Z. Cong, H. Jiang, G. Jin, T. Chen, W. Wang, Z. Tao, Nailin Yao, J. Xiong, F. Wu, Zhiwei Chen, Q. Wei
{"title":"Titration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques","authors":"Z. Cong, H. Jiang, G. Jin, T. Chen, W. Wang, Z. Tao, Nailin Yao, J. Xiong, F. Wu, Zhiwei Chen, Q. Wei","doi":"10.3969/J.ISSN.1671.7856.2011.04.003","DOIUrl":null,"url":null,"abstract":"Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously,and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation.Methods 10 Chinese-origin rhesus macaques were used in this study.Firstly,all the monkeys were screened by serological test to ensure that they were free of SIV,SRV,and STLV.Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately.Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR.Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i.v.successfully.Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously.This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.","PeriodicalId":10068,"journal":{"name":"中国比较医学杂志","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国比较医学杂志","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.3969/J.ISSN.1671.7856.2011.04.003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously,and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation.Methods 10 Chinese-origin rhesus macaques were used in this study.Firstly,all the monkeys were screened by serological test to ensure that they were free of SIV,SRV,and STLV.Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately.Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR.Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i.v.successfully.Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously.This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.
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