Ficus carica extract causes cell cycle arrest and induces apoptosis in MG-63 and HT-29 cancer cell lines

Q4 Medicine Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2023-01-01 DOI:10.5505/turkhijyen.2023.93357

Tuğba Yalçınkaya, L. Kozacı, Ahmet Çarhan

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Abstract

Objective: Ficus carica (Fig) is a leafy tree of Moraceae, which is used in local traditional medicine to treat various diseases. Recent studies revealed an impressive anti-cancer efficiency of F. carica extracts in different types of cancer cells. This study aimed to investigate the anti-cancer effects of F. carica extract on colon cancer cell line HT-29 and bone cancer cell line MG-63. Methods: The human colon cancer cell line HT-29 and bone cancer cell line MG-63 were used to investigate the effects of F. carica extract. The effects of F. carica on cell viability were evaluated using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide] assay. Complementary analyses for evaluating membrane integrity and toxicity were performed by estimating LDH (lactate dehydrogenase) assay. Mechanisms of cell death were analyzed using Muse TM Annexin-V and TUNEL assays. Cell-cycle distribution was examined using flow cytometri analysis. Results: The results demonstrated that F. carica extract caused a significant decrease in cell viability in cancer cells in a dose-and time-dependent fashion, but

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无花果提取物对MG-63和HT-29细胞株的细胞周期阻滞和细胞凋亡具有诱导作用

目的:无花果(Ficus carica, Fig)是桑科的一种阔叶乔木，在当地传统医学中用于治疗多种疾病。最近的研究表明，乳香提取物对不同类型的癌细胞具有令人印象深刻的抗癌效果。本研究旨在探讨鸢尾提取物对结肠癌细胞株HT-29和骨癌细胞株MG-63的抗癌作用。方法:以人结肠癌细胞株HT-29和骨癌细胞株MG-63为实验对象，研究鸢尾提取物的抗肿瘤作用。采用MTT[3-(4,5-二甲基噻唑-2-基)-2,5二苯基溴化四氮唑]测定法评价了carica对细胞活力的影响。通过乳酸脱氢酶(LDH)测定来评估膜的完整性和毒性。采用Muse TM Annexin-V和TUNEL分析细胞死亡机制。流式细胞仪检测细胞周期分布。结果:乳香提取物显著降低肿瘤细胞活力，且呈剂量和时间依赖性

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Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Medicine-Infectious Diseases

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