Expressions of hypoxia inducible factor-1α and autophagy related protein Beclin-1 in colon cancer and their clinical significances

Q4 Medicine 肿瘤研究与临床 Pub Date : 2019-11-28 DOI:10.3760/CMA.J.ISSN.1006-9801.2019.11.007

Zhongguo Li, Yaoqiang Wu, Hongwei Xue

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Abstract

Objective To investigate the expressions of hypoxia inducible factor-1α (HIF-1α) and autophagy related protein Beclin-1 in colon cancer and their clinical significances. Methods From January 2017 to December 2018, 120 patients with colon cancer who were admitted to Dandong First Hospital were selected. The expressions of HIF-1α and Beclin-1 in colon cancer and corresponding adjacent tissues were detected by immunohistochemical SABC method. The relationship of the expressions of HIF-1α and Beclin-1 with the clinicopathological features of colon cancer patients was also discussed. The expressions of HIF-1α and Beclin-1 in 20 pairs of fresh colon cancer and paracancerous tissues were detected by Western blot. Results The results of immunohistochemistry showed that the positive expression rates of HIF-1α and Beclin-1 proteins in colon cancer tissues were significantly higher than those in paracancerous tissues [75.0% (90/120) vs. 21.7% (26/120), 60.8% (73/120) vs. 12.5% (15/120)], and the differences were statistically significant (χ 2 values were 68.343 and 60.359, both P 0.05). There was a positive correlation between the expressions of HIF-1α and Beclin-1 proteins in colon cancer (r = 0.483, P < 0.01). Conclusion The expressions of HIF-1α and Beclin-1 proteins in colon cancer tissues are significantly increased, and the interaction between them is involved in the occurrence and development of colon cancer. Key words: Colon neoplasms; Hypoxia-inducible factor 1, alpha subunit; Gene, Beclin-1; Autophagy

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低氧诱导因子-1α和自噬相关蛋白Beclin-1在结肠癌中的表达及临床意义

目的探讨缺氧诱导因子-1α（HIF-1α）和自噬相关蛋白Beclin-1在结肠癌组织中的表达及其临床意义。方法选择2017年1月至2018年12月收治在丹东市第一医院的120例癌症结肠癌患者。应用免疫组织化学SABC法检测结肠癌及其癌旁组织中HIF-1α和Beclin-1的表达。探讨了HIF-1α和Beclin-1的表达与结肠癌临床病理特征的关系。用蛋白质印迹法检测了20对新鲜结肠癌组织和癌旁组织中HIF-1α和Beclin-1的表达。结果免疫组化结果显示，结肠癌组织中HIF-1α和Beclin-1蛋白的阳性表达率显著高于癌旁组织[75.0%（90/120）vs.21.7%（26/120），60.8%（73/120）vs12.5%（15/120）]，2值分别为68.343和60.359，均P＜0.05。结肠癌组织中HIF-1α和Beclin-1蛋白表达呈正相关（r＝0.483，P＜0.01），它们之间的相互作用参与了癌症的发生和发展。关键词：结肠肿瘤；缺氧诱导因子1，α亚基；基因，Beclin-1；自噬

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来源期刊

肿瘤研究与临床 Medicine-Oncology

CiteScore

0.10

自引率

0.00%

发文量

7737

期刊介绍： "Cancer Research and Clinic" is a series of magazines of the Chinese Medical Association under the supervision of the National Health Commission and sponsored by the Chinese Medical Association. It mainly reflects scientific research results and academic trends in the field of malignant tumors. The main columns include monographs, guidelines and consensus, standards and norms, treatises, short treatises, survey reports, reviews, clinical pathology (case) discussions, case reports, etc. The readers are middle- and senior-level medical staff engaged in basic research and clinical work on malignant tumors.