Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2023-09-16 DOI:10.1016/j.bioflm.2023.100155
Di Wang , Syed Tatheer Alam Naqvi , Fanglin Lei , Zhenyu Zhang , Haiying Yu , Luyan Z. Ma
{"title":"Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads","authors":"Di Wang ,&nbsp;Syed Tatheer Alam Naqvi ,&nbsp;Fanglin Lei ,&nbsp;Zhenyu Zhang ,&nbsp;Haiying Yu ,&nbsp;Luyan Z. Ma","doi":"10.1016/j.bioflm.2023.100155","DOIUrl":null,"url":null,"abstract":"<div><p>Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslG<sub>PA</sub>, from <em>Pseudomonas aeruginosa</em> was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from <em>Pseudomonas fluorescens</em>, PslG<sub>PF</sub>, exhibits anti-biofilm activities and the key catalytic residues of PslG<sub>PF</sub> are conserved with those of PslG<sub>PA</sub>. PslG<sub>PF</sub> at concentrations as low as 50 nM efficiently inhibits the biofilm formation of <em>P. aeruginosa</em> and disassemble its preformed biofilm. Furthermore, PslG<sub>PF</sub> exhibits anti-biofilm activity on a series of <em>Pseudomonads</em>, including <em>P. fluorescens, Pseudomonas stutzeri</em> and <em>Pseudomonas syringae</em> pv. <em>phaseolicola</em>. PslG<sub>PF</sub> stays active under various temperatures. Our findings suggest that <em>P. fluorescens</em> glycosyl hydrolase PslG<sub>PF</sub> has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of <em>Pseudomonads</em>.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":null,"pages":null},"PeriodicalIF":5.9000,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofilm","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590207523000527","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslGPA, from Pseudomonas aeruginosa was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from Pseudomonas fluorescens, PslGPF, exhibits anti-biofilm activities and the key catalytic residues of PslGPF are conserved with those of PslGPA. PslGPF at concentrations as low as 50 nM efficiently inhibits the biofilm formation of P. aeruginosa and disassemble its preformed biofilm. Furthermore, PslGPF exhibits anti-biofilm activity on a series of Pseudomonads, including P. fluorescens, Pseudomonas stutzeri and Pseudomonas syringae pv. phaseolicola. PslGPF stays active under various temperatures. Our findings suggest that P. fluorescens glycosyl hydrolase PslGPF has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of Pseudomonads.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
荧光假单胞菌的糖基水解酶抑制假单胞菌生物膜的形成
生物膜是嵌入细胞外基质中的复杂微生物群落。生物膜内的病原体对抗生素的抵抗力比浮游生物更强。遇到生物膜需要新的策略。外多糖是生物膜基质的主要成分之一,在生物膜结构中起着至关重要的作用。在之前的研究中,来自铜绿假单胞菌的一种糖基水解酶PslGPA被发现能够通过分解生物膜中的胞外多糖来抑制生物膜的形成。在这里,我们研究了具有抗生物膜活性的PslG同源蛋白的潜在谱。荧光假单胞菌的一种糖基水解酶PslGPF具有抗生物膜活性,其关键催化残基与PslGPA的催化残基保守。低至50nM浓度的PslGPF有效抑制铜绿假单胞菌的生物膜形成并分解其预先形成的生物膜。此外,PslGPF对一系列假单胞菌表现出抗生物膜活性,包括荧光假单胞菌、stutzeri假单胞菌和丁香假单胞菌pv。Pharolicola。PslGPF在不同温度下保持活动。我们的研究结果表明,荧光假单胞菌糖基水解酶PslGPF有可能成为一种广谱的假单胞菌生物膜形成抑制剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
期刊最新文献
Transcatheter aortic valve implantation (TAVI) prostheses in vitro - biofilm formation and antibiotic effects Effects of nanoparticles on anaerobic, anammox, aerobic, and algal-bacterial granular sludge: A comprehensive review Identification and comparison of protein composition of biofilms in response to EGCG from Enterococcus faecalis and Staphylococcus lugdunensis, which showed opposite patterns in biofilm-forming abilities Cooperation between coagulase and von willebrand factor binding protein in Staphylococcus aureus fibrin pseudocapsule formation Collateral susceptibility-guided alternation of ceftolozane/tazobactam with imipenem prevents resistance development in XDR Pseudomonas aeruginosa biofilms
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1