Χαλλούμι (Halloumi)/Hellim is the traditional cheese of Cy¬prus and belongs to whole of divided Island with Turkish and Greek name. Especially with the Commission Implementing Regulation (EU) 2021/591 of 12 April 2021 that entering a name in the register of protected designations of origin and protected geographical indications (‘Χαλλούμι’ (Halloumi)/‘Hellim’, the spotlights are more on the product nowadays. Commercial samples of fresh and mature halloumi cheeses made from sheep/goat, bovine or both milk were examined to evaluate their physicochemical and microbiological properties. In physicochemical analysis, potential of hydrogen (pH), titratable acidity (TA), salt-sodium chloride (NaCl), dry-matter (DM) ratios were analyzed. While examining the quality and safety indicators for microbiological analysis; total mesophilic aerob bacteria (TMAB), yeast and molds, lactic acid bacteria (LAB), coliform bacteria, coagulase-positive staphylococci, Listeria monocytogenes, Salmonella spp. analyzes were made. This study will help establish the quality profile of halloumi cheese and identify potential hazards and sources of contamination.
{"title":"Investigation of Quality Characteristics of Industrially Produced Halloumi Cheese","authors":"Beyza Hatice ULUSOY, Fatma KAYA, Doruk KAYNARCA, Şifa BERKAN, Hafizu İbrahim KADEMİ, Canan HECER","doi":"10.33988/auvfd.1218926","DOIUrl":"https://doi.org/10.33988/auvfd.1218926","url":null,"abstract":"Χαλλούμι (Halloumi)/Hellim is the traditional cheese of Cy¬prus and belongs to whole of divided Island with Turkish and Greek name. Especially with the Commission Implementing Regulation (EU) 2021/591 of 12 April 2021 that entering a name in the register of protected designations of origin and protected geographical indications (‘Χαλλούμι’ (Halloumi)/‘Hellim’, the spotlights are more on the product nowadays. Commercial samples of fresh and mature halloumi cheeses made from sheep/goat, bovine or both milk were examined to evaluate their physicochemical and microbiological properties. In physicochemical analysis, potential of hydrogen (pH), titratable acidity (TA), salt-sodium chloride (NaCl), dry-matter (DM) ratios were analyzed. While examining the quality and safety indicators for microbiological analysis; total mesophilic aerob bacteria (TMAB), yeast and molds, lactic acid bacteria (LAB), coliform bacteria, coagulase-positive staphylococci, Listeria monocytogenes, Salmonella spp. analyzes were made. This study will help establish the quality profile of halloumi cheese and identify potential hazards and sources of contamination.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136360674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hidayet TUTUN, Volkan İPEK, Musa TATAR, Ayşe KIZILYER, Muhammet Mükerrem KAYA
G Protein-Coupled Receptors, GPR55 and GPR119 are widely distributed throughout the body and exert important biological functions. However, little is known about their roles in testis. This study aimed to examine the expression and distribution of GPR55 and GPR119 during the development of the rat testis. Sixty male Sprague Dawley rats (180–240 g) were divided into 10 groups as 7, 14, 21, 28, 35, 42, 49, 56, 63, and 70 postnatal days of age (PND) (six animals per group). The testicular expression of GPR55 and GPR119 has been investigated by immunohistochemistry, Western blot, and quantitative RT-PCR methods. We observed that GPR55 and GPR119 are expressed throughout the rat testis development from PND 7 to 70. However, no difference was observed between the groups in terms of expression levels, except for GPR55 mRNA expression in the group of PND 7. Immunohistochemistry analysis showed that GPR55 is expressed in spermatids and spermatocytes in the mid-term tubules and spermatocytes in the late-stage tubules in groups of PND 56, 63, and 70. For GPR119, very intense positivity was observed only in spermatids in the mid-term (stage VII-VIII) tubules in the groups of PND 56, 63, and 70. No significant difference was observed in the number of GPR55 and GPR119 positive cells in testes from PND 56 through PND 70. Taken together, both GPR55 and GPR119 receptors are expressed throughout the rat testis development (PND 7 to 70). These results suggest that GPR55 and GPR119 are involved in the modulation of male reproductive function.
{"title":"EXPRESSION AND DISTRIBUTION OF GPR55 AND GPR119 DURING THE DEVELOPMENT OF RAT TESTIS","authors":"Hidayet TUTUN, Volkan İPEK, Musa TATAR, Ayşe KIZILYER, Muhammet Mükerrem KAYA","doi":"10.33988/auvfd.1121234","DOIUrl":"https://doi.org/10.33988/auvfd.1121234","url":null,"abstract":"G Protein-Coupled Receptors, GPR55 and GPR119 are widely distributed throughout the body and exert important biological functions. However, little is known about their roles in testis. This study aimed to examine the expression and distribution of GPR55 and GPR119 during the development of the rat testis. Sixty male Sprague Dawley rats (180–240 g) were divided into 10 groups as 7, 14, 21, 28, 35, 42, 49, 56, 63, and 70 postnatal days of age (PND) (six animals per group). The testicular expression of GPR55 and GPR119 has been investigated by immunohistochemistry, Western blot, and quantitative RT-PCR methods. We observed that GPR55 and GPR119 are expressed throughout the rat testis development from PND 7 to 70. However, no difference was observed between the groups in terms of expression levels, except for GPR55 mRNA expression in the group of PND 7. Immunohistochemistry analysis showed that GPR55 is expressed in spermatids and spermatocytes in the mid-term tubules and spermatocytes in the late-stage tubules in groups of PND 56, 63, and 70. For GPR119, very intense positivity was observed only in spermatids in the mid-term (stage VII-VIII) tubules in the groups of PND 56, 63, and 70. No significant difference was observed in the number of GPR55 and GPR119 positive cells in testes from PND 56 through PND 70. Taken together, both GPR55 and GPR119 receptors are expressed throughout the rat testis development (PND 7 to 70). These results suggest that GPR55 and GPR119 are involved in the modulation of male reproductive function.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135131567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Efe KURTDEDE, Mehmet Eray ALÇIĞIR, Ahmet Mahmut ALPEREN, Berk BARAN, Necat KUZU, Erman GÜLENDAĞ
Bu çalışmada, sıçanlarda 7,12-dimetilbenz[a]antrasen (DMBA) ile indüklenen bir meme tümörü modelinde vinkristin ve deli balın tek başına ve birlikte uygulanmasındaki baskılayıcı etkileri biyokimyasal, hematolojik ve histopatolojik olarak araştırıldı. 43-49 günlük toplam 72 rat 12 rattan oluşan 6 gruba ayrıldı.
Kontrol grubu (CG) sağlıklı sıçanlardan oluştu. Araç grubu (VG) sadece araç maddesi aldı ve kanser kontrol grubu (CCG) sadece DMBA aldı. Bal grubuna (HG) sadece Türk deli balı verildi. Vinkristin grubu (VinG) sadece vinkristin ve vinkristin-bal grubu (VHG) hem Türk deli balı hem de vinkristin aldı. 13 haftalık deneme süresinin son 4 haftasında Türk deli balı ve/veya vinkristin verildi.
Lökosit ve lenfosit sayıları CCG ve VG gruplarında anlamlı farklılık gösterdi. Alanin transaminaz ve total protein seviyeleri CCG ve VinG gruplarında daha yüksekti. Aspartat transaminaz CCG, HG ve VG gruplarında daha yüksekti. Kreatinin diğer tüm gruplarda HG grubundaki değere göre daha yüksekti. Kaspaz-3 ve Bax protein seviyeleri CG ve VG gruplarında daha yüksek, kaspaz-8 ve -9 ise daha düşüktü. Bax-xL, CCG grubunda daha fazla arttı. HG ve VinG gruplarında anaplazi azaldı, ancak apoptoz ve diğer hücresel hasarlar arttı.
Bu DMBA kaynaklı meme kanseri modelinde deli bal ve vincristine'in birlikte etkili terapötik ajanlar olarak kabul edilebileceği sonucuna varıldı.
{"title":"Evaluation of Tumor-Suppressive Properties and Apoptotic Functions of Mad Honey and Vincristine Applications in a Rat Model of Breast Cancer","authors":"Efe KURTDEDE, Mehmet Eray ALÇIĞIR, Ahmet Mahmut ALPEREN, Berk BARAN, Necat KUZU, Erman GÜLENDAĞ","doi":"10.33988/auvfd.1281608","DOIUrl":"https://doi.org/10.33988/auvfd.1281608","url":null,"abstract":"Bu çalışmada, sıçanlarda 7,12-dimetilbenz[a]antrasen (DMBA) ile indüklenen bir meme tümörü modelinde vinkristin ve deli balın tek başına ve birlikte uygulanmasındaki baskılayıcı etkileri biyokimyasal, hematolojik ve histopatolojik olarak araştırıldı. 43-49 günlük toplam 72 rat 12 rattan oluşan 6 gruba ayrıldı.
 Kontrol grubu (CG) sağlıklı sıçanlardan oluştu. Araç grubu (VG) sadece araç maddesi aldı ve kanser kontrol grubu (CCG) sadece DMBA aldı. Bal grubuna (HG) sadece Türk deli balı verildi. Vinkristin grubu (VinG) sadece vinkristin ve vinkristin-bal grubu (VHG) hem Türk deli balı hem de vinkristin aldı. 13 haftalık deneme süresinin son 4 haftasında Türk deli balı ve/veya vinkristin verildi.
 Lökosit ve lenfosit sayıları CCG ve VG gruplarında anlamlı farklılık gösterdi. Alanin transaminaz ve total protein seviyeleri CCG ve VinG gruplarında daha yüksekti. Aspartat transaminaz CCG, HG ve VG gruplarında daha yüksekti. Kreatinin diğer tüm gruplarda HG grubundaki değere göre daha yüksekti. Kaspaz-3 ve Bax protein seviyeleri CG ve VG gruplarında daha yüksek, kaspaz-8 ve -9 ise daha düşüktü. Bax-xL, CCG grubunda daha fazla arttı. HG ve VinG gruplarında anaplazi azaldı, ancak apoptoz ve diğer hücresel hasarlar arttı.
 Bu DMBA kaynaklı meme kanseri modelinde deli bal ve vincristine'in birlikte etkili terapötik ajanlar olarak kabul edilebileceği sonucuna varıldı.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136239376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim was to investigate the effects of common centaury (Centaurium erythraea) oil and laurel (Laurus nobilis) seed oil in a full-thickness excisional skin wound model in rats. In the study, 18 adult male Wistar rats were divided into three groups (n=6) as control (CO) group, common centaury oil (CCO) group, and laurel seed oil (LSO) group. Under general anesthesia, a full-thickness excisional wound (2.25 cm²) was created on the caudal of the interscapular region on the back of the rats. Treatments were applied topically once a day in all groups. Wound area measurements revealed that the use of CCO accelerated wound healing, while the use of LSO has disturbed. In the histopathological results, blood vessel formation, fiber synthesis, granulation and mononuclear cells in the wounds were higher in the CCO group than the other groups, and higher in the LSO group than the CO group. Biochemical results revealed differences between groups in TP, GLU and UREA values (p < 0.05). As a result of this study, it was determined that the topical use of common centaury oil accelerated wound healing, while laurel seed oil adversely affected wound healing in the experimental excisional full-thickness skin wound model in rats.
{"title":"Effects of common centaury (Centaurium erythraea) oil and laurel (Laurus nobilis) seed oil on full-thickness excisional skin wound healing in rats","authors":"Nazmiye SEMİZ, Mehmet Zeki Yılmaz DEVECİ","doi":"10.33988/auvfd.1085562","DOIUrl":"https://doi.org/10.33988/auvfd.1085562","url":null,"abstract":"The aim was to investigate the effects of common centaury (Centaurium erythraea) oil and laurel (Laurus nobilis) seed oil in a full-thickness excisional skin wound model in rats. In the study, 18 adult male Wistar rats were divided into three groups (n=6) as control (CO) group, common centaury oil (CCO) group, and laurel seed oil (LSO) group. Under general anesthesia, a full-thickness excisional wound (2.25 cm²) was created on the caudal of the interscapular region on the back of the rats. Treatments were applied topically once a day in all groups. Wound area measurements revealed that the use of CCO accelerated wound healing, while the use of LSO has disturbed. In the histopathological results, blood vessel formation, fiber synthesis, granulation and mononuclear cells in the wounds were higher in the CCO group than the other groups, and higher in the LSO group than the CO group. Biochemical results revealed differences between groups in TP, GLU and UREA values (p < 0.05). As a result of this study, it was determined that the topical use of common centaury oil accelerated wound healing, while laurel seed oil adversely affected wound healing in the experimental excisional full-thickness skin wound model in rats.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136240101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manuela AGOSTİNHO, Sheila RAHAL, Shayra BONATELLİ, Gustavo ROSA, Miriam TSUNEMİ, Vivian ZADRA, Maria Jaqueline MAMPRİM, Regina TAKAHİRA, Paulo SOUZA, Ivan SANTOS
This study aimed to evaluate the infrared thermography, arterial Doppler ultrasound, and Doppler echocardiography in healthy adult dogs exposed to a single Whole-body vibration (WBV) at different frequencies. Sixteen (16) healthy crossbreed dogs males of ages from 1 to 5 years, and weighing 16.3 to 24.5 kg were enrolled in the study. The dogs were exposed to a single WBV session at frequencies of 30 Hz (5 min), 40 Hz (5 min), and 50 Hz (5 min) with intervals between each frequency exposure of 10 min. The cutaneous temperature, arterial Doppler ultrasound, and Doppler echocardiography were evaluated 10 min before the WBV session, between each frequency, and 1 min after the last frequency. The cutaneous temperature of the regions of the superficial gluteal muscle and biceps femoris muscle of both hind limbs was obtained with an infrared camera. Resistive indexes of carotid and femoral arteries were determined using Doppler ultrasound, and Doppler echocardiography was used to assess the end-systolic volume and end-diastolic volume, heart rate, aortic blood flow velocity, and pulmonary artery flow velocity. Complete blood count and serum biochemical (alanine aminotransferase, creatinine, and creatine phosphokinase) were evaluated at 10 min before and 60 min after the end of the WBV session. Statistically significant differences were not found in any of the variables among the time points. In conclusion, the protocol of increasing vibration frequencies (30, 40, and 50 Hz) at short-period WBV can be considered appropriate since no change occurred in the parameters evaluated.
{"title":"Evaluation of infrared thermography, arterial Doppler ultrasound, and Doppler echocardiography in healthy adult dogs exposed to a single session of Whole-body vibration at different frequencies","authors":"Manuela AGOSTİNHO, Sheila RAHAL, Shayra BONATELLİ, Gustavo ROSA, Miriam TSUNEMİ, Vivian ZADRA, Maria Jaqueline MAMPRİM, Regina TAKAHİRA, Paulo SOUZA, Ivan SANTOS","doi":"10.33988/auvfd.1227213","DOIUrl":"https://doi.org/10.33988/auvfd.1227213","url":null,"abstract":"This study aimed to evaluate the infrared thermography, arterial Doppler ultrasound, and Doppler echocardiography in healthy adult dogs exposed to a single Whole-body vibration (WBV) at different frequencies. Sixteen (16) healthy crossbreed dogs males of ages from 1 to 5 years, and weighing 16.3 to 24.5 kg were enrolled in the study. The dogs were exposed to a single WBV session at frequencies of 30 Hz (5 min), 40 Hz (5 min), and 50 Hz (5 min) with intervals between each frequency exposure of 10 min. The cutaneous temperature, arterial Doppler ultrasound, and Doppler echocardiography were evaluated 10 min before the WBV session, between each frequency, and 1 min after the last frequency. The cutaneous temperature of the regions of the superficial gluteal muscle and biceps femoris muscle of both hind limbs was obtained with an infrared camera. Resistive indexes of carotid and femoral arteries were determined using Doppler ultrasound, and Doppler echocardiography was used to assess the end-systolic volume and end-diastolic volume, heart rate, aortic blood flow velocity, and pulmonary artery flow velocity. Complete blood count and serum biochemical (alanine aminotransferase, creatinine, and creatine phosphokinase) were evaluated at 10 min before and 60 min after the end of the WBV session. Statistically significant differences were not found in any of the variables among the time points. In conclusion, the protocol of increasing vibration frequencies (30, 40, and 50 Hz) at short-period WBV can be considered appropriate since no change occurred in the parameters evaluated.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135303834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Özge ARDIÇLI, Tuğçe SERİM KANAR, Serpil KAHYA DEMİRBİLEK, Ayşegül ÖZDEMİR, Serdar ERDOĞAN, Ahmet Gökhan COŞKUN, Tayfun CARLI
This study aimed to detect possible viral agents in backyard poultry where numerous commercial poultry companies are located. The backyard flock had non-vaccinated 30 meat-type chickens and four turkey pullets. Serum samples and tracheal swabs were taken from chickens and turkey pullets showing respiratory signs. Serum antibody levels were measured using commercial ELISA kits against Avian Influenza Virus (AIV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), Avian Metapneumovirus (AMPV), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), and Ornithobacterium rhinotracheale (ORT). In addition, tracheal swabs were tested for AIV serotypes H5, H7, and H9, NDV, IBV, AMPV, MG, MS, Pasteurella multocida, Avibacterium paragallinarum, and Bordetella avium by circular amplification technology (CAT). Anti-MS, -IBV, -MG, -NDV, -AMPV, and -ORT IgG antibodies were detected in some chicken sera, while anti-NDV, -MG, -MS, and -ORT IgG antibodies were detected in turkey sera. All avian tracheal swabs were positive for MG; however, IBV was only detected from chicken tracheal samples by CAT. The IBV strains were genotyped by sequencing a part of the S1 glycoprotein gene. Two isolates showed 99.35% and 98.69% nucleotide and 99.02% amino acid similarities with the 4/91 IBV strain. Two strains showed 99.35% nucleotide and 100% amino acid sequence identity to each other. Turkeys and chickens in the flock had MG and MG/IBV co-infections, respectively. Consequently, the presence of mutants of 4/91 IBV genotypes and MG found in backyard poultry could be a potential epidemiological source for commercial flocks in poultry integrations.
{"title":"Co-infection of S1 mutants of 4/91-like Infectious Bronchitis Virus with Mycoplasma gallisepticum in backyard poultry flock with a respiratory problem","authors":"Özge ARDIÇLI, Tuğçe SERİM KANAR, Serpil KAHYA DEMİRBİLEK, Ayşegül ÖZDEMİR, Serdar ERDOĞAN, Ahmet Gökhan COŞKUN, Tayfun CARLI","doi":"10.33988/auvfd.1252625","DOIUrl":"https://doi.org/10.33988/auvfd.1252625","url":null,"abstract":"This study aimed to detect possible viral agents in backyard poultry where numerous commercial poultry companies are located. The backyard flock had non-vaccinated 30 meat-type chickens and four turkey pullets. Serum samples and tracheal swabs were taken from chickens and turkey pullets showing respiratory signs. Serum antibody levels were measured using commercial ELISA kits against Avian Influenza Virus (AIV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), Avian Metapneumovirus (AMPV), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), and Ornithobacterium rhinotracheale (ORT). In addition, tracheal swabs were tested for AIV serotypes H5, H7, and H9, NDV, IBV, AMPV, MG, MS, Pasteurella multocida, Avibacterium paragallinarum, and Bordetella avium by circular amplification technology (CAT). Anti-MS, -IBV, -MG, -NDV, -AMPV, and -ORT IgG antibodies were detected in some chicken sera, while anti-NDV, -MG, -MS, and -ORT IgG antibodies were detected in turkey sera. All avian tracheal swabs were positive for MG; however, IBV was only detected from chicken tracheal samples by CAT. The IBV strains were genotyped by sequencing a part of the S1 glycoprotein gene. Two isolates showed 99.35% and 98.69% nucleotide and 99.02% amino acid similarities with the 4/91 IBV strain. Two strains showed 99.35% nucleotide and 100% amino acid sequence identity to each other. Turkeys and chickens in the flock had MG and MG/IBV co-infections, respectively. Consequently, the presence of mutants of 4/91 IBV genotypes and MG found in backyard poultry could be a potential epidemiological source for commercial flocks in poultry integrations.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136363003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Esra BOZKAYA, Mustafa TÜRK, Hüsamettin EKİCİ, Siyami KARAHAN
Cotinus coggyria is popularly known for its antiseptic, anti-inflammatory, antimicrobial, antihemorrhagic, and wound-healing properties. In this study, it was aimed to evaluate the phenolic contents, cytotoxicity/proliferation, hemolytic, antimicrobial, genotoxic, apoptotic, necrotic activities and in vivo wound healing effects of C.coggyria, a plant species known to have beneficial effects on wound healing. TOF-LC/MS analyzes revealed that the flavonoids and phenolic compounds in the methanol extract of C.coggyria leaves and stems were as follows: gallic acid, catechin, protocatechin acid, vanillic acid, ellagic acid, rosmarinic acid, quercetin, 4-hydroxybenzoic acid, chlorogenic acid, rutin and apigenin 7-glycoside. Water and methanol extracts from C.coggyria caused neither cytotoxicity nor genotoxicity in L929 fibroblasts with negligible apoptotic and necrotic effects. The water extract of C. coggyria had no hemolytic effect. The strongest antibacterial activity of methanol and water extracts was observed against E.coli with zone diameters of 9 mm and 8 mm, respectively. It was determined that the expression of GAG increased in the cells treated with C.coggyria stem extract compared to the control group. Collagen type I, type IV, fibronectin and elastin were detected in immunocytochemical staining. In the in vivo burn model, it was determined that the wounds treated with leaf and stem extracts healed faster than the control group. As a result, it has been seen that C.coggyria has antibacterial properties, especially against E.coli, without cytotoxic, genotoxic, hemolytic, apoptotic and necrotic activities, and C.coggyria is effective in wound healing by in vitro and in vivo studies
{"title":"Investigation of the biocompatibility and in vivo wound healing effect of Cotinus coggyria extracts","authors":"Esra BOZKAYA, Mustafa TÜRK, Hüsamettin EKİCİ, Siyami KARAHAN","doi":"10.33988/auvfd.1217177","DOIUrl":"https://doi.org/10.33988/auvfd.1217177","url":null,"abstract":"Cotinus coggyria is popularly known for its antiseptic, anti-inflammatory, antimicrobial, antihemorrhagic, and wound-healing properties. In this study, it was aimed to evaluate the phenolic contents, cytotoxicity/proliferation, hemolytic, antimicrobial, genotoxic, apoptotic, necrotic activities and in vivo wound healing effects of C.coggyria, a plant species known to have beneficial effects on wound healing. TOF-LC/MS analyzes revealed that the flavonoids and phenolic compounds in the methanol extract of C.coggyria leaves and stems were as follows: gallic acid, catechin, protocatechin acid, vanillic acid, ellagic acid, rosmarinic acid, quercetin, 4-hydroxybenzoic acid, chlorogenic acid, rutin and apigenin 7-glycoside. Water and methanol extracts from C.coggyria caused neither cytotoxicity nor genotoxicity in L929 fibroblasts with negligible apoptotic and necrotic effects. The water extract of C. coggyria had no hemolytic effect. The strongest antibacterial activity of methanol and water extracts was observed against E.coli with zone diameters of 9 mm and 8 mm, respectively. It was determined that the expression of GAG increased in the cells treated with C.coggyria stem extract compared to the control group. Collagen type I, type IV, fibronectin and elastin were detected in immunocytochemical staining. In the in vivo burn model, it was determined that the wounds treated with leaf and stem extracts healed faster than the control group. As a result, it has been seen that C.coggyria has antibacterial properties, especially against E.coli, without cytotoxic, genotoxic, hemolytic, apoptotic and necrotic activities, and C.coggyria is effective in wound healing by in vitro and in vivo studies","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135877378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An adult female hedgehog Erinaceus roumanicus, was found in a city traffic zone with serious injuries, most likely from a vehicle impact. Rapid diagnostics, including X-ray imaging, were performed at the veterinarian clinic to rescue the animal. Due to multiple traumatic injuries and poor prognosis, the animal was anesthetized for blood sampling and then euthanized. Polycythaemia, platelet aggregation, rare megakaryoblasts, neutrophilia, lymphocytosis, and a high red blood cell (RBC) and white blood cell (WBC) count were found in peripheral blood. Eosinophilia and the physiological phenomenon of emperipolesis were detected in the femur bone marrow, while the liver biopsy confirmed the extramedullary haematopoiesis (EMH). It has been noted that acute hypovolemic shock results in rapid changes in haematological and biochemical parameters. Given the increased vulnerability of small mammal fauna due to expanding urbanization, this finding may significantly improve care for their welfare and conservation.
{"title":"Polycythemia, emperipolesis and extramedullary haematopoiesis caused by acute shock: the first record in the Northern white-breasted hedgehog Erinaceus roumanicus Barrett-Hamilton, 1900","authors":"Damir SULJEVİC, Muhamed FOCAK, Lada LUKİC BİLELA","doi":"10.33988/auvfd.1242474","DOIUrl":"https://doi.org/10.33988/auvfd.1242474","url":null,"abstract":"An adult female hedgehog Erinaceus roumanicus, was found in a city traffic zone with serious injuries, most likely from a vehicle impact. Rapid diagnostics, including X-ray imaging, were performed at the veterinarian clinic to rescue the animal. Due to multiple traumatic injuries and poor prognosis, the animal was anesthetized for blood sampling and then euthanized. Polycythaemia, platelet aggregation, rare megakaryoblasts, neutrophilia, lymphocytosis, and a high red blood cell (RBC) and white blood cell (WBC) count were found in peripheral blood. Eosinophilia and the physiological phenomenon of emperipolesis were detected in the femur bone marrow, while the liver biopsy confirmed the extramedullary haematopoiesis (EMH). It has been noted that acute hypovolemic shock results in rapid changes in haematological and biochemical parameters. Given the increased vulnerability of small mammal fauna due to expanding urbanization, this finding may significantly improve care for their welfare and conservation.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135021646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Türkiye is exposed to international animal and semen movements. For this reason, Türkiye is very affected by diseases and hereditary disorders. State Farms is a state organization that raises and distributes breeding cattle. This study was aimed at investigating the presence and distribution of mutant alleles causing cholesterol deficiency (CD) disorder in Holstein cattle on state farms. For this purpose, blood and sperm samples were collected from 466 Holstein cattle. The real-time PCR method was used for genotyping. A total of seven cattle were found to be heterozygous. The frequency of the mutant allele was determined to be 0.75%. The kinship of four cattle carrying the mutant allele with Mauglin Storm, the bull in which this disease was first detected, was determined. The presence of cattle carrying the mutant allele in Türkiye is quite low compared to other countries. The Apolipoprotein-B (APOB) mutant allele was found at low frequencies and detected for the first time in Türkiye with this research. Therefore, it is necessary to develop control programs by screening other Holstein herds.
{"title":"Investigation of Hereditary Cholesterol Deficiency (CD) in Holstein Cattle at the State Farms in Türkiye","authors":"Ceyhan ÖZBEYAZ, Melike ÖZCAN","doi":"10.33988/auvfd.1295330","DOIUrl":"https://doi.org/10.33988/auvfd.1295330","url":null,"abstract":"Türkiye is exposed to international animal and semen movements. For this reason, Türkiye is very affected by diseases and hereditary disorders. State Farms is a state organization that raises and distributes breeding cattle. This study was aimed at investigating the presence and distribution of mutant alleles causing cholesterol deficiency (CD) disorder in Holstein cattle on state farms. For this purpose, blood and sperm samples were collected from 466 Holstein cattle. The real-time PCR method was used for genotyping. A total of seven cattle were found to be heterozygous. The frequency of the mutant allele was determined to be 0.75%. The kinship of four cattle carrying the mutant allele with Mauglin Storm, the bull in which this disease was first detected, was determined. The presence of cattle carrying the mutant allele in Türkiye is quite low compared to other countries. The Apolipoprotein-B (APOB) mutant allele was found at low frequencies and detected for the first time in Türkiye with this research. Therefore, it is necessary to develop control programs by screening other Holstein herds.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135163553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study is to determine the percentage of chicken meat in beef and chicken mixes, which is the most common type of beef adultery. In this context, both ground beef and beef sausages were prepared in mixtures containing chicken meat, increasing from 0.0% to 100.0% with 5.0% steps, and analyzed with a near infrared spectroscopy device. Optimum analysis conditions were determined as a result of the examination of a wide range of generated regression models. The success of the best regression model created for ground beef mixtures is as follows: RMSEC: 2.35, RMSEV: 3.36, R2C: 0.99, R2V: 0.98; and for the beef sausages is: RMSEC: 2.56, RMSEV: 3.66, R2C: 0.99, R2V: 0.98. As a result, chicken meat ratios in beef mixtures were detected with a margin of error of 2.05% and chicken meat ratios in beef sausages were detected with a margin of error of 2.12%.
{"title":"Rapid Determination of Chicken Meat Ratios in Beef Mixtures and Beef Sausages by Near Infrared Reflectance (NIR) Spectroscopy","authors":"Batuhan TARCAN, Özlem KÜPLÜLÜ","doi":"10.33988/auvfd.1200920","DOIUrl":"https://doi.org/10.33988/auvfd.1200920","url":null,"abstract":"The purpose of this study is to determine the percentage of chicken meat in beef and chicken mixes, which is the most common type of beef adultery. In this context, both ground beef and beef sausages were prepared in mixtures containing chicken meat, increasing from 0.0% to 100.0% with 5.0% steps, and analyzed with a near infrared spectroscopy device. Optimum analysis conditions were determined as a result of the examination of a wide range of generated regression models. The success of the best regression model created for ground beef mixtures is as follows: RMSEC: 2.35, RMSEV: 3.36, R2C: 0.99, R2V: 0.98; and for the beef sausages is: RMSEC: 2.56, RMSEV: 3.66, R2C: 0.99, R2V: 0.98. As a result, chicken meat ratios in beef mixtures were detected with a margin of error of 2.05% and chicken meat ratios in beef sausages were detected with a margin of error of 2.12%.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135597510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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