Statistical optimization for polyhydroxybutyrate production by locally isolated Bacillus safensis using sugarcane molasses under nutritional stressed conditions
Sanaa K. Gomaa, H. El-Refai, R. Allam, M. Shafei, H. Ahmed, Rania A. Zaki
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引用次数: 0
Abstract
Background and objective Biodegradable polymers, such as polyhydroxyalkanoate (PHAs), have recently been recognized as polyesters owing to their properties as biodegradable thermoplastics. Materials and methods The main objective of this study was to isolate an efficient polyhydroxybutyrate (PHB) producer from soil collected from different rhizospheric areas in Egypt. The potent strain was identified using 16 s rRNA sequence analysis. Plackett–Burman and central composite sequential designs were used to investigate cultural variables influencing PHB production (central composite sequential design). Results and conclusion Of a total of nine different isolates, three were found to be PHB positive based on the color using Nile Red stain. The potent strain was identified as Bacillus safensis (NR_113945.1). Using one factor at a time experiments, sugar molasses and ammonium sulfate, respectively, were the best carbon and nitrogen sources, whereas the best inoculum was 10 ml/100 ml of fermentation medium. Cultural variables influencing PHB production were studied using Plackett–Burman and central composite sequential designs. Accordingly, the most influential factors on PHB production were sugarcane concentration, inoculum size, and KH2PO4. Under the optimized conditions, a PHB content (93% cell dry weight) of 1.17 folds was attained. Furthermore, the Fourier transform infrared spectroscopy and 1H-NMR results confirmed the produced polymer as PHB. These results give insight into the use of locally isolated bacteria (B. safensis (NR_113945.1) utilizing inexpensive substrate such as sugarcane molasses for PHB production.
背景和目的可生物降解聚合物,如聚羟基烷酸酯(PHA),由于其作为可生物降解热塑性塑料的特性,最近被认为是聚酯。材料和方法本研究的主要目的是从埃及不同根际地区收集的土壤中分离出一种高效的聚羟基丁酸(PHB)生产商。使用16 s rRNA序列分析。Plackett–Burman和中心复合序列设计用于研究影响PHB生产的文化变量(中心复合序列)。结果和结论在总共9个不同的分离株中,根据尼罗红染色的颜色,发现3个PHB阳性。经鉴定,该菌株为安全芽孢杆菌(NR_113945.1)。采用一因素一次试验,糖蜜和硫酸铵分别是最佳的碳源和氮源,而最佳接种量为10 ml/100 ml发酵培养基。使用Plackett–Burman和中心复合序列设计研究了影响PHB生产的文化变量。因此,对PHB生产影响最大的因素是甘蔗浓度、接种量和KH2PO4。在优化的条件下,PHB含量(93%的细胞干重)达到1.17倍。此外,傅立叶变换红外光谱和1H-NMR结果证实所产生的聚合物为PHB。这些结果深入了解了利用廉价基质如甘蔗糖蜜生产PHB的本地分离细菌(B.safensis(NR_113945.1))的用途。