Source identification and entry pathways of banned antibiotics nitrofuran and chloramphenicol in shrimp value chain of Bangladesh

Q3 Agricultural and Biological Sciences Eurasian Journal of Biosciences Pub Date : 2014-07-01 DOI:10.5053/EJOBIOS.2014.8.0.7
M. J. Islam, A. Liza, A. Reza, M. Reza, M. N. A. Khan, M. Kamal
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引用次数: 30

Abstract

Background: Contamination with residues of banned carcinogenic antibiotic drugs like nitrofuran metabolites and chloramphenicol (CAP) in frozen shrimp products has become a major concern of food safety for exporting countries. In the present study an approach was taken to identify the sources of such harmful antibiotics in the shrimp value chain of Bangladesh, one of the major shrimp countries. Material and Methods: Inputs of farms and hatchery systems including feed, feed additives, feed ingredients and therapeutic agents were thought to be the sources of contagion. Fish and shrimp feed, feed ingredients, sediment and water samples of shrimp hatcheries and farms were, therefore, analyzed for 3-Amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 3-Amino-2-oxazolidinone (AOZ), 1-Amino-hydantoin (AHD), Semicarbazide (SEM) and chloramphenicol (CAP) to identify their source and entry pathways. About 500 g of each 160 feed and feed ingredients were collected in pyrogens free polyethylene sealed bag and transported to Fish Inspection and Quality Control (FIQC) laboratory, Dhaka, Bangladesh. Whereas 500 mL of each 250 soils and water sample were collected from hatcheries. Sample preparation and residual metabolites analysis were conducted using validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical assays on an Waters Alliance 2695 series HPLC and Quattro Micro, API mass spectrometer instrumentation (Waters Corporation, USA). Results: Among the analyzed 160 feed samples, 38 were found contaminated with CAP and/or nitrofuran metabolites (AMOZ, AOZ, A H D and SEM), where 11,10, 8, and 9 samples were for shrimp feed, fish feed, poultry feed and feed ingredients. Imported feed ingredients contained with protein concentrates of improper quality were found contaminate with higher level of SEM. Although hatcheries were found free from contamination, whereas sediment and water samples of many shrimp farms were found contaminated with high levels of SEM and CAP. Conclusions: It could be narrated that antibiotic contamination of shrimp products were the use of antibiotic contaminated feed and feed ingredients in the farms; use of poultry litter to fertilize ponds during mixed culture, because poultry were fed with antibiotic medicated feed from zero day of feeding and indiscriminate use of insecticides and pesticides at nearby agricultural farms.
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孟加拉国虾类价值链中被禁抗生素硝基呋喃和氯霉素的来源识别和进入途径
背景:冷冻虾产品中硝基呋喃代谢物和氯霉素(CAP)等禁用致癌性抗生素残留污染已成为出口国关注的主要食品安全问题。在本研究中,采取了一种方法来确定孟加拉国虾类价值链中这种有害抗生素的来源,孟加拉国是主要的虾类生产国之一。材料和方法:农场和孵化场系统的投入,包括饲料、饲料添加剂、饲料成分和治疗剂,被认为是传染源。为此,对鱼虾饲料、饲料原料、对虾养殖场沉积物和水样进行了3-氨基-5- morpholinomemethyl -2-oxazolidinone (AMOZ)、3-氨基-2- oxazolidone (AOZ)、1-氨基-hydantoin (AHD)、氨基脲(SEM)和氯霉素(CAP)的来源和进入途径分析。每160种饲料和饲料原料中约500克装在无热原的聚乙烯密封袋中,运往孟加拉国达卡的鱼类检验和质量控制(FIQC)实验室。而每250个土壤和水样中有500毫升是从孵化场采集的。样品制备和残留代谢物分析采用经验证的液相色谱-串联质谱(LC-MS/MS)分析方法,采用Waters Alliance 2695系列高效液相色谱和Quattro Micro, API质谱仪(Waters Corporation, USA)。结果:在160份饲料样品中,发现CAP和(或)硝基呋喃代谢物(AMOZ、AOZ、A - H - D和SEM)污染38份,其中对虾饲料、鱼饲料、家禽饲料和饲料配料分别有11份、10份、8份和9份。进口饲料原料中含有质量不合格的蛋白质浓缩物,扫描电镜检测结果较高。虽然孵化场未发现污染,但许多养殖场的底泥和水样中发现了高水平的SEM和CAP污染。结论:虾类产品的抗生素污染可能是由于养殖场使用了抗生素污染的饲料和饲料原料;在混合养殖期间使用家禽粪便给池塘施肥,因为家禽从饲养的第一天起就被喂食抗生素药物饲料,而且附近的农场不加选择地使用杀虫剂和杀虫剂。
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
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期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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