Di Wang , Syed Tatheer Alam Naqvi , Fanglin Lei , Zhenyu Zhang , Haiying Yu , Luyan Z. Ma
{"title":"Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads","authors":"Di Wang , Syed Tatheer Alam Naqvi , Fanglin Lei , Zhenyu Zhang , Haiying Yu , Luyan Z. Ma","doi":"10.1016/j.bioflm.2023.100155","DOIUrl":null,"url":null,"abstract":"<div><p>Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslG<sub>PA</sub>, from <em>Pseudomonas aeruginosa</em> was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from <em>Pseudomonas fluorescens</em>, PslG<sub>PF</sub>, exhibits anti-biofilm activities and the key catalytic residues of PslG<sub>PF</sub> are conserved with those of PslG<sub>PA</sub>. PslG<sub>PF</sub> at concentrations as low as 50 nM efficiently inhibits the biofilm formation of <em>P. aeruginosa</em> and disassemble its preformed biofilm. Furthermore, PslG<sub>PF</sub> exhibits anti-biofilm activity on a series of <em>Pseudomonads</em>, including <em>P. fluorescens, Pseudomonas stutzeri</em> and <em>Pseudomonas syringae</em> pv. <em>phaseolicola</em>. PslG<sub>PF</sub> stays active under various temperatures. Our findings suggest that <em>P. fluorescens</em> glycosyl hydrolase PslG<sub>PF</sub> has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of <em>Pseudomonads</em>.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":null,"pages":null},"PeriodicalIF":5.9000,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofilm","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590207523000527","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslGPA, from Pseudomonas aeruginosa was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from Pseudomonas fluorescens, PslGPF, exhibits anti-biofilm activities and the key catalytic residues of PslGPF are conserved with those of PslGPA. PslGPF at concentrations as low as 50 nM efficiently inhibits the biofilm formation of P. aeruginosa and disassemble its preformed biofilm. Furthermore, PslGPF exhibits anti-biofilm activity on a series of Pseudomonads, including P. fluorescens, Pseudomonas stutzeri and Pseudomonas syringae pv. phaseolicola. PslGPF stays active under various temperatures. Our findings suggest that P. fluorescens glycosyl hydrolase PslGPF has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of Pseudomonads.