Deng-xia YI, Lei CUI, Yu-mei LIU, Mu ZHUANG, Yang-yong ZHANG, Zhi-yuan FANG, Li-mei YANG
{"title":"Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth","authors":"Deng-xia YI, Lei CUI, Yu-mei LIU, Mu ZHUANG, Yang-yong ZHANG, Zhi-yuan FANG, Li-mei YANG","doi":"10.1016/S1671-2927(11)60167-3","DOIUrl":null,"url":null,"abstract":"<div><h3>Abstract</h3><p>To obtain transgenic cabbage line with broad insect resistance, a new synthetic <em>Bacillus thuringiensis cry1Ba3</em> gene was introduced into white cabbage <em>via Agrobacterium tumefaciens</em>-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that <em>cry1Ba3</em> was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that <em>cry1Ba3</em> was expressed. Western blot results confirmed the production of insecticidal protein encoded by <em>cry1Ba3</em>. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1693-1700"},"PeriodicalIF":0.0000,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60167-3","citationCount":"27","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Sciences in China","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1671292711601673","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 27
Abstract
To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.
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