Flavonol synthase gene MsFLS13 regulates saline-alkali stress tolerance in alfalfa

IF 6 1区 农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-08-01 DOI:10.1016/j.cj.2023.05.003
Lishuang Zhang , Yugang Sun , Jinqiang Ji , Weidi Zhao , Weileng Guo , Jiaqi Li , Yan Bai , Dan Wang , Zhe Yan , Changhong Guo
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引用次数: 1

Abstract

Alfalfa (Medicago sativa L.) is one of the most extensively grown leguminous forage worldwide. Environmental saline-alkali stress significantly influences the growth, development, and yield of alfalfa, posing a threat to its agricultural production. However, little is known about the potential mechanisms by which alfalfa responds to saline-alkali stress. Here, we investigated these mechanisms by cloning a saline-alkali-induced flavonol synthase gene (MsFLS13) from alfalfa, which was previously reported to be significantly upregulated under saline-alkali stress, and examining its function in the saline-alkali response. Overexpression of MsFLS13 in alfalfa promoted plant tolerance to saline-alkali stress by enhancing flavonol accumulation, antioxidant capacity, osmotic balance, and photosynthetic efficiency. Conversely, MsFLS13 inhibition using RNA interference reduced flavonol synthase activity and inhibited hairy root growth under saline-alkali stress. Yeast one-hybrid and dual-luciferase reporter assays indicated that the R2R3-MYB MsMYB12 transcription factor activates MsFLS13 expression by binding to the MBS motif in the MsFLS13 promoter. Further analysis revealed that abscisic acid mediates the saline-alkali stress response partially by inducing MsMYB12 and MsFLS13 expression, which consequently increases flavonol levels and maintains antioxidant homeostasis in alfalfa. Collectively, our findings highlight the crucial role of MsFLS13 in alfalfa in response to saline-alkali stress and provide a novel genetic resource for creating saline-alkali-resistant alfalfa through genetic engineering.

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黄酮合酶基因MsFLS13对苜蓿耐盐碱性的调控
苜蓿(Medicago sativa L.)是世界上种植最广泛的豆科牧草之一。环境盐碱胁迫严重影响苜蓿的生长发育和产量,对其农业生产构成威胁。然而,人们对苜蓿对盐碱胁迫的潜在反应机制知之甚少。在这里,我们通过从苜蓿中克隆一个盐碱诱导的黄酮醇合成酶基因(MsFLS13)来研究这些机制,该基因先前被报道在盐碱胁迫下显著上调,并检测其在盐碱反应中的功能。MsFLS13在苜蓿中的过表达通过增强黄酮醇积累、抗氧化能力、渗透平衡和光合效率来提高植物对盐碱胁迫的耐受性。相反,在盐碱胁迫下,使用RNA干扰的MsFLS13抑制降低了黄酮醇合酶活性并抑制了毛状根的生长。酵母单杂交和双荧光素酶报告基因分析表明,R2R3-MYB MsMYB12转录因子通过与MsFLS13启动子中的MBS基序结合来激活MsFLS13的表达。进一步的分析表明,脱落酸通过诱导MsMYB12和MsFLS13的表达部分介导盐碱胁迫反应,从而提高苜蓿中的黄酮醇水平并维持抗氧化稳态。总之,我们的研究结果突出了MsFLS13在苜蓿应对盐碱胁迫中的关键作用,并为通过基因工程创造耐盐碱苜蓿提供了新的遗传资源。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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