V. Tadić, A. Zugic, S. Djordjevic, I. Zizović, I. Homšek, D. Mišić, I. Nešić
{"title":"The RP-HPLC method for analysis of usnic acid as potential marker of herbal drugs-based formulations containing Usnea barbata","authors":"V. Tadić, A. Zugic, S. Djordjevic, I. Zizović, I. Homšek, D. Mišić, I. Nešić","doi":"10.2298/jsc201216045t","DOIUrl":null,"url":null,"abstract":"The aim of this study was to develop and validate sensitive RP-HPLC method for determination of usnic acid, as potential marker substance of the herbal product for oromucosal use being the complex mixture of plant extracts and an essential oil, including the extract of Usnea barbata. Analysis of usnic acid in the tested formulation was performed through an extraction with methanol, prior to injection on the HPLC column (Zorbax Eclipse XDB-C18 600 Bar (4.6?100 mm, 1.8 ?m)). Employed gradient procedure of the solvents (phosphoric acid (pH 2.5) and acetonitrile), at flow rate of 0.1 mL/min allowed efficient and reproducible separation of usnic acid from other compounds present in the investigated complex mixture. The established suitability, linearity, precision, accuracy and selectivity/specificity of this assay implied its applicability as a reliable examination in the stability study of the investigated multi-ingredient herbal preparation.","PeriodicalId":17489,"journal":{"name":"Journal of The Serbian Chemical Society","volume":"1 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The Serbian Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.2298/jsc201216045t","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 3
Abstract
The aim of this study was to develop and validate sensitive RP-HPLC method for determination of usnic acid, as potential marker substance of the herbal product for oromucosal use being the complex mixture of plant extracts and an essential oil, including the extract of Usnea barbata. Analysis of usnic acid in the tested formulation was performed through an extraction with methanol, prior to injection on the HPLC column (Zorbax Eclipse XDB-C18 600 Bar (4.6?100 mm, 1.8 ?m)). Employed gradient procedure of the solvents (phosphoric acid (pH 2.5) and acetonitrile), at flow rate of 0.1 mL/min allowed efficient and reproducible separation of usnic acid from other compounds present in the investigated complex mixture. The established suitability, linearity, precision, accuracy and selectivity/specificity of this assay implied its applicability as a reliable examination in the stability study of the investigated multi-ingredient herbal preparation.
期刊介绍：
The Journal of the Serbian Chemical Society -JSCS (formerly Glasnik Hemijskog društva Beograd) publishes articles original papers that have not been published previously, from the fields of fundamental and applied chemistry:
Theoretical Chemistry, Organic Chemistry, Biochemistry and Biotechnology, Food Chemistry, Technology and Engineering, Inorganic Chemistry, Polymers, Analytical Chemistry, Physical Chemistry, Spectroscopy, Electrochemistry, Thermodynamics, Chemical Engineering, Textile Engineering, Materials, Ceramics, Metallurgy, Geochemistry, Environmental Chemistry, History of and Education in Chemistry.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
