Identification of candidate genes for aphid resistance in upland cotton by QTL mapping and expression analysis

IF 6 1区农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-10-01 DOI:10.1016/j.cj.2023.03.006

Qiushuang An , Zhenyuan Pan , Nurimanguli Aini , Peng Han , Yuanlong Wu , Chunyuan You , Xinhui Nie

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Abstract

Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants. Cotton plants are damaged by aphid (Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowledge on resistance genes and the mechanism. This study reported a lignin biosynthesis-related gene identified in the F₂ population derived from the cross between cotton cultivars Xinluzao 61 (resistant to aphid) and Xinluzao 50 (susceptible to aphid). A quantitative trait locus was mapped on chromosome D04 with a logarithm of odds (LOD) score of 5.99 and phenotypic effect of 27%. RNA-seq analysis of candidate intervals showed that the expression level of GH_D04G1418 was higher in the resistant cultivar than in the susceptible cultivar. This locus is close to AtLAC4 in the phylogenetic tree and contains a conserved laccase domain. Hence, it was designated GhLAC4-3. Silencing of GhLAC4-3 in Xinluzao 61 via virus-induced gene silencing (VIGS) resulted in decreased lignin content and increased susceptibility to aphids. These results suggest that GhLAC4-3 might enhance aphid resistance by regulating lignin biosynthesis in cotton.

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应用QTL定位和表达分析鉴定陆地棉抗蚜候选基因

木质素是细胞壁的主要成分之一，对植物抵抗害虫至关重要。棉蚜在世界范围内对棉花植株造成危害，但由于对棉蚜抗性基因和抗性机理的了解较少，抗性育种尚不成熟。本研究报道了从棉花品种新鲁早61（抗蚜）和新鲁早50（感蚜）杂交得到的F2群体中鉴定的一个木质素生物合成相关基因。在D04染色体上定位了一个数量性状基因座，其比值对数（LOD）得分为5.99，表型效应为27%。候选区间的RNA-seq分析表明，GH_D04G1418在抗性品种中的表达水平高于感病品种。该基因座在系统发育树中接近AtLAC4，并包含一个保守的漆酶结构域。因此，它被命名为GhLAC4-3。通过病毒诱导的基因沉默（VIGS）沉默新鲁早61中的GhLAC4-3导致木质素含量降低和对蚜虫的易感性增加。这些结果表明，GhLAC4-3可能通过调节棉花木质素的生物合成来增强对蚜虫的抗性。

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来源期刊

Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

9.90

自引率

3.00%

发文量

638

审稿时长

41 days

期刊介绍： The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.