Hydroxyapatite Modified Silica Aerogel Nanoparticles: In Vitro Cell Migration Analysis

Q3 Biochemistry, Genetics and Molecular Biology Biointerface Research in Applied Chemistry Pub Date : 2022-10-07 DOI:10.33263/briac134.373

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Abstract

The ability of silica aerogel nanoparticles (SA-Np) to improve the stability of hydroxyapatite (HA) was investigated. Using the sol-gel method, the HA was incorporated into SA-Np at a weight ratio of 0.5 of HA to SiO2 (HA-SA-Np). The efficacy of HA-SA-Np, SA-Np, and HA on the in vitro migration of normal human dermal fibroblast cells (HSF1184) was compared. The cell migration was measured at 0, 6, and 24 hours after scratching using ImageJ and an inverted optical microscope. To ascertain the resorbability of HA-SA-Np, the phosphate and silicic acid concentrations in media treated for 2, 5, and 7 days were examined. The high dissolution of HA could be reduced by incorporating the HA into the silica nanosphere. The HA-SA-Np significantly stimulated cell migration and increased closure with increasing treatment time. It was caused by the release of silicic acid, which aided in healing cells. It also demonstrates the ability of HA-SA-Np to be resorbed and eventually increase the adhesion and migration of normal human fibroblast cells. As a result, the potential application of HA-SA-Np as an alternative biomaterial was confirmed.

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羟基磷灰石改性二氧化硅气凝胶纳米粒子的体外细胞迁移分析

研究了二氧化硅气凝胶纳米粒子（SA Np）改善羟基磷灰石（HA）稳定性的能力。使用溶胶-凝胶法，将HA以HA与SiO2（HA-SA-Np）的0.5的重量比掺入SA-Np中。比较了HA、SA和HA对正常人真皮成纤维细胞（HSF1184）体外迁移的影响。使用ImageJ和倒置光学显微镜在刮擦后0、6和24小时测量细胞迁移。为了确定HA-SA-Np的可吸收性，检测处理2、5和7天的培养基中的磷酸盐和硅酸浓度。通过将HA掺入二氧化硅纳米球中，可以降低HA的高溶解性。HA SA Np显著刺激细胞迁移，并随着处理时间的增加而增加闭合。它是由硅酸的释放引起的，硅酸有助于细胞的愈合。它还证明了HA-SA-Np被再吸收并最终增加正常人成纤维细胞的粘附和迁移的能力。因此，HA-SA-Np作为一种替代生物材料的潜在应用得到了证实。

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来源期刊

Biointerface Research in Applied Chemistry CHEMISTRY, APPLIED-

CiteScore

4.80

自引率

0.00%

发文量

256

期刊介绍： Biointerface Research in Applied Chemistry is an international and interdisciplinary research journal that focuses on all aspects of nanoscience, bioscience and applied chemistry. Submissions are solicited in all topical areas, ranging from basic aspects of the science materials to practical applications of such materials. With 6 issues per year, the first one published on the 15th of February of 2011, Biointerface Research in Applied Chemistry is an open-access journal, making all research results freely available online. The aim is to publish original papers, short communications as well as review papers highlighting interdisciplinary research, the potential applications of the molecules and materials in the bio-field. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible.