Effects of cryopreservation on enzyme activity of Exopalaemon carinicauda spermatozoa

Abstract

Exopalaemon carinicauda is one of the marine aquaculture species with high economic value in China.This paper studied the effects of cryopreservation(-196 ℃)on the enzyme activity(SDH,LDH,Na+K+-ATP,SOD,CAT,GR and Acrosin)in E.carinicauda spermatozoa,in order to provide a theoretical basis for cryopreservation effect on E.carinicauda spermatozoa,and lay a foundation for artificial propagation of E.carinicauda.We set up a control group(without cryoprotectant),and three experimental groups(DMSO(V/V)10%,12.5%,15%),detected the enzyme activities on day 0,1,3,5,7,15.The result showed that,after cryopreservation,in addition to GR,the other enzyme activities decreased significantly(P0.05),with the biggest drop in the control group.GR activity significantly increased in 7 days,and the control group was significantly higher than the experimental group(P0.05),declining again in 15 days.The group of 15% DMSO measured enzyme activities were higher than other groups,indicating that 15% DMSO had protective effect on enzyme of spermatozoa.15 days later,under the condition of 15% DMSO,the activities of SDH,LDH and Na+/K+-ATPase decreased from(28.500±1.453)U/mL,(1290.836±27.603)U/L and(2.605±0.232)μmol/(mg·h)to(15.300±0.950)U/mL,(363.713±13.943)U/L and(0.542±0.186)μmol/(mg·h)respectively;the activities of SOD and CAT decreased from(106.497±7.217)U/mL,(383.632±4.731)U/g to(17.036±0.321)U/mL and(166.940±1.910)U/g respectively;the activities of acrosin decreased from(3.521±0.010)μIU/106 to(1.212±0.043)μIU/106;but the activities of GR increased from(217.042±6.962)U/L to(302.787±24.558)U/L.From the decline range,cryopreservation had the biggest influence on SOD,followed by Na+/K+-ATPase.