Down-regulation of MeMYB2 leads to anthocyanin accumulation and increases chilling tolerance in cassava (Manihot esculenta Crantz)

IF 6 1区 农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-08-01 DOI:10.1016/j.cj.2023.03.009
Xin Guo , Xiaohui Yu , Chenyu Lin , Pingjuan Zhao , Bin Wang , Liangping Zou , Shuxia Li , Xiaoling Yu , Yinhua Chen , Peng Zhang , Ming Peng , Mengbin Ruan
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引用次数: 1

Abstract

Chilling-induced accumulation of reactive oxygen species (ROS) is harmful to plants, which usually produce anthocyanins to scavenge ROS as protection from chilling stress. As a tropical crop, cassava is hypersensitive to chilling, but the biochemical basis of this hypersensitivity remains unclear. We previously generated MeMYB2-RNAi transgenic cassava with increased chilling tolerance. Here we report that MeMYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside than the wild type under early chilling stress. Under this stress, the anthocyanin biosynthesis pathway was more active in MeMYB2-RNAi lines than in the wild type, and several genes involved in the pathway, including MeTT8, were up-regulated by MeMYB2-RNAi in the transgenic cassava. MeMYB2 bound to the MeTT8 promoter and blocked its expression under both normal and chilling conditions, thereby inhibiting anthocyanin accumulation. MeTT8 was shown to bind to the promoter of Dihydroflavonol 4-reductase (MeDFR-2) and increased MeDFR-2 expression. MeMYB2 appears to act as an inhibitor of chilling-induced anthocyanin accumulation during the rapid response of cassava to chilling stress.

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MeMYB2的下调导致木薯花青素的积累并提高其抗寒性(Manihot esculenta Crantz)
低温诱导的活性氧(ROS)积累对植物有害,植物通常会产生花青素来清除ROS,以抵御低温胁迫。作为一种热带作物,木薯对低温过敏,但这种过敏的生化基础尚不清楚。我们先前产生的MeMYB2 RNAi转基因木薯具有更高的抗寒性。在这里,我们报道了在早期冷胁迫下,MeMYB2 RNAi转基因木薯比野生型积累了更少的ROS,但更多的花青素3-O-葡萄糖苷。在这种胁迫下,MeMYB2 RNAi系中的花青素生物合成途径比野生型更活跃,并且在转基因木薯中,包括MeTT8在内的几个参与该途径的基因被MeMYB2 RNA上调。MeMYB2与MeTT8启动子结合,并在正常和冷藏条件下阻断其表达,从而抑制花青素积累。MeTT8显示与二氢黄酮醇4-还原酶(MeDFR-2)的启动子结合并增加MeDFR-2中的表达。在木薯对冷胁迫的快速反应过程中,MeMYB2似乎是冷诱导花青素积累的抑制剂。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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