Fractional Composition of Protein in Blood Plasma of Carp (Cyprinus carpio) under the Aliphatic Amines

Abstract

The effect of different concentrations of methylamine and propylamine in water on content and fractional composition of plasma proteins of the two-year-old carp (Cyprinus carpio L.) was studied. The total protein content in the blood plasma carp increased under methylamine concentration 0.009 mg/dm3, whereas its concentration 0.005 and 0.007 mg/dm3 and all considered concentration of propylamine (0.04, 0.06 and 0.08 mg/dm3) did not affect total protein content. The main portion of proteins in the carp's blood plasma was formed by proteins of the molecular weight 52-54, 68-72 and 90 kDa, whereas content of proteins of the molecular weight 35, 80, 100, 150-170, 340 and 900 kDa was notably lower. After 72 h of exposition under different methylamine concentration, content of proteins of the molecular weight 25, 90 and 100 kDa increased, at the same time content of proteins of the molecular weight 35-52, 150-170 and 180-190 kDa decreased, and content of other proteins did not change. Changes of the proteins' fractional composition under the propylamine impact depended on its concentration and consisted in elevated content of proteins of the molecular weight 90 and 100 kDa, lower content of the fractions 150-170 and 180-190 kDa, and constant content of other fractions.