Abad Hurtado, J. Flores-Santos, C. N. Flores-Fernández, S. Saavedra, João H. P. M. Santos, A. Pessoa-Júnior, M. Lienqueo, M. Bayro, A. Zavaleta
{"title":"A Novel L-Asparaginase from Enterobacter sp. Strain M55 from Maras Salterns in Peru","authors":"Abad Hurtado, J. Flores-Santos, C. N. Flores-Fernández, S. Saavedra, João H. P. M. Santos, A. Pessoa-Júnior, M. Lienqueo, M. Bayro, A. Zavaleta","doi":"10.15255/cabeq.2022.2064","DOIUrl":null,"url":null,"abstract":"L-Asparaginase (ASNase) is used in medicine for neoplasms treatment and in food industry for mitigation of acrylamide in high-temperature processed food. In medicine, commercial ASNases have exhibited side effects and l -glutaminase (GLNase) activity affecting the clinical treatment. The aim of this work was to study a novel ASNase from Enterobacter sp. M55 isolated from Maras Salters in Peru, which was purified and biochemically characterised. This ASNase exhibited a K m of 5.71 mM and a V max of 0.16 µmol mL –1 min –1 , as well as an optimum temperature and pH of 37 °C and 6, respectively. Moreover, a good activity (80 %) was observed at physiological pH. Likewise, the enzyme increased its activity by around 50% in presence of urea, glutathione, and glucose. Whilst in presence of serum compounds, it kept more that 60 % of activity. In addition, this ASNase showed low GLNase activity.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biochemical Engineering Quarterly","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.15255/cabeq.2022.2064","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
L-Asparaginase (ASNase) is used in medicine for neoplasms treatment and in food industry for mitigation of acrylamide in high-temperature processed food. In medicine, commercial ASNases have exhibited side effects and l -glutaminase (GLNase) activity affecting the clinical treatment. The aim of this work was to study a novel ASNase from Enterobacter sp. M55 isolated from Maras Salters in Peru, which was purified and biochemically characterised. This ASNase exhibited a K m of 5.71 mM and a V max of 0.16 µmol mL –1 min –1 , as well as an optimum temperature and pH of 37 °C and 6, respectively. Moreover, a good activity (80 %) was observed at physiological pH. Likewise, the enzyme increased its activity by around 50% in presence of urea, glutathione, and glucose. Whilst in presence of serum compounds, it kept more that 60 % of activity. In addition, this ASNase showed low GLNase activity.
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