Inhibitory effect of essential oils from Pulicaria mauritanica and Micromeria debilis on growth of Alternaria spp., the causal agent of tomato early blight

Environmental and Experimental Biology Pub Date : 2019-12-31 DOI:10.22364/eeb.17.19

Meryem Yakoubi, N. Hamini-Kadar, M. Gherib, Abdelilah Amrouche, W. Yezli, S. Benichou, M. Kihal

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引用次数: 2

Abstract

The aim of the present study was the evaluation of antifungal activity of essential oil (EO) extracted from two medicinal and aromatic plants (Pulicaria mauritanica and Micromeria debilis) as biological treatment for Alternaria spp., a pathogenic mould that causes early blight on tomato plants. EO was extracted by hydrodistillation and antifungal activity was analyzed in vitro using direct contact on agar against ten isolates of Alternaria spp. Extraction yield was 0.30 and 0.045% for P. mauritanica and M. debilis, respectively. Both EOs had inhibitory effect against mycelial growth of all studied isolates. However, the antifungal effect of EO isolated from P. mauritanica was stronger than for that from M. debilis, with 100% inhibition against Alternaria tenuissema (isolates A18 and A57), Alternaria alternata (isolate AT4), Alternaria solani (A37) and Alternaria tomatophila (AT01), when used at 1.1 μL mL–1 concentration. EO from M. debilis had 100% inhibition against isolates A42 and A57 of A. solani and A. tenuissema when used at 1.1 μL mL–1 concentration. The antifungal activity of the EOs might due to the synergic effects of their chemical compounds, mainly monoterpenes and sesquiterpenes.

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茅利塔尼根挥发油和小细穗挥发油对番茄早疫病病原菌赤霉生长的抑制作用

本研究的目的是评估从两种药用植物和芳香植物（毛普利卡菌和debilis微eria）中提取的精油（EO）作为Alternaria spp.的生物治疗药物的抗真菌活性。Alternaria是一种引起番茄早期枯萎病的病原霉菌。采用水蒸馏法提取EO，并在琼脂上直接接触对10株链格孢进行体外抗真菌活性分析。毛孢霉和debilis霉的提取率分别为0.30%和0.045%。两种EOs对所有研究菌株的菌丝生长都有抑制作用。然而，当以1.1μL mL–1的浓度使用时，毛孢霉中分离的EO的抗真菌作用强于debilis中的EO，对细链格孢（分离株A18和A57）、Alternaria alternata（分离株AT4）、Alterania solani（A37）和Alternaria tomatophila（AT01）具有100%的抑制作用。当以1.1μL mL–1的浓度使用时，来自M.debilis的EO对A.solani和A.tenuisema的分离株A42和A57具有100%的抑制作用。EOs的抗真菌活性可能是由于其化合物（主要是单萜和倍半萜）的协同作用。

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