Montanide TM Gel01 ST Adjuvant Enhances PRRS Modified Live Vaccine Efficacy by Regulating Porcine Humoral and Cellular Immune Responses

Xiangdong Li, A. Galliher-Beckley, J. Nietfeld, K. Faaberg, Jishu Shi
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引用次数: 11

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a devastating disease caused by the PRRS virus. The MontanideTM class of flexible polymeric adjuvants has recently been shown to enhance protective immunity against PRRSV infection in piglets when used in combination with PRRS modified live vaccines (MLV). In this study, we explored the efficacy and immunological mechanisms of protection of MontanideTM Gel01 ST (Gel01) adjuvanted modified live PRRSV vaccine in pigs challenged with two genetically distinct strains of PRRSV. Gel01-MLV reduced lymph node pathology scores in pigs challenged with VR-2332 (parental strain of MLV vaccine) but not that in pigs challenged with MN184A (heterologous strain), when compared to that in pigs vaccinated with un-adjuvanted MLV. Pigs vaccinated with Gel01-MLV had higher levels of PRRS-specific antibodies, as measured by IDEXX ELISA and virus neutralizing antibodies, after vaccination and VR-2332 challenge. In addition, pigs vaccinated with Gel01-MLV had decreased levels of IFN-γ, IL-10, and T-regulatory lymphocytes in the blood as compared to that in pigs vaccinated with MLV alone. Interestingly, we found that addition of Gel01 did not change the profile of other T lymphocyte populations after PRRSV challenge. These results demonstrate that the MLV adjuvanted with Gel01 provides enhanced protection against homologous PRRSV infection, possibly by regulating the production of PRRSV-specific antibodies and cytokines involved in the development of T-regulatory cells. Thus, Gel01 ST is a promising adjuvant that can be formulated with PRRSV MLV vaccines to reduce disease severity and tissue damage caused by PRRSV infection in pigs.
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Montanide TM Gel01 ST佐剂通过调节猪体液和细胞免疫反应增强PRRS修饰活疫苗的效力
猪繁殖与呼吸综合征(PRRS)是由PRRS病毒引起的一种毁灭性疾病。MontanideTM类柔性聚合佐剂最近被证明与PRRS修饰活疫苗(MLV)联合使用可增强仔猪对PRRSV感染的保护性免疫。在这项研究中,我们探讨了蒙塔尼特™Gel01 ST (Gel01)佐剂修饰PRRSV活疫苗对两种基因不同的PRRSV毒株攻毒的猪的保护效果和免疫学机制。Gel01-MLV降低了接种VR-2332 (MLV疫苗亲本株)的猪的淋巴结病理评分,但与接种未佐剂MLV的猪相比,接种MN184A(异源株)的猪的淋巴结病理评分没有降低。通过IDEXX ELISA和病毒中和抗体检测,接种Gel01-MLV的猪在接种疫苗和VR-2332攻毒后具有更高水平的prrs特异性抗体。此外,与单独接种MLV的猪相比,接种Gel01-MLV的猪血液中IFN-γ、IL-10和t调节淋巴细胞的水平降低。有趣的是,我们发现添加Gel01并没有改变PRRSV攻击后其他T淋巴细胞群的特征。这些结果表明,Gel01佐剂的MLV对同源PRRSV感染具有增强的保护作用,可能是通过调节PRRSV特异性抗体的产生和参与t调节细胞发育的细胞因子来实现的。因此,Gel01 ST是一种很有前景的佐剂,可以与PRRSV MLV疫苗配制,以降低猪PRRSV感染引起的疾病严重程度和组织损伤。
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