Application of green-extraction technique to evaluate of antioxidative capacity of wild population of fireweed (Epilobium angustifolium)

Q3 Medicine Herba Polonica Pub Date : 2019-12-01 DOI:10.2478/hepo-2019-0022

A. Nowak, A. Klimowicz, Wiktoria Duchnik, Ł. Kucharski, Katarzyna Florkowska, Anna Muzykiewicz, D. Wira, Joanna Zielonkabrzezicka, Anita Siedłowska, Kinga Nadarzewska

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引用次数: 19

Abstract

Summary Introduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields. Objective: The aim of the study was to evaluate the antioxidant activity of extracts from fresh fireweed, harvested at three ripening stages. Methods: Analysis of antioxidative activity was carried out by DPPH, ABTS and FRAP methods. Total polyphenol and total flavonoid content were also determined. Plant material was extracted using ultra-sound-assisted green extraction technique with methanol, ethanol and isopropanol at different concentrations and water. Results: The highest antioxidant activity evaluated by DPPH, ABTS and FRAP was found for the extracts prepared in 70% ethanol. The highest content of total polyphenols were observed in extracts in 70% ethanol, whereas the highest content of flavonoids extracts in undiluted methanol. Conclusion: Epilobium angustifolium harvested at fruit ripening stage seems to be a valuable source of antioxidants.

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绿色提取技术在野生火杂草抗氧化能力评价中的应用

简介：火苗（Epilobium angustifolium（L.）Holub）是一种常见的杂草，生长在草地、路边和农业荒地上，形成了广阔、快速蔓延的田地。目的：本研究的目的是评价在三个成熟阶段收获的新鲜火麻提取物的抗氧化活性。方法：采用DPPH法、ABTS法和FRAP法进行抗氧化活性分析。还测定了总多酚和总黄酮的含量。采用超声波辅助绿色提取技术，以不同浓度的甲醇、乙醇、异丙醇和水提取植物材料。结果：DPPH、ABTS和FRAP评价70%乙醇提取物的抗氧化活性最高。70%乙醇提取物中总多酚含量最高，而未稀释的甲醇提取物中黄酮类化合物含量最高。结论：在果实成熟期收获的狭叶是一种有价值的抗氧化剂来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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