Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro:a study emphasizing signaling molecule expression and transcriptional changes

IF 1.1 4区 生物学 Q3 BIOLOGY Turkish Journal of Biology Pub Date : 2017-10-01 DOI:10.3906/BIY-1701-23
Qiong Wu, Chang-xin Jin, Hui Chen, Xueyang Li, Yue Li
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引用次数: 1

Abstract

Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were significantly enhanced in both protein (Smad, Slug, Snail1/2, Twist1, N-cadherin, vimentin) and mRNA (SMAD, SLUG, SNAIL1/2, TWIST1, N-cadherin, and vimentin) levels. The expression levels of oncogenes (RAS and HER-2) and an antioncogene gene (P53) in ADSCs were upregulated and downregulated, respectively. Interestingly, variation tendencies of the molecules were more conspicuous in inflammatory circumstances than without. In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.
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脂肪源性间充质干细胞与MCF-7细胞的相互作用:一项强调信号分子表达和转录变化的研究
脂肪源性干细胞(ADSCs)促进乳腺癌细胞的转移,并能在肿瘤微环境中分化为癌相关细胞。然而,人们对其确切机制知之甚少。本研究表明,ADSCs与乳腺癌MCF-7细胞的相互作用改变了MCF-7细胞中转移相关功能蛋白的水平,也改变了ADSCs中癌基因的水平。从患者脂肪组织中分离ADSCs。通过将ADSCs(或MCF-7细胞)与MCF-7细胞(或ADSCs)衍生的外泌体共培养,ADSCs与MCF-7细胞相互作用。外泌体用DiI标记。在共培养中,MCF-7细胞中迁移相关调节因子的蛋白(Smad、Slug、Snail1/2、Twist1、N-cadherin、vimentin)和mRNA (Smad、Slug、Snail1/2、Twist1、N-cadherin和vimentin)水平均显著增强。癌基因(RAS和HER-2)和抗癌基因(P53)在ADSCs中的表达水平分别上调和下调。有趣的是,分子的变异趋势在炎症环境中比在没有炎症的情况下更为明显。综上所述,MCF-7细胞与ADSCs的相互作用增加了MCF-7细胞中一系列转移相关功能蛋白的水平,并增强了ADSCs中癌基因的表达。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
20
审稿时长
6-12 weeks
期刊介绍: The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.
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