Complete genome sequence of Roseivivax marinus strain TCYB24 with quorum sensing system reveal the adaptive mechanism against deep-sea hydrothermal environment

Abstract

Roseivivax marinus strain TCYB24 is a rod-shaped bacterium of Rhodobacteraceae isolated from the gill of deep-sea mussel Bathymodiolus marisindicus which collected from the Tiancheng hydrothermal vent under depth of 2700 m on the southwest Indian ridge. In our previous study, the strain TCYB24 was proved to produce quorum sensing signal of N-Acyl-homoserine lactones (AHLs) and form biofilm. In order to determine its adaptive mechanism against the extreme environment of deep-sea hydrothermal vents, the whole genome was sequenced by high-throughput Illumina tag sequencing. The results show the whole genome consists of one circular chromosome and eight circular plasmids, with a total length of 4.60 Mb (G + C content of 67.4%), 4338 open reading frames, 46 tRNAs and 6 rRNA operons. According to the genome-wide functional annotation, numbers of heavy metal resistance, high pressure and cold adapting related genes were found. In addition, genes about exopolysaccharide (EPS) biosynthesis and secretion and biofilm formation, which facilitate bacteria to resist extreme environments, were identified. Intriguingly, a pair of RaiI/R-type quorum sensing system was discovered firstly in the bacterium isolated from hydrothermal environment. The results may help to understand genetic underpinning of extreme environmental adaptation mechanism of bacteria in deep-sea hydrothermal area.