In vivo Neuroprotective Activity of the Chloroform Selective Extract from the Bark of Erythrina senegalensis DC. (Fabaceae) and Characterization of two Isolated Phytoconstituents

Abstract

The present study aims to isolate and identify bioactive phytocompounds derived from the crude methanolic extract, an effective extract of Erythrina senegalensis DC. (Fabaceae). The chloroform extract (selective extract of the crude methanol extract) was obtained by liquidliquid extraction, then tested for its protective activity in vivo against Alzheimer's disease in a mouse model. After showing a beneficial effect, this extract underwent chromatographic (open column, HPLC) and spectroscopic (NMR) analyzes in order to isolate and characterize the phytocompounds which would be at the origin of the observed biological activities. Thus, two phycompounds of the flavonoid class: osajin and cajaflavanone, were isolated for the first time from E. senegalensis from Côte d'Ivoire.