The function of the a-rich region of the alphasatellite associated with the cotton leaf curl disease in Pakistan

Q3 Agricultural and Biological Sciences Eurasian Journal of Biosciences Pub Date : 2009-07-01 DOI:10.5053/EJOBIOS.2009.3.0.19
M. Shahid, Liaqat Ali, S. Andleeb
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引用次数: 6

Abstract

A novel type of circular single stranded satellite-like DNA, known as alphasatellite (formerly known as DNA 1, was recently characterized and demonstrated to be associated with the monopartite Begomoviruses. Alphasatellite components are satellite like single stranded DNA (ssDNA) molecules associated with Begomoviruses (Geminiviridae) that require the betasatellite molecule to induce authentic disease symptoms in some hosts. Betasatellite is essential for induction of characteristic symptoms in plants. The function of alphasatellite in Begomovirus betasatellite infections remains unclear. It has been suggested that alphasatellite components may act to down regulate the virus infection by competing for cellular resources. Interestingly, they are closely related to the helper dependent Rep-encoding components of nanoviruses (a second family of single stranded, plant infecting DNA viruses), from which they are presumed to have been evolved. Alphasatellite molecules have two major sequence features. Firstly the component encodes a replication-associated protein (Rep), which is required to initiate the rolling circle replication. Consequently alphasatellite components are capable of self replication in host cells but require the helper Begomovirus to spread both within and between host plants. The second feature is a region of sequence rich in adenine (a-rich). To investigate the function of the a-rich sequence, this was deleted from the CLCuD alphasatellite by PCR mediated amplification. The a-rich deleted mutant of the alphasatellite remained capable of replication and systemic infection in plants, in the presence of a helper begomovirus. This indicates that the a-rich region is not required for replication or maintenance in plants.
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与巴基斯坦棉花卷叶病相关的α -卫星富a区功能
一种新型的环状单链卫星状DNA,称为alphasatellite(以前称为DNA 1),最近被鉴定并证明与单一性begomovirus有关。α卫星成分是与Begomoviruses (Geminiviridae)相关的卫星状单链DNA (ssDNA)分子,它需要β卫星分子在某些宿主中诱导真正的疾病症状。betasatellate对于诱导植物的特征症状是必不可少的。乙型肝炎病毒感染中乙型肝炎病毒的功能尚不清楚。有人认为,α卫星成分可能通过竞争细胞资源来下调病毒感染。有趣的是,它们与纳米病毒(单链植物感染DNA病毒的第二个家族)依赖于辅助性rep编码成分密切相关,据推测它们是从后者进化而来的。Alphasatellite分子有两个主要的序列特征。首先,该成分编码复制相关蛋白(Rep),这是启动滚动圈复制所必需的。因此,α卫星成分能够在宿主细胞中自我复制,但需要辅助begomavirus在宿主植物内部和宿主植物之间传播。第二个特征是序列区域富含腺嘌呤(a-rich)。为了研究富含a的序列的功能,通过PCR介导扩增将其从CLCuD α卫星中删除。在辅助begomavirus存在的情况下,富含a的α卫星缺失突变体仍然能够在植物中复制和全身性感染。这表明富a区不需要在植物中进行复制或维持。
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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