{"title":"Circular mRNA: A novel therapeutic agent","authors":"Xiaoxue Wang , Jian Dong , Yuan Lu","doi":"10.1016/j.biotno.2023.09.001","DOIUrl":null,"url":null,"abstract":"<div><p>Circular mRNA (circmRNA) is a covalent closed loop formed by reverse splicing of the 3′ end to the 5′ end of mRNA. Compared to traditional linear mRNAs, circmRNAs can mediate efficient, stable, and durable protein expression and are considered an alternative to linear mRNAs in terms of therapeutic reagents. With the continuous development of circmRNA research, circmRNA has also made significant progress in vaccines and cellular therapies. In this review, we present research advances in the <em>in vitro</em> synthesis of circmRNAs, focusing on the biological ligation methods of circmRNAs and current applications, with a summary of challenges regarding circmRNA design, synthesis, and applications. Based on the enhanced stability of circmRNAs, further research on circmRNAs could help expand their applications in biotherapeutics and strengthen their role in basic medical applications.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 58-63"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Notes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2665906923000053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Circular mRNA (circmRNA) is a covalent closed loop formed by reverse splicing of the 3′ end to the 5′ end of mRNA. Compared to traditional linear mRNAs, circmRNAs can mediate efficient, stable, and durable protein expression and are considered an alternative to linear mRNAs in terms of therapeutic reagents. With the continuous development of circmRNA research, circmRNA has also made significant progress in vaccines and cellular therapies. In this review, we present research advances in the in vitro synthesis of circmRNAs, focusing on the biological ligation methods of circmRNAs and current applications, with a summary of challenges regarding circmRNA design, synthesis, and applications. Based on the enhanced stability of circmRNAs, further research on circmRNAs could help expand their applications in biotherapeutics and strengthen their role in basic medical applications.
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