Functional characterization of the Arabidopsis actin 2 promoter in cotton (Gossypium hirsutum L.) flowers and bolls

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2018-09-01 DOI:10.1016/j.aggene.2018.07.002

Estela Reis de Andrade , Francisco José Lima Aragão

引用次数: 0

Abstract

Efforts have been made to engineer cotton to achieve resistance to the boll weevil, which is one of the most important pests impacting cotton production in the Americas. The insects feed on flowers and immature cotton bolls. Strategies for expressing insecticide proteins must be associated with a strong expression in these tissues. The expression of the gus gene driven by the ACT2 promoter from Arabidopsis thaliana was compared to the 35SCaMV promoter in leaves, roots, petals and fiber of transgenic cotton plants. Fluorometric and histochemical analyses have shown that the ACT2 promoter produced a strong expression in floral and boll tissues, including pollen, petals, ovules and fiber. It revealed the potential of using the ACT2 promoter to express genes involved with boll weevil resistance.

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拟南芥肌动蛋白2启动子在棉花花和铃中的功能研究

棉铃虫是影响美洲棉花生产的最重要害虫之一，人们已经努力对棉花进行基因改造，使其能够抵抗棉铃虫。这种昆虫以花和未成熟的棉铃为食。表达杀虫剂蛋白的策略必须与这些组织中的强表达相关。将拟南芥中ACT2启动子驱动的gus基因与35SCaMV启动子在转基因棉花叶片、根、花瓣和纤维中的表达进行比较。荧光和组织化学分析表明，ACT2启动子在花粉、花瓣、胚珠和纤维等花和棉铃组织中均有强烈表达。这揭示了利用ACT2启动子表达棉铃虫抗性相关基因的潜力。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.

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