Study of CFTR channel functional activity in a patient with cystic fibrosis and E92K/4428insGA genotype

A. Efremova, V. Sherman, Y. Melyanovskaya, N. Bulatenko, T. Bukharova, D. Mokrousova, O. Makhnach, D. Goldshtein, E. Kondratyeva
{"title":"Study of CFTR channel functional activity in a patient with cystic fibrosis and E92K/4428insGA genotype","authors":"A. Efremova, V. Sherman, Y. Melyanovskaya, N. Bulatenko, T. Bukharova, D. Mokrousova, O. Makhnach, D. Goldshtein, E. Kondratyeva","doi":"10.20953/1727-5784-2022-5-72-79","DOIUrl":null,"url":null,"abstract":"Clinical manifestations of cystic fibrosis depend on the patient’s genotype since there are more than 2,000 variants of the CFTR gene. Main complications of the disease are expressed in a reduction or loss of pancreatic function and bacterial lung infection, leading to respiratory failure. The E92K (p.(Glu92Lys) and 4428insGA (p.(Ser1435Glyfs*14) mutations in the CFTR gene are accompanied by a high frequency of preserved pancreatic function. In a 3-year-old patient with recurrent pancreatitis and E92K/4428insGA genotype, the functional activity of the CFTR channel was studied using the intestinal current measurement method on rectal biopsy specimens and the forskolin-induced swelling assay in intestinal organoids. The personalized assessment of the action of CFTR modulators was also performed on organoid cultures obtained from the patient’s intestinal biopsies. In a 3-year-old child with cystic fibrosis and recurrent pancreatitis with frequent exacerbations, a high preservation of residual CFTR channel function was revealed. All the investigated targeted drugs were effective in increasing the functional activity of the CFTR chloride channel. Key words: cystic fibrosis, CFTR gene, targeted therapy, pancreatitis, intestinal organoids, intestinal current measurement, E92K, 4428insGA","PeriodicalId":53444,"journal":{"name":"Voprosy Detskoi Dietologii","volume":"7 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Voprosy Detskoi Dietologii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20953/1727-5784-2022-5-72-79","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Clinical manifestations of cystic fibrosis depend on the patient’s genotype since there are more than 2,000 variants of the CFTR gene. Main complications of the disease are expressed in a reduction or loss of pancreatic function and bacterial lung infection, leading to respiratory failure. The E92K (p.(Glu92Lys) and 4428insGA (p.(Ser1435Glyfs*14) mutations in the CFTR gene are accompanied by a high frequency of preserved pancreatic function. In a 3-year-old patient with recurrent pancreatitis and E92K/4428insGA genotype, the functional activity of the CFTR channel was studied using the intestinal current measurement method on rectal biopsy specimens and the forskolin-induced swelling assay in intestinal organoids. The personalized assessment of the action of CFTR modulators was also performed on organoid cultures obtained from the patient’s intestinal biopsies. In a 3-year-old child with cystic fibrosis and recurrent pancreatitis with frequent exacerbations, a high preservation of residual CFTR channel function was revealed. All the investigated targeted drugs were effective in increasing the functional activity of the CFTR chloride channel. Key words: cystic fibrosis, CFTR gene, targeted therapy, pancreatitis, intestinal organoids, intestinal current measurement, E92K, 4428insGA
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
囊性纤维化E92K/4428insGA基因型患者CFTR通道功能活性的研究
囊性纤维化的临床表现取决于患者的基因型,因为CFTR基因有2000多种变体。该疾病的主要并发症表现为胰腺功能降低或丧失和细菌性肺部感染,导致呼吸衰竭。CFTR基因中的E92K (p.(Glu92Lys))和4428insGA (p.(Ser1435Glyfs*14))突变伴随着高频率的胰腺功能保留。对1例3岁复发性胰腺炎E92K/4428insGA基因型患者,采用直肠活检标本肠电流测量法和肠道类器官福斯克林诱导肿胀法研究CFTR通道的功能活性。对CFTR调节剂作用的个性化评估也对患者肠道活检获得的类器官培养物进行了评估。在一个患有囊性纤维化和复发性胰腺炎的3岁儿童中,发现了残留CFTR通道功能的高度保存。所研究的靶向药物均能有效提高CFTR氯离子通道的功能活性。关键词:囊性纤维化,CFTR基因,靶向治疗,胰腺炎,肠道类器官,肠电流测量,E92K, 4428insGA
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Voprosy Detskoi Dietologii
Voprosy Detskoi Dietologii Medicine-Pediatrics, Perinatology and Child Health
CiteScore
1.20
自引率
0.00%
发文量
17
期刊介绍: The scientific journal Voprosy Detskoi Dietologii is included in the Scopus database. Publisher country is RU. The main subject areas of published articles are Food Science, Pediatrics, Perinatology, and Child Health, Nutrition and Dietetics, Клиническая медицина.
期刊最新文献
Computational design of soluble functional analogues of integral membrane proteins. Malnutrition in children with cystic fibrosis and its correction with a specialized formula Eosinophilic esophagitis: clinical presentation, current aspects of diagnosis and treatment Role of functional nutrition in reducing the incidence of acute respiratory infections in children Influence of feeding type on gut microbiome in children and adolescents
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1