。虽然自体血浆蛋白对提高卵巢储备功能(AFC)有益,但仍需要进一步的研究和有力的证据。
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引用次数: 0
Semen features for conventional IVF: Need for a simple universal index 常规试管婴儿的精液特征:需要一个简单的通用指标
Q1 Veterinary Pub Date : 2024-03-01 DOI: 10.4103/apjr.apjr_152_23
M. Işıkoğlu
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引用次数: 0
Adiponectin orchestrates testosterone suppression in biological pathways 脂肪连通素在生物通路中协调睾酮抑制作用
Q1 Veterinary Pub Date : 2024-03-01 DOI: 10.4103/apjr.apjr_162_23
Ekhoye Ehitare Ikekhuamen, Imonna Kingsle
This current review highlights adiponectin engagement with AdipoRl and AdipoR2 which subsequently triggers pathways such as AMPK, PPARα, and MAPK, thereby modulating testicular steroidogenesis. Adiponectin's actions on Leydig and adrenal cells inhibit androgen secretion by suppressing the steroidogenic acute regulatory protein (StAR). Given that StAR facilitates cholesterol to testosterone conversion, AMPK inhibits this process by modulating cholesterol transport and suppressing StAR expression through multiple avenues. Furthermore, adiponectin-induced PPARα activation impedes mitochondrial cholesterol influx, further modulating androgen biosynthesis. The suppressive influence of PPARα on steroidogenic genes, notably StAR, is evident. Collectively, adiponectin signalling predominantly attenuates androgen production, ensuring metabolic and reproductive equilibrium. Imbalances, as seen in conditions like hypogonadism and obesity-related infertility, highlight their crucial roles and potential clinical interventions for reproductive disorders.
本综述重点介绍了脂肪连通素与 AdipoRl 和 AdipoR2 的相互作用,随后触发 AMPK、PPARα 和 MAPK 等通路,从而调节睾丸的类固醇生成。Adiponectin 对 Leydig 和肾上腺细胞的作用是通过抑制类固醇生成急性调节蛋白(StAR)来抑制雄激素分泌。鉴于 StAR 可促进胆固醇向睾酮的转化,AMPK 可通过多种途径调节胆固醇转运并抑制 StAR 的表达,从而抑制这一过程。此外,脂联素诱导的 PPARα 激活会阻碍线粒体胆固醇的流入,从而进一步调节雄激素的生物合成。PPARα 对类固醇生成基因(尤其是 StAR)的抑制作用显而易见。总之,脂肪连通素信号主要抑制雄激素的产生,确保新陈代谢和生殖平衡。性腺功能减退症和肥胖相关性不孕症等疾病中出现的失衡现象凸显了它们的关键作用以及对生殖障碍的潜在临床干预。
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引用次数: 0
Effect of Moringa oleifera on semen quality profiles in endangered Teressa goat under tropical humid island ecosystem 辣木对热带潮湿岛屿生态系统下濒危泰瑞莎山羊精液质量的影响
Q1 Veterinary Pub Date : 2024-03-01 DOI: 10.4103/apjr.apjr_159_23
P. Perumal, J. Sunder, A. De, A. Nahak, D. Chaurasia, T. C. Balamurugan, D. Bhattacharya
To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck. A total of 25 semen samples from six bucks were selected for the study. Spermatozoa of 150× 106 were incubated in 0, 300, 500 and 700 μg of leaf extract as group I, II, III and IV, respectively. Liquid stored semen samples were analysed for motility, viability, total sperm abnormalities, plasma membrane, acrosomal and nuclear integrities, seminal plasma intracellular enzymes [aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH)] and total antioxidant capacity (TAC) and spermatozoa malondialdehyde (MDA) and cholesterol efflux in comparison with the control group (group I) for up to 72 h. Moringa oleifera leaf extract (500 μg/150× 106 spermatozoa)-treated semen had significantly higher motility, viability, plasma membrane, acrosomal and nuclear integrities, and TAC (P<0.05), and had significantly lower total sperm abnormalities, AST, ALT, LDH, MDA production and cholesterol efflux compared to those in other Moringa oleifera leaf extract treated (300 or 700 μg/150×106 spermatozoa) and control groups at different hours of liquid storage (P<0.05). Semen quality parameters and TAC showed an increasing trend and total sperm abnormalities, MDA production, leakage of intra-cellular enzymes and cholesterol efflux showed a decreasing trend from group I to group III and then an opposite trend from group II to group IV at different hours of liquid storage. Moringa oleifera leaf extract 500 μg/150×106 spermatozoa can be used for liquid semen preservation in Teressa goat at Andaman and Nicobar Islands.
研究辣木叶提取物对泰瑞莎山羊雄鹿精液质量参数的影响。 研究共选取了六头公山羊的 25 份精液样本。将 150×106 的精子分别放入 0、300、500 和 700 μg 的叶提取物中培养,分为 I、II、III 和 IV 组。对液态储存精液样本的运动性、存活率、精子总畸形率、质膜、顶体和核完整性、精浆细胞内酶[天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(AST)]进行分析、总抗氧化能力(TAC)、精子丙二醛(MDA)和胆固醇外流。 经油辣木叶提取物(500 μg/150× 106 个精子)处理的精液的运动能力、存活率、质膜、顶体和核完整性以及 TAC 均显著提高(P<0.05),与其他油杉叶提取物处理组(300 或 700 μg/150×106 精子)和对照组相比,在不同的液体贮存时间内,精子总畸形率、AST、ALT、LDH、MDA 生成量和胆固醇外流均明显降低(P<0.05)。精液质量指标和 TAC 呈上升趋势,而精子总畸形率、MDA 生成量、细胞内酶渗漏和胆固醇外流呈下降趋势,从第一组到第三组,然后从第二组到第四组,在不同的液体储存时间内呈相反趋势。 油辣木叶提取物 500 μg/150×106 精子可用于安达曼和尼科巴群岛 Teressa 山羊的精液保存。
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引用次数: 0
Covid-19 vaccination in pregnancy and placental pathology 孕期接种 Covid-19 疫苗与胎盘病理学
Q1 Veterinary Pub Date : 2024-03-01 DOI: 10.4103/apjr.apjr_94_23
Leo Simanjuntak
To describe and determine the association between Covid-19 vaccination in pregnancy and placental pathology. Conducted in a tertiary hospital in Medan, Indonesia, from April 30th 2022 to June 30th 2022, this single-center cross-sectional study involved Covid-19 vaccinated and unvaccinated women with singleton full-term pregnancies delivering live fetuses via cesarean section. Maternal characteristics, placental pathologies, and the placental index were documented at enrollment. The association between Covid-19 vaccination status and placental pathology was assessed. The study enrolled 200 pregnant women, including 110 vaccinated women and 90 unvaccinated women. No significant differences were observed in birthweight (P=0.48), placental index (P=0.48), and placental pathology findings [intervillous bleeding (P=0.20), increased syncytial knots (P=0.83), chorangiosis (P=0.13), villous stromal edema (P=0.13), vascular dilation and congestion (P=0.13), and vascular wall thrombus (P=0.71)] between the vaccinated and unvaccinated groups. This study revealed no statistically significant association between Covid-19 vaccination and placental pathology. The findings support the safety of Covid-19 vaccination during pregnancy, in regards to changes of the placental pathology.
描述并确定孕期接种Covid-19疫苗与胎盘病理学之间的关联。 这项单中心横断面研究于 2022 年 4 月 30 日至 2022 年 6 月 30 日在印度尼西亚棉兰的一家三甲医院进行,涉及接种和未接种 Covid-19 疫苗的单胎足月妊娠妇女,她们均通过剖腹产分娩活胎。研究人员在入组时记录了产妇特征、胎盘病变和胎盘指数。评估了 Covid-19 疫苗接种情况与胎盘病理学之间的关联。 该研究共招募了 200 名孕妇,包括 110 名接种过疫苗的孕妇和 90 名未接种疫苗的孕妇。在接种组和未接种组之间,出生体重(P=0.48)、胎盘指数(P=0.48)和胎盘病理结果[绒毛间出血(P=0.20)、合胞结增加(P=0.83)、脉管扩张(P=0.13)、绒毛基质水肿(P=0.13)、血管扩张和充血(P=0.13)以及血管壁血栓(P=0.71)]均无明显差异。 本研究表明,接种 Covid-19 疫苗与胎盘病理学之间没有统计学意义上的显著关联。研究结果支持孕期接种 Covid-19 疫苗在胎盘病理变化方面的安全性。
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引用次数: 0
Protective effect of co-enzyme Q10 on testicular tissue and sperm parameters in adult male rats treated with Sunset Yellow FCF 辅酶 Q10 对使用日落黄 FCF 的成年雄性大鼠睾丸组织和精子参数的保护作用
Q1 Veterinary Pub Date : 2024-03-01 DOI: 10.4103/apjr.apjr_55_23
Rohollah Nazari, Somayeh Akbari, Maryam Naseh, Shayan Yousufzai, S. F. Hosseini, Fatemeh Karimi
To determine the protective effect of co-enzyme Q10 (CoQ10) on testicular tissue and sperm parameters in male rats treated with Sunset Yellow FCF. Sixty male Sprague-Dawley rats were randomly divided into 6 groups of the control, CoQ10 (10 mg/kg/day), low dose of Sunset Yellow (2.5 mg/kg), high dose of Sunset Yellow (70 mg/kg), low dose of Sunset Yellow (2.5 mg/kg) plus CoQ10, and high dose of Sunset Yellow (70 mg/kg) plus CoQ10. The drugs were administered via daily oral gavages for 6 weeks. At the end of the experiment, sperm analysis, stereological and histological assessments of the testis were carried out. The normal morphology (by 41.1%) and progressive spermatozoa (by 74.8%), testicle volume (by 33.4%), lumen volume (by 38.3%), interstitial tissue volume (by 44.7%), seminiferous tubule volume (by 40.7%), and number of spermatogonia (by 53.9%) and Leydig cells (by 70.7%) reduced in the rats that received high doses of Sunset Yellow in comparison to the control group. Nonetheless, all these alterations were recovered by CoQ10 treatment in the CoQ10 plus high dose of Sunset Yellow group. Furthermore, low doses of Sunset Yellow did not affect different parameters of the testis and sperm. CoQ10 could, to some extent, prevent structural changes of the testis induced by the high dose of Sunset Yellow.
研究辅酶Q10(CoQ10)对使用日落黄FCF的雄性大鼠睾丸组织和精子参数的保护作用。 将 60 只雄性 Sprague-Dawley 大鼠随机分为 6 组,分别为对照组、辅酶 Q10 组(10 毫克/千克/天)、低剂量日落黄组(2.5 毫克/千克)、高剂量日落黄组(70 毫克/千克)、低剂量日落黄组(2.5 毫克/千克)加辅酶 Q10 组和高剂量日落黄组(70 毫克/千克)加辅酶 Q10 组。这些药物通过每日口服给药,持续 6 周。实验结束后,对睾丸进行了精子分析、立体学和组织学评估。 与对照组相比,接受高剂量夕阳黄治疗的大鼠睾丸正常形态(减少 41.1%)、精子数量(减少 74.8%)、睾丸体积(减少 33.4%)、管腔体积(减少 38.3%)、间质组织体积(减少 44.7%)、曲细精管体积(减少 40.7%)、精原细胞数量(减少 53.9%)和 Leydig 细胞数量(减少 70.7%)均有所减少。然而,在辅酶Q10加高浓度日落黄组中,所有这些变化都通过辅酶Q10治疗得以恢复。此外,低剂量的日落黄并未影响睾丸和精子的不同参数。 辅酶Q10可在一定程度上防止高剂量日落黄引起的睾丸结构变化。
{"title":"Protective effect of co-enzyme Q10 on testicular tissue and sperm parameters in adult male rats treated with Sunset Yellow FCF","authors":"Rohollah Nazari, Somayeh Akbari, Maryam Naseh, Shayan Yousufzai, S. F. Hosseini, Fatemeh Karimi","doi":"10.4103/apjr.apjr_55_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_55_23","url":null,"abstract":"\u0000 \u0000 To determine the protective effect of co-enzyme Q10 (CoQ10) on testicular tissue and sperm parameters in male rats treated with Sunset Yellow FCF.\u0000 \u0000 \u0000 \u0000 Sixty male Sprague-Dawley rats were randomly divided into 6 groups of the control, CoQ10 (10 mg/kg/day), low dose of Sunset Yellow (2.5 mg/kg), high dose of Sunset Yellow (70 mg/kg), low dose of Sunset Yellow (2.5 mg/kg) plus CoQ10, and high dose of Sunset Yellow (70 mg/kg) plus CoQ10. The drugs were administered via daily oral gavages for 6 weeks. At the end of the experiment, sperm analysis, stereological and histological assessments of the testis were carried out.\u0000 \u0000 \u0000 \u0000 The normal morphology (by 41.1%) and progressive spermatozoa (by 74.8%), testicle volume (by 33.4%), lumen volume (by 38.3%), interstitial tissue volume (by 44.7%), seminiferous tubule volume (by 40.7%), and number of spermatogonia (by 53.9%) and Leydig cells (by 70.7%) reduced in the rats that received high doses of Sunset Yellow in comparison to the control group. Nonetheless, all these alterations were recovered by CoQ10 treatment in the CoQ10 plus high dose of Sunset Yellow group. Furthermore, low doses of Sunset Yellow did not affect different parameters of the testis and sperm.\u0000 \u0000 \u0000 \u0000 CoQ10 could, to some extent, prevent structural changes of the testis induced by the high dose of Sunset Yellow.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial 肌醇与二甲双胍对多囊卵巢综合征不孕妇女临床特征、内分泌和代谢特征的影响:随机对照试验
Q1 Veterinary Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390300
N.S.V. Le, Minh Tam Le, T. N. Cao
To compare the effectiveness of inositol and metformin on the clinical characteristics, and endocrine and metabolic profiles of infertile polycystic ovarian syndrome (PCOS) women from Vietnam. From June 2018 to August 2022, a randomized trial was undertaken at the Hue Center for Endocrinology and Reproduction on infertile women aged 18 to 40 years with polycystic ovarian syndrome. The clinical, endocrine, and metabolic features of these individuals were assessed before and after 3 months of treatment with 2 g of inositol or 1700 mg of metformin per day. Natural pregnancy rates, adverse effects, and tolerance of inositol were recorded. The study included 171 infertile PCOS women who were eligible to participate and took part in the baseline assessment, of whom 132 women participated in data analysis after 3 months. After metformin treatment, 42.1% of women with oligomenorrhea experienced regular menstruation. Metformin significantly lowered body mass index (BMI), waist circumference and testosterone levels, but had no effect on other clinical characteristics, endocrine profiles, or metabolic profiles. 29.2% Of women reported experiencing side effects. 21% Of them attained pregnancy, which resulted in 17.1% of live births. In the inositol group, the rate of regular cycle increased by 18.2% and the total testosterone concentration significantly decreased. In overweight/obese women with PCOS, inositol significantly decreased weight, BMI, waist and hip circumferences (P<0.05). 100% Of women tolerated inositol and continued treatment. 18.9% Of them became pregnant, leading to 17% of live births. Metformin and inositol can improve weight and waist circumference in overweight/obese infertile women with PCOS. Metformin is associated with a higher rate of regular menstruation, whereas inositol is associated with a lower rate of adverse effects. The spontaneous conception, clinical pregnancy, and live birth rates between two groups are comparable.
目的:比较肌醇和二甲双胍对越南多囊卵巢综合征(PCOS)不孕妇女的临床特征、内分泌和代谢特征的有效性。 2018年6月至2022年8月,顺化内分泌与生殖中心对18至40岁患有多囊卵巢综合征的不孕妇女进行了随机试验。在每天服用 2 克肌醇或 1700 毫克二甲双胍治疗 3 个月前后,对这些人的临床、内分泌和代谢特征进行了评估。同时还记录了自然怀孕率、不良反应以及对肌醇的耐受性。 该研究包括171名符合条件并参加了基线评估的多囊卵巢综合征不孕妇女,其中132名妇女参加了3个月后的数据分析。接受二甲双胍治疗后,42.1%的少经妇女月经正常。二甲双胍能明显降低体重指数(BMI)、腰围和睾酮水平,但对其他临床特征、内分泌特征或代谢特征没有影响。29.2%的妇女表示出现了副作用。其中 21% 的妇女怀孕,活产率为 17.1%。在肌醇组中,正常周期的比率增加了 18.2%,总睾酮浓度显著下降。在患有多囊卵巢综合症的超重/肥胖妇女中,肌醇能明显降低体重、体重指数、腰围和臀围(P<0.05)。100%的妇女能够耐受肌醇并继续接受治疗。其中 18.9% 的妇女怀孕,活产率为 17%。 二甲双胍和肌醇可以改善患有多囊卵巢综合症的超重/肥胖不孕妇女的体重和腰围。二甲双胍与较高的月经规律率有关,而肌醇与较低的不良反应率有关。两组患者的自然受孕率、临床妊娠率和活产率相当。
{"title":"Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial","authors":"N.S.V. Le, Minh Tam Le, T. N. Cao","doi":"10.4103/2305-0500.390300","DOIUrl":"https://doi.org/10.4103/2305-0500.390300","url":null,"abstract":"\u0000 \u0000 To compare the effectiveness of inositol and metformin on the clinical characteristics, and endocrine and metabolic profiles of infertile polycystic ovarian syndrome (PCOS) women from Vietnam.\u0000 \u0000 \u0000 \u0000 From June 2018 to August 2022, a randomized trial was undertaken at the Hue Center for Endocrinology and Reproduction on infertile women aged 18 to 40 years with polycystic ovarian syndrome. The clinical, endocrine, and metabolic features of these individuals were assessed before and after 3 months of treatment with 2 g of inositol or 1700 mg of metformin per day. Natural pregnancy rates, adverse effects, and tolerance of inositol were recorded.\u0000 \u0000 \u0000 \u0000 The study included 171 infertile PCOS women who were eligible to participate and took part in the baseline assessment, of whom 132 women participated in data analysis after 3 months. After metformin treatment, 42.1% of women with oligomenorrhea experienced regular menstruation. Metformin significantly lowered body mass index (BMI), waist circumference and testosterone levels, but had no effect on other clinical characteristics, endocrine profiles, or metabolic profiles. 29.2% Of women reported experiencing side effects. 21% Of them attained pregnancy, which resulted in 17.1% of live births. In the inositol group, the rate of regular cycle increased by 18.2% and the total testosterone concentration significantly decreased. In overweight/obese women with PCOS, inositol significantly decreased weight, BMI, waist and hip circumferences (P<0.05). 100% Of women tolerated inositol and continued treatment. 18.9% Of them became pregnant, leading to 17% of live births.\u0000 \u0000 \u0000 \u0000 Metformin and inositol can improve weight and waist circumference in overweight/obese infertile women with PCOS. Metformin is associated with a higher rate of regular menstruation, whereas inositol is associated with a lower rate of adverse effects. The spontaneous conception, clinical pregnancy, and live birth rates between two groups are comparable.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138993025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study 新鲜卵母细胞与玻璃化卵母细胞的胚胎、遗传和临床结果:回顾性队列研究
Q1 Veterinary Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390301
Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh
To compare embryonic development, ploidy status and clinical outcomes between fresh and frozen-thawed oocytes. This retrospective cohort study evaluated 83 fertilization cycles including both fresh and frozen oocytes from 79 patients at the HP Fertility Center of Hai Phong International Hospital of Obstetrics and Pediatrics in Vietnam. The patient underwent several ovarian stimulation cycles to accumulate a certain number of oocytes that would be vitrified. In the last oocyte retrieval, all patient's oocytes including both frozen and fresh would be fertilized. The outcomes included the rates of oocyte survival, cleavage embryo, blastocyst, ploidy status, pregnancy, biochemical pregnancy and clinical pregnancy. The oocyte survival rate after thawing was 96.5%. No statistically significant difference was found when comparing fresh and frozen oocytes regarding fertilization rate (78.1% vs. 75.5%, P=0.461), usable cleavage embryo rate (86.9% vs. 87.2%, P=0.916) but usable blastocyst rate was found higher statistically in the frozen oocyte group (44.4% vs. 54.0%, P=0.049). The percentages of euploid, aneuploid and mosaic embryos between the fresh group and the vitrified group had no significant differences (33.8% vs. 31.6%, P=0.682; 51.0% vs. 54.2%, P=0.569; 15.2% vs. 12.4%, P=0.787; respectively). The rates of pregnancy, biochemical pregnancy and clinical pregnancy had no statistical difference (68.8% vs. 64.8%, P=0.764; 12.5% vs. 3.6%, P=0.258; 37.5% vs. 46.4%, P=0.565). 17 Mature oocytes are the minimum to have at least one euploid embryo. Oocyte vitrification does not affect embryonic, genetic and clinical results. The number of mature oocytes should be considered for fertilization in some cases.
比较新鲜卵母细胞和冷冻解冻卵母细胞的胚胎发育、倍性状态和临床结果。 这项回顾性队列研究评估了越南海防国际产科和儿科医院 HP 生育中心 79 名患者的 83 个受精周期,包括新鲜和冷冻卵母细胞。患者接受了多个卵巢刺激周期,以积累一定数量的卵母细胞进行玻璃化处理。在最后一次取卵过程中,患者的所有卵母细胞(包括冷冻和新鲜卵母细胞)都将受精。结果包括卵母细胞存活率、卵裂胚胎率、囊胚率、倍性状态、妊娠率、生化妊娠率和临床妊娠率。 解冻后的卵母细胞存活率为 96.5%。新鲜卵母细胞和冷冻卵母细胞在受精率(78.1% 对 75.5%,P=0.461)和可用卵裂胚胎率(86.9% 对 87.2%,P=0.916)方面的差异无统计学意义,但冷冻卵母细胞组的可用囊胚率较高(44.4% 对 54.0%,P=0.049)。新鲜胚胎组和玻璃化胚胎组的非整倍体胚胎、非整倍体胚胎和马赛克胚胎的百分比没有显著差异(分别为 33.8% vs. 31.6%,P=0.682;51.0% vs. 54.2%,P=0.569;15.2% vs. 12.4%,P=0.787)。妊娠率、生化妊娠率和临床妊娠率无统计学差异(68.8% vs. 64.8%,P=0.764;12.5% vs. 3.6%,P=0.258;37.5% vs. 46.4%,P=0.565)。17 成熟卵母细胞至少要有一个单倍体胚胎。 卵母细胞玻璃化不会影响胚胎、遗传和临床结果。在某些情况下,受精时应考虑成熟卵母细胞的数量。
{"title":"Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study","authors":"Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh","doi":"10.4103/2305-0500.390301","DOIUrl":"https://doi.org/10.4103/2305-0500.390301","url":null,"abstract":"\u0000 \u0000 To compare embryonic development, ploidy status and clinical outcomes between fresh and frozen-thawed oocytes.\u0000 \u0000 \u0000 \u0000 This retrospective cohort study evaluated 83 fertilization cycles including both fresh and frozen oocytes from 79 patients at the HP Fertility Center of Hai Phong International Hospital of Obstetrics and Pediatrics in Vietnam. The patient underwent several ovarian stimulation cycles to accumulate a certain number of oocytes that would be vitrified. In the last oocyte retrieval, all patient's oocytes including both frozen and fresh would be fertilized. The outcomes included the rates of oocyte survival, cleavage embryo, blastocyst, ploidy status, pregnancy, biochemical pregnancy and clinical pregnancy.\u0000 \u0000 \u0000 \u0000 The oocyte survival rate after thawing was 96.5%. No statistically significant difference was found when comparing fresh and frozen oocytes regarding fertilization rate (78.1% vs. 75.5%, P=0.461), usable cleavage embryo rate (86.9% vs. 87.2%, P=0.916) but usable blastocyst rate was found higher statistically in the frozen oocyte group (44.4% vs. 54.0%, P=0.049). The percentages of euploid, aneuploid and mosaic embryos between the fresh group and the vitrified group had no significant differences (33.8% vs. 31.6%, P=0.682; 51.0% vs. 54.2%, P=0.569; 15.2% vs. 12.4%, P=0.787; respectively). The rates of pregnancy, biochemical pregnancy and clinical pregnancy had no statistical difference (68.8% vs. 64.8%, P=0.764; 12.5% vs. 3.6%, P=0.258; 37.5% vs. 46.4%, P=0.565). 17 Mature oocytes are the minimum to have at least one euploid embryo.\u0000 \u0000 \u0000 \u0000 Oocyte vitrification does not affect embryonic, genetic and clinical results. The number of mature oocytes should be considered for fertilization in some cases.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro 低温保存的绵羊精原干细胞在体外保持干性和集落形成能力
Q1 Veterinary Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390302
R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra
To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells (SSCs) in vitro. Sheep testicular cells were isolated and putative SSCs were enriched by the laminin-based differential plating method. Putative SSCs were co-cultured with the Sertoli cell feeder prepared by the Datura Stramonium Agglutinin (DSA-lectin)-based method. The cultured putative SSCs were cryopreserved in Dulbecco's Modified Eagle Medium-10% fetal bovine serum mixture (DMEM-10% FBS) media containing 10% dimethyl sulfoxide (DMSO) alone or 10% DMSO plus 200 mM trehalose. Cryopreserved putative SSCs were evaluated for their proliferation potential using in vitro culture and stemness by immunocytochemistry. Finally, the transfection ability of cryopreserved putative SSCs was analyzed. We isolated 91% viable testicular cells from sheep testes. The majority of the laminin enriched cells expressed the SSC related marker, ITGA6. Co-culture of sheep putative SSCs with Sertoli cell feeder resulted in the generation of stable colonies, and the expression of SSC marker was maintained after several passages. A significantly higher number of viable putative SSCs was recovered from SSCs cryopreserved in media containing 10% DMSO and 200 mM trehalose compared to 10% DMSO alone (P<0.01). Cryopreserved putative SSCs formed colonies and showed SSC marker expression similar to the non-cryopreserved putative SSCs. The appearance of green fluorescent colonies over the Sertoli cell feeder indicated that cryopreserved sheep SSCs were successfully transfected. Cryopreserved putative SSCs can retain their stemness, colony forming ability, and transfection efficiency in vitro. Our research may help in the effective preservation of germplasm and the generation of transgenic ovine species.
评估低温保存对体外绵羊精原干细胞(SSCs)的干性和增殖潜力的影响。 分离绵羊睾丸细胞,并通过基于层粘连素的差分平板法富集推定的精原干细胞。将推定的SSCs与基于曼陀罗凝集素(DSA-lectin)方法制备的Sertoli细胞供养体共同培养。培养出的推定 SSCs 被冷冻保存在含 10%二甲基亚砜(DMSO)或 10%二甲基亚砜加 200 mM 曲哈糖的杜氏改良鹰培养基-10%胎牛血清混合液(DMEM-10% FBS)培养基中。利用体外培养和免疫细胞化学方法对冷冻保存的推定 SSCs 的增殖潜力和干性进行评估。最后,分析了冷冻保存的推定 SSCs 的转染能力。 我们从绵羊睾丸中分离出了91%存活的睾丸细胞。大部分富含层粘连蛋白的细胞表达了与SSC相关的标志物ITGA6。绵羊推定SSC细胞与Sertoli细胞饲养剂共培养可产生稳定的集落,SSC标记的表达在多次传代后仍能保持。在含10%二甲基亚砜和200毫摩尔曲哈洛糖的培养基中冷冻保存的SSC与仅含10%二甲基亚砜的培养基相比,能存活的推定SSC数量明显更高(P<0.01)。冷冻保存的假定 SSCs 形成了菌落,并显示出与非冷冻保存的假定 SSCs 相似的 SSC 标记表达。Sertoli细胞饲养器上出现绿色荧光菌落表明冷冻保存的绵羊SSCs转染成功。 低温保存的推定造血干细胞可保持其干性、集落形成能力和体外转染效率。我们的研究可能有助于种质的有效保存和转基因绵羊的产生。
{"title":"Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro","authors":"R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra","doi":"10.4103/2305-0500.390302","DOIUrl":"https://doi.org/10.4103/2305-0500.390302","url":null,"abstract":"\u0000 \u0000 To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells (SSCs) in vitro.\u0000 \u0000 \u0000 \u0000 Sheep testicular cells were isolated and putative SSCs were enriched by the laminin-based differential plating method. Putative SSCs were co-cultured with the Sertoli cell feeder prepared by the Datura Stramonium Agglutinin (DSA-lectin)-based method. The cultured putative SSCs were cryopreserved in Dulbecco's Modified Eagle Medium-10% fetal bovine serum mixture (DMEM-10% FBS) media containing 10% dimethyl sulfoxide (DMSO) alone or 10% DMSO plus 200 mM trehalose. Cryopreserved putative SSCs were evaluated for their proliferation potential using in vitro culture and stemness by immunocytochemistry. Finally, the transfection ability of cryopreserved putative SSCs was analyzed.\u0000 \u0000 \u0000 \u0000 We isolated 91% viable testicular cells from sheep testes. The majority of the laminin enriched cells expressed the SSC related marker, ITGA6. Co-culture of sheep putative SSCs with Sertoli cell feeder resulted in the generation of stable colonies, and the expression of SSC marker was maintained after several passages. A significantly higher number of viable putative SSCs was recovered from SSCs cryopreserved in media containing 10% DMSO and 200 mM trehalose compared to 10% DMSO alone (P<0.01). Cryopreserved putative SSCs formed colonies and showed SSC marker expression similar to the non-cryopreserved putative SSCs. The appearance of green fluorescent colonies over the Sertoli cell feeder indicated that cryopreserved sheep SSCs were successfully transfected.\u0000 \u0000 \u0000 \u0000 Cryopreserved putative SSCs can retain their stemness, colony forming ability, and transfection efficiency in vitro. Our research may help in the effective preservation of germplasm and the generation of transgenic ovine species.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands 季节应激对安达曼和尼科巴群岛濒危泰瑞莎山羊精液质量、精液生化和氧化应激属性的影响
Q1 Veterinary Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390304
P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar
To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons. A total of 10 Teressa bucks (3-4 years) were selected from breeding farm, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India. Semen samples (n=25 per season) were collected through artificial vagina method and preserved at refrigerated temperature (5°C) for 48 h using Tris citrate glucose based extender. We detected semen quality parameters [volume, mass activity, pH, sperm concentration, total motility, viability, total sperm abnormality, and plasma membrane, acrosomal and nuclear integrities], biochemical profiles [aspartate amino transferase (AST), alanine amino transferase (ALT) and total cholesterol], and oxidative stress markers [total antioxidant capacity (TAC) and malondialdehyde (MDA)] during monsoon and dry seasons. Semen quality parameters significantly differed between seasons (P<0.05) and among storage periods (P<0.05). Volume, pH, mass activity, motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly higher (P<0.05). Sperm concentration, sperm abnormalities, MDA, AST, ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season (P<0.05). Motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly decreased (P<0.05) while sperm abnormality, AST, ALT, total cholesterol and MDA were significantly increased as liquid semen storage period advanced (P<0.05). Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h. Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination.
测量季风和干旱季节对新鲜精液和液体储存精液的精液质量、精液生化和氧化应激属性的季节性影响。 从印度安达曼和尼科巴群岛布莱尔港的ICAR-中央岛屿农业研究所繁殖场选取了10头Teressa公鹿(3-4岁)。通过人工阴道法采集精液样本(每季 25 份),并使用柠檬酸特里斯葡萄糖基延长剂在冷藏温度(5°C)下保存 48 小时。我们检测了季风季节和旱季的精液质量参数[体积、质量活性、pH值、精子浓度、总活力、存活率、精子畸形总数以及质膜、顶体和核完整性]、生化指标[天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)和总胆固醇]以及氧化应激指标[总抗氧化能力(TAC)和丙二醛(MDA)]。 精液质量参数在不同季节(P<0.05)和不同储存期(P<0.05)之间存在明显差异。体积、pH值、质量活性、运动性、存活率、顶体、质膜和核整合度以及TAC都明显较高(P<0.05)。季风季节新鲜精液中的精子浓度、精子畸形率、MDA、AST、ALT 和总胆固醇明显低于旱季(P<0.05)。随着精液贮存期的延长,精子活力、顶体、质膜和核完整性以及TAC明显降低(P<0.05),而精子畸形率、谷草转氨酶、谷丙转氨酶、总胆固醇和MDA则明显升高(P<0.05)。 季风季节对精液质量的影响更大,液态精液储存48小时后仍可使用。
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Asian Pacific Journal of Reproduction
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