Pub Date : 2011-01-01DOI: 10.3969/J.ISSN.1671.7856.2011.04.004
Nailin Yao, W. Wang, Z. Cong, T. Chen, G. Jin, Z. Tao, Zhiwei Chen, Q. Wei
Objective To study the characteristics of RT-SHIV passaging in Chinese rhesus macaques and to establish a RT-SHIV /rhesus animal model providing an animal platform in evaluation of the effectiveness of HIV-1 drugs.Methods Four healthy rhesus macaques selected were negative to SIV,SRV and STLV-1.Two monkeys of them were infected with RT-SHIV intravenously.Blood samples were collected from the infected monkeys at the acute phase and CD8-PBMC were isolated,and then propagated RT-SHIV.The propagated RT-SHIV with PBMC were injected intravenously into the other two monkeys.Viral load in plasma,CD4 + /CD8 ratio and absolute number of CD4 + T lymphocytes and B lymphocytes were detected,and the RT gene variation was analyzed.Results Systemic infection was established in all of the four rhesus macaques,and the two passaged animals demonstrated more severe changes at the acute phase.There were three amino acid changed during infection and passages.Conclusion The results of our study provide basic information for the establishment of RT-SHIV /Chinese-origin rhesus monkeys models.
{"title":"RT-SHIV Infected and Passaged in Chinese-Origin Rhesus Macaque","authors":"Nailin Yao, W. Wang, Z. Cong, T. Chen, G. Jin, Z. Tao, Zhiwei Chen, Q. Wei","doi":"10.3969/J.ISSN.1671.7856.2011.04.004","DOIUrl":"https://doi.org/10.3969/J.ISSN.1671.7856.2011.04.004","url":null,"abstract":"Objective To study the characteristics of RT-SHIV passaging in Chinese rhesus macaques and to establish a RT-SHIV /rhesus animal model providing an animal platform in evaluation of the effectiveness of HIV-1 drugs.Methods Four healthy rhesus macaques selected were negative to SIV,SRV and STLV-1.Two monkeys of them were infected with RT-SHIV intravenously.Blood samples were collected from the infected monkeys at the acute phase and CD8-PBMC were isolated,and then propagated RT-SHIV.The propagated RT-SHIV with PBMC were injected intravenously into the other two monkeys.Viral load in plasma,CD4 + /CD8 ratio and absolute number of CD4 + T lymphocytes and B lymphocytes were detected,and the RT gene variation was analyzed.Results Systemic infection was established in all of the four rhesus macaques,and the two passaged animals demonstrated more severe changes at the acute phase.There were three amino acid changed during infection and passages.Conclusion The results of our study provide basic information for the establishment of RT-SHIV /Chinese-origin rhesus monkeys models.","PeriodicalId":10068,"journal":{"name":"中国比较医学杂志","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70180930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3969/J.ISSN.1671.7856.2011.04.001
W. Wang, Z. Cong, H. Liu, Z. Tao, Q. Liu, Q. Wei, Zhiwei Chen, C. Qin
Objective CD8+ T cells play an important role in some viral diseases,but the role of CD8+ T cells is not yet fully clear in the asymptomatic phase of HIV infection.The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion,and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups.Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0,3,and 7.Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry,the plasma viral load by real-time RT-PCR,and the cellular immunity by IFN-γ Elispot assay.Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion,but the reactions were not uniform.CD4 + T cells,the HIV-1 target cells,rebound and dropped slightly.The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection(plasma viral load and CD4 cells),which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection,but with some interindividual variation.
{"title":"Effect of CD8~+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection","authors":"W. Wang, Z. Cong, H. Liu, Z. Tao, Q. Liu, Q. Wei, Zhiwei Chen, C. Qin","doi":"10.3969/J.ISSN.1671.7856.2011.04.001","DOIUrl":"https://doi.org/10.3969/J.ISSN.1671.7856.2011.04.001","url":null,"abstract":"Objective CD8+ T cells play an important role in some viral diseases,but the role of CD8+ T cells is not yet fully clear in the asymptomatic phase of HIV infection.The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion,and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups.Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0,3,and 7.Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry,the plasma viral load by real-time RT-PCR,and the cellular immunity by IFN-γ Elispot assay.Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion,but the reactions were not uniform.CD4 + T cells,the HIV-1 target cells,rebound and dropped slightly.The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection(plasma viral load and CD4 cells),which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection,but with some interindividual variation.","PeriodicalId":10068,"journal":{"name":"中国比较医学杂志","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70181191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3969/J.ISSN.1671.7856.2011.04.003
Z. Cong, H. Jiang, G. Jin, T. Chen, W. Wang, Z. Tao, Nailin Yao, J. Xiong, F. Wu, Zhiwei Chen, Q. Wei
Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously,and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation.Methods 10 Chinese-origin rhesus macaques were used in this study.Firstly,all the monkeys were screened by serological test to ensure that they were free of SIV,SRV,and STLV.Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately.Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR.Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i.v.successfully.Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously.This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.
{"title":"Titration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques","authors":"Z. Cong, H. Jiang, G. Jin, T. Chen, W. Wang, Z. Tao, Nailin Yao, J. Xiong, F. Wu, Zhiwei Chen, Q. Wei","doi":"10.3969/J.ISSN.1671.7856.2011.04.003","DOIUrl":"https://doi.org/10.3969/J.ISSN.1671.7856.2011.04.003","url":null,"abstract":"Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously,and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation.Methods 10 Chinese-origin rhesus macaques were used in this study.Firstly,all the monkeys were screened by serological test to ensure that they were free of SIV,SRV,and STLV.Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately.Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR.Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i.v.successfully.Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously.This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.","PeriodicalId":10068,"journal":{"name":"中国比较医学杂志","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70181289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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