EFFECTS OF 3-DAY IMMERSION ON BLOOD PROTEOME IN FEMALE VOLUNTEERS

Abstract

Chromatography-mass spectrometry was used to analyze dried blood spots collected from 6 female volunteers in a 3-d dry immersion (DI) in order to detect proteins involved in acute adaptation of the female physiological systems. Sampling was performed before DI, every day in DI and on the next day after DI completion. Proteomic analysis evidenced an immediate reaction of proteins involved in carbohydrate metabolism and proteasome degradation of proteins. On DI day-2, we saw a reaction of immune response proteins and decreased PEDF level, an angiogenesis inhibitor that controls many processes, including heart protection from injuries. On DI-3, the response was joined by proteins participating in processes of the complement cascade and coagulation, platelet degranulation, hemostasis, extracellular matrix formation, glycolysis, water-salt balance, antioxidant system, and the neutrophils involving defense response. It should be noted that levels of histidine triad 1 nucleotide-binding protein 1 (HINT1) and myosin light chain 4 (MYL4) that play an important role in cardiac adaptation to a volume overload were found increased on DI day-3. Two days after DI, proteins did not all had returned to baseline levels. Changes in the level of proteins involved in lipid metabolism were observed only after DI completion.