{"title":"Binding interactions of actinomycin D anticancer drug with bile salts micelles","authors":"A. Toader, I. Dascalu, E. Neacsu, M. Enache","doi":"10.2298/jsc221102004t","DOIUrl":null,"url":null,"abstract":"The interactions of actinomycin D (ActD) anticancer drug with two bile salts having different hydrophobicity (sodium cholate (NaC) and sodium deoxycolate (NaDC)) and the influence of these bile salts aggregates on the (ActD-DNA) complex was investigated in 10 mM phosphate buffer (pH 7.4) by UV-Vis spectroscopy (absorption and thermal denaturation). The binding strength of ActD to NaDC is higher than for NaC, and this difference attests stronger hydrophobic interactions between ActD and NaDC micelles. Also, the partition coefficient is significantly higher for NaDC micelles than NaC micelles, in line with larger aggregates formed by NaDC. The spectral profile of actinomycin D molecules into NaC and NaDC micelles in comparison with different solvents implies that actinomycin D molecule experiences a hydrophobic environment in bile salts aggregates. Regarding the influence of NaC and NaDC aggregates on the (ActD-DNA) complex, it was shown that the presence of both bile salts micelles do not induce the deintercalation of actinomycin D molecules from DNA duplex.","PeriodicalId":17489,"journal":{"name":"Journal of The Serbian Chemical Society","volume":"1 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The Serbian Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.2298/jsc221102004t","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 2
Abstract
The interactions of actinomycin D (ActD) anticancer drug with two bile salts having different hydrophobicity (sodium cholate (NaC) and sodium deoxycolate (NaDC)) and the influence of these bile salts aggregates on the (ActD-DNA) complex was investigated in 10 mM phosphate buffer (pH 7.4) by UV-Vis spectroscopy (absorption and thermal denaturation). The binding strength of ActD to NaDC is higher than for NaC, and this difference attests stronger hydrophobic interactions between ActD and NaDC micelles. Also, the partition coefficient is significantly higher for NaDC micelles than NaC micelles, in line with larger aggregates formed by NaDC. The spectral profile of actinomycin D molecules into NaC and NaDC micelles in comparison with different solvents implies that actinomycin D molecule experiences a hydrophobic environment in bile salts aggregates. Regarding the influence of NaC and NaDC aggregates on the (ActD-DNA) complex, it was shown that the presence of both bile salts micelles do not induce the deintercalation of actinomycin D molecules from DNA duplex.
期刊介绍：
The Journal of the Serbian Chemical Society -JSCS (formerly Glasnik Hemijskog društva Beograd) publishes articles original papers that have not been published previously, from the fields of fundamental and applied chemistry:
Theoretical Chemistry, Organic Chemistry, Biochemistry and Biotechnology, Food Chemistry, Technology and Engineering, Inorganic Chemistry, Polymers, Analytical Chemistry, Physical Chemistry, Spectroscopy, Electrochemistry, Thermodynamics, Chemical Engineering, Textile Engineering, Materials, Ceramics, Metallurgy, Geochemistry, Environmental Chemistry, History of and Education in Chemistry.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
