A New Immunosuppressive Protein in Pig Spleen

H. Fu, Juan Feng, S. Fan
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Abstract

Chronic stress has been the popular phenomenon in the modern society. But we have not yet resolved the problem effectively. A novel protein in pig spleen, which possesses immunosuppressive activity, has been purified in this study. Its molecular weight about 200 kDa and three subunits composition were fractionated by SDS-PAGE. The protein inhibited mouse T-lymphocyte proliferation by the induced concanavalin A (ConA) and regulatory volume decrease (RVD), which may be due to the decreased Kv1.3 expression and activity. In addition, we have raised successfully its polyclonal antibody, which could reverse the inhibited lymphocyte proliferation by the immuno-suppressive protein. And the antibody could be used to coat plate for ELISA assay. Therefore, the antibody will have the potential to provide a basis for applying to clinical stress diseases in the future.
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一种新的猪脾脏免疫抑制蛋白
慢性压力已经成为现代社会的普遍现象。但是我们还没有有效地解决这个问题。本研究纯化了猪脾脏中一种具有免疫抑制活性的新蛋白。SDS-PAGE对其分子量约200 kDa和3个亚基组成进行了分离。该蛋白通过诱导豆蛋白A (ConA)和调节体积减少(RVD)抑制小鼠t淋巴细胞增殖,这可能是由于Kv1.3的表达和活性降低所致。此外,我们成功地制备了其多克隆抗体,该抗体可以逆转免疫抑制蛋白对淋巴细胞增殖的抑制。该抗体可用包被板进行酶联免疫吸附试验。因此,该抗体将来有可能为临床应激性疾病的应用提供依据。
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