Isolation and characterization of salt-tolerant nitrogen-fixing microorganisms from food

Q3 Agricultural and Biological Sciences Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI:10.5053/EJOBIOS.2010.4.0.5
Girish Jadhav, D. Salunkhe, D. Nerkar, R. Bhadekar
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引用次数: 44

Abstract

Halophilic microorganisms are already in use for some biotechnological processes, such as commercial production of s-carotene, polymers (polyhydroxyalkanoates and polysaccharides), enzymes, compatible solutes etc. Considering their commercial importance, food samples (crude salt crystals and raw mango pickle) were used for isolation of halotolerant microorganisms. Two bacterial isolates obtained from food samples were examined for their ability to survive under stressed conditions and their growth response in increasing levels of NaCl (1 to 15% w/v), pH (5.0 to 10.0) and temperature (10 to 70°C). The isolates were rod shaped Gram-positive, salt-tolerant, non-halophilic, nitrogen-fixing strains. Different sugars such as glucose, fructose, maltose, sucrose, xylose and lactose were used to check for acid and gas production. The organisms were studied for their ability to hydrolyse substrates such as casein, starch, gelatin, etc. These organisms (i) grew well in SM basal salt medium and nitrogen-free semi-solid LGI medium (ii) tolerated 10-15% salt concentration (iii) produced acid from D-glucose, D-fructose and sucrose and (iv) utilized glycerol and citrate as carbon source, and v) survived acidic (pH 4-5) and alkaline (pH 9-10) conditions. The results suggested that there is potential to improve their performance as sources of industrially important enzymes. On the basis of morphological attributes and biochemical characteristics the isolates belonged to the genus Bacillus. The results of partial sequencing of 16S rRNA also revealed that the isolates 1 and 2 are closely related to Bacillus subtilis subsp. subtilis NCIB 3610T (97.9% pairwise similarity) and Bacillus sonorensis NRRL B-23154T (99.8% pair-wise similarity) respectively.
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食品中耐盐固氮微生物的分离与特性研究
嗜盐微生物已经用于一些生物技术过程,如s-胡萝卜素、聚合物(聚羟基烷酸酯和多糖)、酶、相容溶质等的商业生产。考虑到它们在商业上的重要性,使用食品样品(原盐晶体和生芒果泡菜)来分离耐盐微生物。研究了从食品样品中分离出的两种细菌在胁迫条件下的生存能力,以及它们在NaCl (1 ~ 15% w/v)、pH(5.0 ~ 10.0)和温度(10 ~ 70℃)升高条件下的生长反应。分离株为棒状革兰氏阳性、耐盐、非嗜盐、固氮菌株。不同的糖,如葡萄糖、果糖、麦芽糖、蔗糖、木糖和乳糖被用来检查酸和气体的产生。研究了这些生物水解酪蛋白、淀粉、明胶等底物的能力。这些生物(i)在SM基础盐培养基和无氮半固体LGI培养基中生长良好(ii)耐受10-15%的盐浓度(iii)由d -葡萄糖、d -果糖和蔗糖产生酸(iv)利用甘油和柠檬酸盐作为碳源,v)在酸性(pH 4-5)和碱性(pH 9-10)条件下存活。结果表明,作为工业上重要的酶的来源,它们的性能有可能得到改善。根据形态特征和生化特征,分离物属于芽孢杆菌属。16S rRNA部分测序结果也表明,分离物1和2与枯草芽孢杆菌亚种亲缘关系较近。枯草芽孢杆菌NCIB 3610T和索诺芽孢杆菌NRRL B-23154T的相似性分别为97.9%和99.8%。
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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