Identification of a GC-rich leptin gene in chicken

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2016-08-01 DOI:10.1016/j.aggene.2016.04.001

Helena Farkašová, Tomáš Hron, Jan Pačes, Petr Pajer, Daniel Elleder

引用次数: 21

Abstract

Leptin (LEP) plays a key role in the regulation of energy balance in mammals, with less well-defined roles in lower vertebrates. The existence of LEP orthologs in birds has been controversial and only recently have several avian LEP genes been reported. Still, LEP has been assumed to be missing from the chicken (Gallus gallus) genome. Here, we identify the chicken LEP (cLEP), which shares only 25% amino acid sequence identity with its human ortholog. However, it has considerably higher homology with avian LEPs, and phylogenetic analysis shows it as clustering with avian LEP sequences. The cLEP and the other known avian LEP sequences all have high GC content. We argue that this property is behind the technical difficulties impeding the identification of these avian genes. The identification of cLEP can be expected to help elucidate leptin signaling pathways in wild and domestic chickens.

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鸡富含gc -瘦素基因的鉴定

瘦素(LEP)在哺乳动物的能量平衡调节中起着关键作用，但在低等脊椎动物中的作用不太明确。鸟类LEP同源基因的存在一直存在争议，直到最近才有几个鸟类LEP基因被报道。尽管如此，人们一直认为LEP在鸡(Gallus Gallus)基因组中缺失。在这里，我们鉴定了鸡LEP (cLEP)，它与人类同源物只有25%的氨基酸序列相同。但其与禽类LEP序列具有较高的同源性，系统发育分析表明其与禽类LEP序列呈聚类关系。cLEP和其他已知的禽类LEP序列均具有较高的GC含量。我们认为，这一特性背后的技术困难阻碍了这些鸟类基因的鉴定。cLEP的鉴定有望帮助阐明野生和家鸡瘦素信号通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.

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