Molecular characterization of a Se-containing glutathione peroxidases gene and its expressions to heavy metals compared with non-Se-containing glutathione peroxidases in Venerupis philippinarum

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2016-08-01 DOI:10.1016/j.aggene.2016.06.003
Ming Cong , Lei Zhang , Linbao Zhang , Jianmin Zhao , Huifeng Wu , Haiqiang Chen , Junli Kong
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引用次数: 5

Abstract

Heavy metal pollution is an increasing environmental problem around the coastline. As a kind of sedentary mollusk, Venerupis philippinarum is an important sentinel to survey the environmental quality of coastal flat. Glutathione peroxidases are important anti-oxidant enzymes to alleviate oxidative stress caused by heavy metals. In the present study, a new cDNA sequence encoding a Se-containing glutathione peroxidase (VpSeGPx2) was isolated from V. philippinarum. The full-length cDNA of VpSeGPx2 was 963 bp with a conserved selenocysteine insertion sequence (SECIS) in its 3′-UTR, encoding a polypeptide of 242 amino acids with a signal peptide of 19-amino acids. Phylogenetic analysis revealed that VpSeGPx2 was clustered with Se-GPx proteins from marine mollusks. VpSeGPx2 was found to be significantly down-regulated by 10 μg/L of copper and up-regulated by 40 μg/L of copper. However, cadmium exposure seemed to have no significant effect on the expression of VpSeGPx2 transcripts. As for a previously cloned non-Se-containing VpGPx, both concentrations of copper exposure (10 and 40 μg/L) significantly increased its mRNA expression, and a higher concentration (40 μg/L) of cadmium significantly inhibited the expression of VpGPx transcript. These results suggested that VpSeGPx2 and VpGPx were both involved in the detoxification of copper pollution but seemed to play a different role in cadmium pollution.

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菲律宾蛤含硒谷胱甘肽过氧化物酶基因的分子特征及其与非含硒谷胱甘肽过氧化物酶对重金属的表达比较
重金属污染是沿海地区日益严重的环境问题。菲律宾Venerupis philippinarum是一种定居软体动物,是调查滩地环境质量的重要哨兵。谷胱甘肽过氧化物酶是缓解重金属氧化应激的重要抗氧化酶。从菲律宾芽孢杆菌中分离到一个新的含硒谷胱甘肽过氧化物酶(VpSeGPx2) cDNA序列。VpSeGPx2全长963 bp,在其3 ' -UTR上有一个保守的硒代半胱氨酸插入序列(SECIS),编码242个氨基酸的多肽和19个氨基酸的信号肽。系统发育分析表明,VpSeGPx2与来自海洋软体动物的Se-GPx蛋白聚类。10 μg/L铜显著下调VpSeGPx2, 40 μg/L铜显著上调VpSeGPx2。然而,镉暴露似乎对VpSeGPx2转录本的表达没有显著影响。对于先前克隆的不含硒VpGPx,铜暴露浓度(10和40 μg/L)均显著提高了VpGPx mRNA的表达,镉暴露浓度(40 μg/L)显著抑制了VpGPx转录本的表达。这些结果表明,VpSeGPx2和VpGPx都参与了铜污染的解毒,但在镉污染中发挥的作用似乎不同。
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Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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