N. Fakharedine, Mouna Ouadghiri, M. Amar, P. Winterton, M. Hafidi, Y. Ouhdouch
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引用次数: 6
Abstract
Yeasts are widely used in various sectors of biomedical research, biotechnologies and environmental contexts. During the treatment of olive oil mill wastewater (OMW) by yeasts, extensive degradation of the polyphenols responsible for the highly toxic nature of this effluent occurs. In this study, one yeast isolate was selected for its ability to use the phenolic compounds in OMW as the sole carbon source for growth. The treatment of OMW using this isolate reduced the levels of polyphenols by about 50% and the fats by over 80%. Analysis of the polyphenol pool by HPLC revealed the presence of 6 compounds, most of which had disappeared after 5 and 25 days of treatment. Using API ID 32C, the taxonomic study of the selected isolate was found to be 99.9% identical to Candida valida. However, the molecular data using the Rep-PCR technique showed 83.6% similarity to Pichia membranifaciens and 80.6% to Pichia anomala.
酵母广泛应用于生物医学研究、生物技术和环境的各个领域。在酵母处理橄榄油厂废水(OMW)的过程中,造成这种废水剧毒性质的多酚会发生广泛降解。在本研究中,选择了一种酵母分离物,因为它能够利用OMW中的酚类化合物作为生长的唯一碳源。使用该分离物处理的OMW使多酚含量降低了约50%,脂肪含量降低了80%以上。高效液相色谱法分析多酚池中存在6种化合物,其中大部分在处理5天和25天后消失。使用API ID 32C进行分类研究,发现所选分离物与假丝酵母(Candida valida)的同源性为99.9%。然而,利用Rep-PCR技术的分子数据显示,与膜性毕赤酵母的相似性为83.6%,与异常毕赤酵母的相似性为80.6%。
期刊介绍:
EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology