Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES Acta Veterinaria Brno Pub Date : 2022-01-01 DOI:10.2754/avb202291030235

E. Karakurt, E. Beytut, S. Dağ, H. Nuhoğlu, A. Yıldız, Emre Kurtbaş

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引用次数: 1

Abstract

This study aimed to reveal the presence of reactive oxygen species (ROS)-induced lipid peroxidation and DNA damage in ovine pulmonary adenocarcinomas (OPA) by evaluating malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expressions by immunohistochemical and immunofluorescence methods. Lung tissue samples were collected from 26 sheep brought to the Pathology Department for routine diagnosis. Lung tissues were fixed in 10% neutral buffered formalin, following routine procedures tissues were stained with hematoxylin and eosin. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Indirect immunofluorescence method was applied to the sections. Tumoral cells showed acinar, papillary or mixed type patterns. Only 2 of 20 cases metastasized to regional lymph nodes. All OPAs were immune positive for Jaagsiekte Sheep Retrovirus Capsid Protein (JSRV CA), MDA and 8-OHdG. The control group was negative for JSRV CA, MDA and 8-OHdG expressions. Malondialdehyde and 8-OHdG immune positive cells were statistically increased in the OPA group compared to the control group. In conclusion, our results demonstrate that higher MDA and 8-OHdG expressions in sheep with OPA suggest that OPA may be closely related to lipid peroxidation and oxidative DNA damage.

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免疫组织化学和免疫荧光法检测羊肺腺癌组织中MDA和8-OHdG的表达

本研究旨在通过免疫组织化学和免疫荧光法检测丙二醛(MDA)和8-羟基-2-脱氧鸟苷(8-OHdG)在羊肺腺癌(OPA)组织中的表达，揭示活性氧(ROS)诱导的脂质过氧化和DNA损伤的存在。对送到病理科进行常规诊断的26只羊采集肺组织样本。肺组织固定在10%中性缓冲福尔马林中，按常规程序用苏木精和伊红染色。免疫组织化学染色采用亲和素-生物素过氧化物酶法。切片采用间接免疫荧光法。肿瘤细胞呈腺泡状、乳头状或混合型。20例中仅有2例转移到局部淋巴结。所有OPAs对Jaagsiekte羊逆转录病毒衣壳蛋白(JSRV CA)、MDA和8-OHdG均免疫阳性。对照组JSRV CA、MDA、8-OHdG表达均为阴性。与对照组相比，OPA组丙二醛和8-OHdG免疫阳性细胞有统计学意义增加。综上所述，我们的研究结果表明，OPA绵羊体内MDA和8-OHdG的高表达表明OPA可能与脂质过氧化和DNA氧化损伤密切相关。

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.