Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

Agricultural Sciences in China Pub Date : 2011-12-01 DOI:10.1016/S1671-2927(11)60185-5

Shuai CHEN , Guan-shan LIU , Yuan-ying WANG , Yu-he SUN , Jia CHEN

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引用次数: 6

Abstract

Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca²⁺ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.

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钙依赖性蛋白激酶基因NtCDPK12的克隆及其在高盐和干旱条件下的诱导表达

摘要钙依赖性蛋白激酶(calcium -dependent protein kinase, CDPKs, EC 2.7.1.37)是植物中一个丝氨酸/苏氨酸激酶大家族，在植物Ca2+信号转导中起重要作用。利用cDNA末端快速扩增技术(RACE)从普通烟草(Nicotiana tabacum)叶片中分离到全长CDPK基因NtCDPK12 (GenBank登录号GQ337420)。NtCDPK12 cDNA全长1 816 bp，包含1 461 bp的开放阅读框(ORF)，编码486个氨基酸。序列比对表明，NtCDPK12包含CDPKs中发现的所有保守区域，并且与许多其他植物CDPKs显示出高度的序列相似性。实时定量反转录pcr (qRT-PCR)结果显示，NtCDPK12在高盐胁迫和干旱胁迫下的茎中高表达，在根系中表达量增加，表明NtCDPK12是在高盐胁迫和干旱胁迫下诱导的。

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Agricultural Sciences in China AGRICULTURE, MULTIDISCIPLINARY-

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3.2 months