EFFECT OF DIFFERENT REMEDIATION METHODS ON THE DEGRADATION RATE OF PETROLEUM HYDROCARBON AND ENZYME ACTIVITY IN PETROLEUM CONTAMINATED SOIL

Abstract

. In order to explore the effects of different remediation methods on the degradation rate of total petroleum hydrocarbons and enzyme activity in oil-contaminated soil, a study was conducted using six different treatments, including adding rhamnolipid (S), organic fertilizer (F), degradation bacteria (J), rhamnolipid + degrading bacteria (SJ), organic fertilizer + rhamnolipid (SF), and organic fertilizer + degradation bacteria (FJ), to remediate the oil-contaminated soil. The study examined the changes in the degradation rate of total petroleum hydrocarbons and the activity of four soil enzymes (urease, peroxidase, dehydrogenase, and lipase) at different cultivation times. The results showed that after 60 days of remediation, all treatments improved the degradation rate of total petroleum hydrocarbons in the contaminated soil. The best result was achieved with the FJ treatment, with a degradation rate of 31.72%. The enzyme activity in all treatments was significantly higher than that of the control at different cultivation periods. Statistical analysis showed that the activity of urease, peroxidase, and lipase was significantly negatively correlated with the residual rate of total petroleum hydrocarbons in the contaminated soil. The activity of dehydrogenase was highly significantly negatively correlated with the residual rate of total petroleum hydrocarbons in the contaminated soil.