Investigation of Serum Anti-Mullerian Hormone Levels at Follicular Phase and Interestrus Period in Queens

Abstract

The objectives of this study were to report mean serum AMH concentrations in adult queens, to determine relationship between AMH concentrationss with estradiol level, to investigate the relationship between serum AMH with follicular phase and interestrus period and to discuss the usability of AMH as a biomarker to diagnosis of ovarian remnant syndrome. In the study, 64 adult and healthy female queen, who were brought to clinics for ovariohysterectomy were used. After anamnesis, vaginal cytology, macroscopic examination of the uterus and ovaries and estradiol measurement, queens were divided into two groups: follicular phase (n: 45) and interestrus (n: 19). Estradiol and AMH levels in serum samples were determined by ELISA. In the evaluation of serum AMH concentrations, a statistical difference was determined between the follicular phase and interestrus (p˂0.05). A negative correlation was found between age and estradiol in both follicular phase (r = -0.171) and interestrus (r = -0.385) groups. A positive correlation was found between age and AMH in the interestrus (p˂0.01, r = 0.696). Serum AMH levels in the interestrus were found to be significantly higher than follicular phase (p˂0.05). It was thought that this increase in serum AMH levels during the interestrus period, which is the stage where oocytes were selected for ovulation, may be an indicator of the role of AMH in oocyte selection in queens, as in many other mammalian species.