Isolation, Identification of Bacillus Thuringiensis/ Cereus and Its Enhancement on Protein Wastewater Treatment by Rhodobacter Sphaeroides

Liu Shuli, Zhang Guangming, Zhang Jie
{"title":"Isolation, Identification of Bacillus Thuringiensis/ Cereus and Its Enhancement on Protein Wastewater Treatment by Rhodobacter Sphaeroides","authors":"Liu Shuli, Zhang Guangming, Zhang Jie","doi":"10.11916/J.ISSN.1005-9113.2016.05.010","DOIUrl":null,"url":null,"abstract":"In order to enhance the degrading protein capability of purple non-sulfur bacteria (PNSB), an effective strain, L2, was used to co-culture with Rhodobacter sphaeroides ATCC17023. The effects of added strain on protein removal of R. sphaeroides were investigated. Results showed that strain L2, being identified as Bacillus thuringiensis / cereus , had a high potential for producing protease with a production of 295 U/mL.The optimal B. thuringiensis / cereus (40 μL) could significantly increase protein degradation of R. sphaeroides . Protein removal and biomass production were improved by 483% and 67%, respectively. R. sphaeroides /total biomass production was more than 95%. Theoretical analysis revealed that R. sphaeroides syntrophically interacted with B. thuringiensis / cereus . Protein degradation of B. thuringiensis / cereus provided small molecule substrates (VFAs) for R. sphaeroides growth and cells materials synthesis.","PeriodicalId":39923,"journal":{"name":"Journal of Harbin Institute of Technology (New Series)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Harbin Institute of Technology (New Series)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11916/J.ISSN.1005-9113.2016.05.010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Engineering","Score":null,"Total":0}
引用次数: 0

Abstract

In order to enhance the degrading protein capability of purple non-sulfur bacteria (PNSB), an effective strain, L2, was used to co-culture with Rhodobacter sphaeroides ATCC17023. The effects of added strain on protein removal of R. sphaeroides were investigated. Results showed that strain L2, being identified as Bacillus thuringiensis / cereus , had a high potential for producing protease with a production of 295 U/mL.The optimal B. thuringiensis / cereus (40 μL) could significantly increase protein degradation of R. sphaeroides . Protein removal and biomass production were improved by 483% and 67%, respectively. R. sphaeroides /total biomass production was more than 95%. Theoretical analysis revealed that R. sphaeroides syntrophically interacted with B. thuringiensis / cereus . Protein degradation of B. thuringiensis / cereus provided small molecule substrates (VFAs) for R. sphaeroides growth and cells materials synthesis.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
苏云金芽孢杆菌/蜡样芽孢杆菌的分离鉴定及其对球形红杆菌处理蛋白质废水的强化作用
为了提高紫色无硫细菌(PNSB)降解蛋白质的能力,采用有效菌株L2与球形红杆菌ATCC17023共培养。研究了添加菌株对球孢霉蛋白去除的影响。结果表明,菌株L2为苏云金芽孢杆菌/蜡样芽孢杆菌,具有较高的蛋白酶生产潜力,产量为295 U/mL。最优的苏云金芽孢杆菌/蜡状芽孢杆菌浓度(40 μL)能显著提高球藻蛋白的降解率。蛋白质去除率和生物量产量分别提高了483%和67%。球藻生物量占总生物量的比例在95%以上。理论分析表明,圆叶红与苏云金芽孢杆菌/蜡状芽孢杆菌之间存在着同养相互作用。苏云金芽孢杆菌/蜡状芽孢杆菌的蛋白质降解为圆球孢子虫生长和细胞材料合成提供了小分子底物(VFAs)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
0.50
自引率
0.00%
发文量
2515
期刊最新文献
Numerical simulation of nitrogen and phosphorus vertical transportation in subsurface drainage area Design of Sixth-Order Lowpass Elliptic Switched-Capacitor Filter Model-free Adaptive Control for Spacecraft Attitude Type Synthesis of fully-Decoupled 2T2R Parallel Mechanisms Based on Driven-Chain Principle Isolation, Identification of Bacillus Thuringiensis/ Cereus and Its Enhancement on Protein Wastewater Treatment by Rhodobacter Sphaeroides
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1