{"title":"Development of Transgenic Glyphosate-Resistant Rice with G6 Gene Encoding 5-Enolpyruvylshikimate-3-Phosphate Synthase","authors":"Te ZHAO, Chao-yang LIN, Zhi-cheng SHEN","doi":"10.1016/S1671-2927(11)60123-5","DOIUrl":null,"url":null,"abstract":"<div><p>Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glyphosate-resistant plants would increase the available choices for planting and lower the price of genetically modified crop seeds. A novel G6 gene from <em>Pseudomonas putida</em> that encoded 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was previously isolated. The G6 gene was transfected into rice <em>via Agrobacterium-mediated</em> transformation. The transgenic rice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmed that the transgenic rice can survive glyphosate spraying at a dose of 8 g L<sup>−1</sup>. In contrast, conventional rice was killed at a weed control glyphosate spray dose of 1 g L<sup>−1</sup>. Altogether, the present study showed that the G6 gene works well in rice <em>in vivo</em> for glyphosate-resistance.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1307-1312"},"PeriodicalIF":0.0000,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60123-5","citationCount":"39","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Sciences in China","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1671292711601235","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 39
Abstract
Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glyphosate-resistant plants would increase the available choices for planting and lower the price of genetically modified crop seeds. A novel G6 gene from Pseudomonas putida that encoded 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was previously isolated. The G6 gene was transfected into rice via Agrobacterium-mediated transformation. The transgenic rice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmed that the transgenic rice can survive glyphosate spraying at a dose of 8 g L−1. In contrast, conventional rice was killed at a weed control glyphosate spray dose of 1 g L−1. Altogether, the present study showed that the G6 gene works well in rice in vivo for glyphosate-resistance.
抗草甘膦作物在基因工程方面取得了巨大的经济成功。培育新的抗草甘膦植物将增加种植的可用选择，并降低转基因作物种子的价格。从恶臭假单胞菌中分离到一个编码5-烯醇丙酮酰莽草酸-3-磷酸合成酶(EPSPS)的新基因G6。通过农杆菌介导的转化将G6基因转染水稻。获得的转基因水稻经PCR、Southern和Western blot证实。室内试验和田间试验进一步证实，转基因水稻能在8 g L−1的草甘膦喷施下存活。相比之下，常规水稻在1 g L−1的除草剂草甘膦喷雾剂量下被杀死。综上所述，本研究表明G6基因在水稻体内具有良好的抗草甘膦能力。
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