The antiproliferative and cytotoxic effects of curcumin on human cervical cancer Hep2C cell line

Abstract

Objective: In this study, antiproliferative and cytotoxic effects of different concentrations of curcumin on cervical cancer Hep2C cells were investigated with microscopic methods and MTT assay. Methods: Hep2C (human carcinoma cancer cell line, ATCC:CCL-23) cells were cultured. For cytotoxicity evaluation Hep2C cells exposed to curcumin at different concentrations of 30 µg/ml, 15 µg/ml, 7.5 µg/ml, 3.7 µg/ml, 1.9 µg/ml, 0.9 µg/ml, 0.45 µg/ml for 24 hours These Hep2C cells are evaluated with MTT assay. The IC 50 value of the agent for 24 h of exposure was detected. The graph of the absorbance data obtained by the Spectramax I3 device. Viability values of Hep2C cells calculated from the absorbances obtained from MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay are gained. The preparations were observed based on changes in nuclei and structures using an inverted microscope (Leica Microsystems). Nontreated cells were used as negative control and for positive control Hep2C cells were exposed to ammonium molibdate (1mg/ml) for the above given incubation period. hücre kültürü Results: High doses of curcumin µg/ml, 15 µg/ml, 7.5 µg/ml) showed high antiproliferative and cytotoxic effects on Hep2C cells. The antiproliferative and cytotoxic effects were not observed on cervical cancer Hep2C cells treated with lower concentrations of curcumin. Conclusion: Curcumin has been shown that it is non-toxic, can be used as a highly antioxidant and anti-inflammatory agent and has multifaced therapeutic-pharmacological effects. However, researches on the antiproliferative, anti-cancer effects of curcumin in cervical cancer cells is not sufficient. The present study evaluates the antiproliferative and cytotoxic effects of curcumin on human cervical cancer Hep2C cells as the first time. The results of our study support these effects of curcumin on Hep2C cells in a concentration-dependent