Dominance of Pro-Inflammatory Cytokines Over Anti-Inflammatory Ones in Placental Bed of Creta Cases.

Q3 Medicine Journal of Microscopy and Ultrastructure Pub Date : 2022-11-14 eCollection Date: 2024-01-01 DOI:10.4103/jmau.jmau_76_21
Ahmed A M Abdel-Hamid, Yaser Mesbah, Mona F M Soliman, Alaa El-Din L Firgany
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Abstract

Objective: The objective of the study is to investigate changes occurring in key inflammatory cytokines at molecular level (including genetic and protein) in placental bed of placenta creta compared to that of normal placenta and their correlation to interstitial extravillous trophoblasts (EVT) number.

Subjects and methods: Case-control study including placentas of patients with invasive placentation (creta placentas, n = 19) compared with those of normal placentation (n = 19). Besides routine histology and immunocytochemistry detection (cytokeratin-7 [CK-7]), addition to biochemical evaluation of expression of various cytokines including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), IL6, IL-1RA, IL-8, IL-10, and IL-13 was carried out.

Results: Routine histological examination of placentas of creta cases revealed CK-7+ extravillous trophoblasts (EVT) penetrating deeply the myometrium with various histopathological arrangements and trophoblastic vascular invasion of the deep myometrial blood vessels. A significant increase (P < 0.05) in the mRNA expression of TNF-α, IL-1 β, and IL6 with an insignificant decrease in placental bed IL-1RA, IL-8, IL-10, and IL-13 was observed in creta cases compared to the control ones. A corresponding significant increase was detected in the protein levels of TNF-α, IL-1 β, and IL-6 as well as an insignificant decrease in placental bed IL-1RA, IL-8, IL-10, and IL-13 in creta cases compared to the normal ones. Moreover, we displayed a significant positive correlation (P < 0.05) between interstitial EVT number and mRNA expression of almost all pro-inflammatory cytokines with negative but insignificant correlation with anti-inflammatory cytokines in creta cases.

Conclusion: The upregulated pro-inflammatory cytokines and the correlation of their expression with the increased interstitial EVT provide a supporting evidence of their potentially more relevant role in the development of placenta creta than the anti-inflammatory ones.

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克里塔病例胎盘床中促炎细胞因子的优势大于抗炎细胞因子。
研究目的本研究的目的是探讨与正常胎盘相比,浸润性胎盘胎盘床中主要炎症细胞因子在分子水平(包括基因和蛋白质)上发生的变化及其与间质外滋养细胞(EVT)数量的相关性:病例对照研究:将侵袭性胎盘患者的胎盘(克雷塔胎盘,n = 19)与正常胎盘患者的胎盘(n = 19)进行比较。除了常规组织学和免疫细胞化学检测(细胞角蛋白-7 [CK-7])外,还对各种细胞因子的表达进行了生化评估,包括肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL6、IL-1RA、IL-8、IL-10和IL-13:对Creta病例胎盘的常规组织学检查显示,CK-7+绒毛膜外滋养细胞(EVT)深入子宫肌层,具有不同的组织病理学排列,滋养细胞血管侵入子宫肌层深部血管。与对照组相比,观察到Creta病例中TNF-α、IL-1 β和IL6的mRNA表达明显增加(P<0.05),而胎盘床IL-1RA、IL-8、IL-10和IL-13的表达减少不明显。与正常病例相比,TNF-α、IL-1 β和IL-6的蛋白水平在creta病例中也有相应的显著升高,而胎盘床IL-1RA、IL-8、IL-10和IL-13的下降不明显。此外,我们还发现,在creta病例中,间质EVT数量与几乎所有促炎细胞因子的mRNA表达呈显著正相关(P<0.05),而与抗炎细胞因子呈负相关,但不显著:结论:促炎细胞因子的上调及其表达与间质EVT增加的相关性提供了支持性证据,证明在胎盘早剥的发生过程中,促炎细胞因子可能比抗炎细胞因子发挥更重要的作用。
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