In silico and in vitro assessment of yellowfin tuna skin (Thunnus albacares) hydrolysate antioxidation effect

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-02-18 DOI:10.1016/j.fhfh.2023.100126
Dian Wahyu Wardani , Andriati Ningrum , Manikharda , Nurul Vanidia , Heli Siti Helimatul Munawaroh , Eko Susanto , Pau-Loke Show
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引用次数: 4

Abstract

The protein hydrolysate that contains bioactive peptides of yellowfin tuna (Thunnus albacares) skin collagen with antioxidant activity has been successfully studied by using in silico and in vitro assays. We found that using the in silico assessment, the antioxidant peptides can be found from the precursor (type I α1 and α2 collagen of yellowfin tuna). Applying papain as a protease will also provide the greatest degree of hydrolysis for antioxidative peptides. The highest peptide rank peptides sequence such as Pro-Trp-Gly (PWG), Pro-His-Gly (PHG), His-Leu (HL), Ile-Arg (IR), Ala-His (AH), Glu-Leu (EL) that predicted using papain in silico. Molecular docking analysis showed all peptides derived from yellowfin tuna have hindered the substrate to access the active site of myeloperoxidase (MPO). Interestingly, the substitution of the amino acid from His (PHG, 7.1 kcal/mol) to Trp (PWG, 8.0 kcal/mol) has increased the affinity of the peptide towards MPO. They have antioxidative activities used in silico approach to MPO enzyme. We also confirm the in vitro assays for the protein hydrolysate after proteolysis using papain. The concentration and hydrolysis time will give influence the degree of hydrolysis, and antioxidant activities (P < 0.05). In conclusion, hydrolysate protein of type I α1 and α2 collagen from yellowfin tuna produced by papain hydrolysis has the potential to be used in food, active packaging material until health applications..

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黄鳍金枪鱼皮肤(Thunnus albacares)水解物抗氧化作用的体外和室内评价
采用硅法和体外实验成功地研究了黄鳍金枪鱼皮肤胶原蛋白中含有抗氧化活性肽的蛋白水解产物。我们发现,通过计算机评价,可以从黄鳍金枪鱼的前体(α1和α2型胶原蛋白)中找到抗氧化肽。将木瓜蛋白酶作为蛋白酶也将为抗氧化肽提供最大程度的水解。利用木瓜蛋白酶预测的最高肽阶肽序列为Pro-Trp-Gly (PWG)、Pro-His-Gly (PHG)、His-Leu (HL)、Ile-Arg (IR)、Ala-His (AH)、Glu-Leu (EL)。分子对接分析表明,所有黄鳍金枪鱼衍生的肽都阻碍了底物进入髓过氧化物酶(MPO)活性位点。有趣的是,从His (PHG, 7.1 kcal/mol)到Trp (PWG, 8.0 kcal/mol)的氨基酸替换增加了肽对MPO的亲和力。它们具有抗氧化活性,用硅法对MPO酶进行了研究。我们还证实了木瓜蛋白酶水解后蛋白水解产物的体外测定。浓度和水解时间会给水解程度、抗氧化活性(P <0.05)。综上所述,木瓜蛋白酶水解黄鳍金枪鱼制备的ⅰ型α1和α2型胶原蛋白具有应用于食品、活性包装材料直至保健应用的潜力。
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CiteScore
4.50
自引率
0.00%
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审稿时长
61 days
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