Identification of Green-Revertible Yellow 3 (GRY3), encoding a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase involved in chlorophyll synthesis under high temperature and high light in rice

IF 6 1区 农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-08-01 DOI:10.1016/j.cj.2023.02.001
Hongzhen Jiang , Anpeng Zhang , Banpu Ruan , Haitao Hu , Rui Guo , Jingguang Chen , Qian Qian , Zhenyu Gao
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引用次数: 2

Abstract

Chlorophyll, a green pigment in photosynthetic organisms, is generated by two distinct biochemical pathways, the tetrapyrrole biosynthetic pathway (TBP) and the methylerythritol 4-phosphate (MEP) pathway. MEP is one of the pathways for isoprenoid synthesis in plants, with 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) catalyzing its last step. In this study, we isolated a green-revertible yellow leaf mutant gry3 in rice and cloned the GRY3 gene, which encodes a HDR participating in geranylgeranyl diphosphate (GGPP) biosynthesis in chloroplast. A complementation experiment confirmed that a missense mutation (C to T) in the fourth exon of LOC_Os03g52170 causes the gry3 phenotype. Under high temperature and high light, transcript and protein abundances of GRY3 were reduced in the gry3 mutant. Transcriptional expression of chlorophyll biosynthesis, chloroplast development, and genes involved in photosynthesis were also affected. Excessive reactive oxygen species accumulation, cell death, and photosynthetic proteins degradation were occurred in the mutant. The content of GGPP was reduced in gry3 compared with Nipponbare, resulting in a stoichiometric imbalance of tetrapyrrolic chlorophyll precursors. These results shed light on the response of chloroplast biogenesis and maintenance in plants to high-temperature and high-light stress.

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绿色可还原黄3(GRY3)的鉴定,编码一种参与水稻高温高光下叶绿素合成的4-羟基-3-甲基丁-2-烯基二磷酸还原酶
叶绿素是光合生物中的一种绿色色素,由两种不同的生物化学途径产生,即四吡咯生物合成途径(TBP)和4-磷酸甲基赤藓糖醇(MEP)途径。MEP是植物类异戊二烯合成的途径之一,4-羟基-3-甲基丁-2-烯基二磷酸还原酶(HDR)催化其最后一步。在本研究中,我们在水稻中分离了一个绿色可回复的黄叶突变体gry3,并克隆了gry3基因,该基因编码参与叶绿体中香叶基-香叶基二磷酸(GGPP)生物合成的HDR。互补实验证实LOC_Os03g52170第四外显子中的错义突变(C到T)导致gry3表型。在高温和强光下,GRY3突变体中GRY3的转录物和蛋白质丰度降低。叶绿素生物合成、叶绿体发育和参与光合作用的基因的转录表达也受到影响。突变体中发生了过量的活性氧积累、细胞死亡和光合蛋白降解。与日本晴相比,gry3中GGPP的含量降低,导致四吡咯叶绿素前体的化学计量失衡。这些结果揭示了植物叶绿体的生物发生和维持对高温和高光胁迫的反应。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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