Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

Agricultural Sciences in China Pub Date : 2011-12-01 DOI:10.1016/S1671-2927(11)60183-1

Wei JIANG, Shou-ping YANG, De-yue YU, Jun-yi GAI

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引用次数: 3

Abstract

Cytoplasmic male sterility plays an important role in utilization of crop heterosis. Screening of soybean for novel genes related to male sterility in soybean could provide a basis for studying the molecular mechanism of male sterility in plants. In this study, gene differential expressions between the cytoplasmic male-sterile line NJCMS1A and its maintainer line NJCMS1B in soybean were analyzed using cDNA-AFLP. A differentially expressed fragment, GmMF-T4A15, was isolated from large flower buds of NJCMS1B. By searching the soybean genomic library and PCR amplification, the cDNA full-length sequence of 1 311 bp was obtained and named GmMF1. The expression characteristics of GmMF1 were studied by semiquantitative real-time PCR and real-time quantitative PCR. The results showed that GmMF1 was expressed highly in flower buds of NJCMS1B. The deduced protein contains 436 amino acids and shows high similarity to members of the DUF620 protein family with unknown functions in other plant species. It is predicted that the protein encoded by GmMF1 is localized in the nucleus.

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大豆(Glycine max L. Merr.)新基因GmMF1的克隆与鉴定

摘要细胞质雄性不育在作物杂种优势利用中起着重要作用。大豆雄性不育相关新基因的筛选可为研究植物雄性不育的分子机制提供依据。本研究利用cDNA-AFLP分析了大豆细胞质雄性不育系NJCMS1A与其保持系NJCMS1B的基因表达差异。从NJCMS1B的大花蕾中分离到差异表达片段GmMF-T4A15。通过检索大豆基因组文库和PCR扩增，获得全长1 311 bp的cDNA序列，命名为GmMF1。采用半定量实时PCR和实时定量PCR研究GmMF1的表达特征。结果表明，GmMF1在NJCMS1B花蕾中高表达。该蛋白含有436个氨基酸，与DUF620蛋白家族成员高度相似，但在其他植物物种中功能未知。预测GmMF1编码的蛋白定位于细胞核内。

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来源期刊

Agricultural Sciences in China AGRICULTURE, MULTIDISCIPLINARY-

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3.2 months