Fluoroquinolone resistant Salmonella enterica of poultry origin from south western states of Nigeria.

Abstract

The susceptibility of 41 Salmonella enterica isolates isolated from poultry disease outbreaks between April 2005 and August 2007 in Oyo and Ogun States Nigeria to ciprofloxacin, nalidixic acid, chloramphenicol,  streptomycin, kanamycin, ampicil in, neomycin, and tetracycline was determined. Using microtitre method for MIC determination, 22 resistance patterns were obtained. These include an octuple resistant pattern manifested by six isolates; three different septuple resistance patterns exhibited by four isolates, eight isolates manifested three dif erent sextuple resistance patterns, six isolates exhibited five different quintuple resistant patterns, three dif erent quadruple resistant patterns were presented by six isolates, five dif erent triple resistance patterns were shown by eight isolates, whereas two isolates showed double resistant pattern and a single resistance to ampicillin was seen in one isolate. A total of 30/41 (73.2%) were multi-drug resistant, showing resistance to four or eight antibiotics. R-type cip.amp.str.chl.kan.neo.tet.nal was the commonest seen in six isolates. Of al the isolates 26/ 41(63.4%) were ciprofloxacin resistant. This investigation reveals the widespread occurrence of multidrug resistant Salmonel a serovars from poultry disease outbreaks in Nigeria. Key words: Fluoroquinolone resistant Salmonel a enterica, Poultry, Multidrug-resistant. On a determine la susceptibilite de 41 isolats de Salmonella enteric isoles des maladies des epidemies de la volail e entre avril 2005 et aout 2007 dans les Etats d’Oyo et d’Ogun du Nigeria a la ciprofloxacine, a l’acide naledixique, au chloramphenicol, au streptomycine, au kanamycine, a l’ampicil ine, au neomycine et au tetracycline. En utilisant la methode de microtitration pour la determination de la CMI (concentration minimale inhibitrice), 22 profils de resistance ont ete obtenus. Il s’agit notamment du modele resistant octuple manifeste par six isolats; trois differents profils de resistance septuple exposes par quatre isolats, huit isolats qui manifestent trois differents profils de resistance aux sextuples, six isolats presentaient cinq differents modes de resistance quintuple, trois differents modes de quadruple resistants ont ete presentes par six isolats, cinq differents profils de resistance aux triples ont ete montrees par huit isolats alors que deux isolats ont montre un double modele resistant et une resistance a l’ampicil ine uniquement a ete observee dans un isolat. Un total de 30/41 (73,2%) resistait aux medicaments multiples, presentant une resistance a quatre ou huit antibiotiques. Le type R cip.amp.str.chl.kan.neo.tet.nal etait plus frequemment vu dans six isolats. De tous les isolats de 26/41 (63,4%) etaient resistant a la ciprofloxacine. Cette enquete revele la presence generalisee de la Salmonel a de type serologique multiresistant dans les epidemies de maladies de la volail e au Nigeria.