Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage

Agricultural Sciences in China Pub Date : 2011-11-01 DOI:10.1016/S1671-2927(11)60164-8

Biao-lin HU , Xue-qin FU , Tao ZHANG , Yong WAN , Xia LI , Yun-hong HUANG , Liang-fang DAI , Xiang-dong LUO , Jian-kun XIE

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引用次数: 12

Abstract

Drought stress is one of the major constraints to rice (Oryza sativa L.) production and yield stability especially in rainfed ecosystems and is getting worse as the climate changes worldwide. Dongxiang wild rice (DXWR) Oryza rufipogon Griff., contains drought resistant gene. Improving drought resistance of cultivars is crucial to increase and stabilize rice grain yield via transferring resistant gene from species related to rice. In this paper, four upland rice, sixty backcross inbred lines (BILs) derived from BC₁F₅ of R974//DXWR/R974, and their parents were employed to evaluate drought-resistance at seedling stage in the greenhouse. Nine traits were recorded for assessment of drought resistance, including maximum root length (MRL), number of roots (NR), shoot length (SL), dry root weight (DRW), fresh root weight (FRW), root relative water content (RRWC), leaf relative water content (LRWC), level for rolling leaf (LRL), and seedling survivability under repeat drought (SSRD). Using more than 88% of accumulative contribution resulted from the principal component analysis (PCA), the nine traits were classified into five independent principal components and the line 1949 showed the highest resistance. Analysis on the stepwise regression equation and correlation demonstrated that MRL, RN, FRW, and RRWC significantly influenced the drought resistance, thus could be used as comprehensive index for drought resistance at the seedling stage. Using the major gene plus polygene mixed inheritance model of quantitative traits, the inheritance of drought-resistance of BIL population at seedling stage was mostly controlled by two independent genes plus polygene. As a result, the DXWR could be precious resources for genetic improvement of drought resistance in cultivated rice.

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东乡野生稻及其衍生回交自交系苗期抗旱性测定性状的遗传分析

摘要干旱胁迫是制约水稻(Oryza sativa L.)生产和产量稳定的主要因素之一，特别是在雨养生态系统中，随着全球气候变化，干旱胁迫日益严重。东乡野生稻(DXWR)。，含有抗旱基因。通过转移水稻相关品种的抗旱性基因，提高水稻品种的抗旱性是提高和稳定水稻产量的关键。以4个旱稻、60个由R974//DXWR/R974品种BC1F5衍生的回交自交系(BILs)及其亲本为材料，在温室内进行了苗期抗旱性评价。通过记录最大根长(MRL)、根数(NR)、茎长(SL)、干根重(DRW)、鲜根重(FRW)、根相对含水量(RRWC)、叶相对含水量(LRWC)、滚叶水平(LRL)和幼苗抗旱性(SSRD)等9个性状来评价其抗旱性。利用主成分分析(PCA)的累积贡献率超过88%，将9个性状划分为5个独立的主成分，以1949系抗性最高。逐步回归方程和相关分析表明，MRL、RN、FRW和RRWC对苗期抗旱性影响显著，可作为苗期抗旱性的综合指标。利用数量性状的主基因加多基因混合遗传模式，油菜群体苗期抗旱性遗传主要由两个独立基因加多基因控制。因此，DXWR可作为栽培水稻抗旱性遗传改良的宝贵资源。

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Agricultural Sciences in China AGRICULTURE, MULTIDISCIPLINARY-

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3.2 months