Characterization of cellulase enzyme produced by Chaetomium sp. isolated from books and archives

Q3 Agricultural and Biological Sciences Eurasian Journal of Biosciences Pub Date : 2015-07-01 DOI:10.5053/EJOBIOS.2015.9.0.7
Moza AL-KHAROUSI, N. Sivakumar, A. Elshafie
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引用次数: 13

Abstract

Background: Cellulase is an important industrial enzyme used to degrade cellulosic biomass. The demand for cellulase enzyme is continuously increasing because of its applications in various industries. Hence, screening of cellulase producing microorganisms from different sources has gained significant importance. Material and Methods: In this study, fungi isolated from books and archives were screened for their cellulase producing abilities. Four different fungi were isolated from books and archives using potato dextrose agar. Screening of these isolates for cellulase production was carried out using carboxymethyl cellulose broth. The most efficient fungus was subjected to cellulase fermentation and enzymes produced were purified and partially characterized. Results: Four different fungi, Chaetomium sp., Aspergillus niger, Aspergillus nidulans and Penicillium sp., were isolated from books and archives. All the isolates were tested for their ability to produce cellulase enzyme. During the primary screening Chaetomium sp. showed good growth and higher cellulase activity (155.3±25.6 U/mL) in carboxymethyl cellulose medium than the other fungi. The cellulase fermentation study was conducted with Chaetomium sp. using carboxymethyl cellulose as a substrate. During the stationary phase (144 h) of the growth, the cellulase activity of Chaetomium sp. was significantly high. The maximum mycelial weight of this fungi was obtained at 168 h. Viscosity of the Chaetomium sp. inoculated fermentation medium continuously decreased until 144 h because of the degradation of carboxymethyl cellulose. During cellulase fermentation, pH increased from the initial neutral pH to 8.5. Purified cellulase showed a specific activity of 7.3 U/mg. It exhibited maximum activity at 20°C and was stable between pH 5 and 9. Conclusions: Books and archives could be a good source for the isolation of cellulase producing fungi.
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从文献和文献中分离的毛藻生产纤维素酶的特性
背景:纤维素酶是一种重要的用于降解纤维素生物质的工业酶。由于纤维素酶在各行各业的应用,对其需求量不断增加。因此,从不同来源筛选产生纤维素酶的微生物具有重要意义。材料和方法:在本研究中,对从书籍和档案中分离的真菌进行了纤维素酶生产能力的筛选。利用马铃薯葡萄糖琼脂从书籍和档案中分离出四种不同的真菌。利用羧甲基纤维素肉汤对这些分离菌株进行了纤维素酶生产筛选。对效率最高的真菌进行纤维素酶发酵,并对所产生的酶进行纯化和部分表征。结果:从文献资料中分离到毛菌、黑曲霉、灰曲霉和青霉4种不同的真菌。对所有菌株进行了产纤维素酶能力的测试。初步筛选毛毛菌在羧甲基纤维素培养基中生长良好,纤维素酶活性(155.3±25.6 U/mL)高于其他真菌。以毛藻为原料,以羧甲基纤维素为底物进行纤维素酶发酵研究。在生长的固定阶段(144 h),毛藻的纤维素酶活性显著提高。该菌菌丝体质量在168 h时达到最大值。接种毛毛菌发酵培养基的粘度持续下降,直至144 h,因为羧甲基纤维素的降解。在纤维素酶发酵过程中,pH由初始的中性pH增加到8.5。纯化后的纤维素酶比活性为7.3 U/mg。在20℃时活性最高,pH值在5 ~ 9之间稳定。结论:文献资料是分离产纤维素酶真菌的良好资料来源。
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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