THE IMPACT OF ARTIFICIAL GRAVITY MODELLED IN SHORT-ARM CENTRIFUGE ON THE EXPRESSION OF TLR-ASSOCIATED GENES OF INNATE IMMUNITY

Abstract

Here, we report the changes in the expression of TLR-associated genes, genes of NF-kB, AP-1, and TLR4 pathways, genes coding for apoptotic proteins, cytokines, and chemokines, after rotation on the short-arm centrifuge. The study involved 10 almost healthy male volunteers aged 25-40. CD14+ monocytes were isolated from the testers’ blood before and after the exposure to the artificial gravity. The cells were incubated with TLR1-9 ligands for 24 hours to assess the monocytes’ reserve potential. The gene expression was evaluated by real-time PCR. In sum, no negative effect of rotation on the short-arm centrifuge on the functioning of TLR-associated branch of innate immunity was detected.